Product nameAnti-WSTF antibody
See all WSTF primary antibodies
DescriptionRabbit polyclonal to WSTF
Tested applicationsSuitable for: WB, IP, IHC-P, ICCmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Opossum, Orangutan
A region between residues 1 and 50 of Human WSTF (SwissProt entry Q9UIG0)
- Whole cell lysate from HeLa cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70263 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 171 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|ICC||1/100 - 1/500.
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE cells. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells in cytospin preparations.
FunctionAtypical tyrosine-protein kinase that plays a central role in chromatin remodeling and acts as a transcription regulator. Involved in DNA damage response by phosphorylating 'Tyr-142' of histone H2AX (H2AXY142ph). H2AXY142ph plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. In the complex, it mediates the recruitment of the WICH complex to replication foci during DNA replication. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. In the WINAC complex, plays an essential role by targeting the complex to acetylated histones, an essential step for VDR-promoter association.
Tissue specificityUbiquitously expressed with high levels of expression in heart, brain, placenta, skeletal muscle and ovary.
Involvement in diseaseNote=BAZ1B is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of BAZ1B may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
Sequence similaritiesBelongs to the WAL family. BAZ1B subfamily.
Contains 1 bromo domain.
Contains 1 DDT domain.
Contains 1 PHD-type zinc finger.
Contains 1 WAC domain.
Developmental stageExpressed at equal levels in 19-23 weeks old fetal tissues.
DomainThe N-terminal part (1-345), including the WAC domain and the C motif, mediates the tyrosine-protein kinase activity.
The bromo domain mediates the specific interaction with acetylated histones.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Accumulates in pericentromeric heterochromatin during replication. Targeted to replication foci throughout S phase via its association with PCNA.
- Information by UniProt
- baz1b antibody
- BAZ1B_HUMAN antibody
- Bromodomain adjacent to zinc finger domain protein 1B antibody
All lanes : Anti-WSTF antibody (ab70263) at 0.05 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 5 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 50 µg
Predicted band size: 171 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Additional bands at: 140 kDa, 190 kDa, 300 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling WSTF with ab70263 at 1/200 (1µg/ml). Detection: DAB.
1 mg of whole cell lysate from HeLa cells was immunoprecipitated using ab70263 at 3ug/mg lysate (lane 1) or a control rabbit Ig (lane 2). The subsequent blot was carried with ab70263 at 0.05ug/ml.
ab70263 has not yet been referenced specifically in any publications.