Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-WSTF antibody [EP1704Y] - BSA and Azide free (ab235388)

Overview

  • Product name

    Anti-WSTF antibody [EP1704Y] - BSA and Azide free
    See all WSTF primary antibodies
  • Description

    Rabbit monoclonal [EP1704Y] to WSTF - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human WSTF (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Wild-type HAP1, HeLa (ab150035), and PC12 cell lysates. ICC/IF: HeLa cells. IHC-P: Human breast cancer, mouse cardiac muscle and rat cardiac muscle tissues.
  • General notes

    Ab235388 is the carrier-free version of ab51256. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab235388 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab235388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 185 kDa (predicted molecular weight: 171 kDa).
IHC-P Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Atypical tyrosine-protein kinase that plays a central role in chromatin remodeling and acts as a transcription regulator. Involved in DNA damage response by phosphorylating 'Tyr-142' of histone H2AX (H2AXY142ph). H2AXY142ph plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. In the complex, it mediates the recruitment of the WICH complex to replication foci during DNA replication. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. In the WINAC complex, plays an essential role by targeting the complex to acetylated histones, an essential step for VDR-promoter association.
    • Tissue specificity

      Ubiquitously expressed with high levels of expression in heart, brain, placenta, skeletal muscle and ovary.
    • Involvement in disease

      Note=BAZ1B is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of BAZ1B may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
    • Sequence similarities

      Belongs to the WAL family. BAZ1B subfamily.
      Contains 1 bromo domain.
      Contains 1 DDT domain.
      Contains 1 PHD-type zinc finger.
      Contains 1 WAC domain.
    • Developmental stage

      Expressed at equal levels in 19-23 weeks old fetal tissues.
    • Domain

      The N-terminal part (1-345), including the WAC domain and the C motif, mediates the tyrosine-protein kinase activity.
      The bromo domain mediates the specific interaction with acetylated histones.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus. Accumulates in pericentromeric heterochromatin during replication. Targeted to replication foci throughout S phase via its association with PCNA.
    • Information by UniProt
    • Database links

    • Alternative names

      • baz1b antibody
      • BAZ1B_HUMAN antibody
      • Bromodomain adjacent to zinc finger domain protein 1B antibody
      • hWALP 2 antibody
      • hWALP-2 antibody
      • hWALP2 antibody
      • transcription factor WSTF antibody
      • Tyrosine-protein kinase BAZ1B antibody
      • WALP-2 antibody
      • WALP2 antibody
      • WBRS 9 antibody
      • WBRS-9 antibody
      • WBRS9 antibody
      • WBSC 10 antibody
      • WBSC-10 antibody
      • WBSC10 antibody
      • WBSCR10 antibody
      • WBSCR9 antibody
      • Williams Beuren syndrome chromosome region 9 protein antibody
      • Williams syndrome transcription factor antibody
      • Williams-Beuren syndrome chromosomal region 10 protein antibody
      • Williams-Beuren syndrome chromosomal region 9 protein antibody
      • WSTF antibody
      see all

    Images

    • This WB data was generated using the same anti-WSTF antibody clone, EP1704Y, in a different buffer formulation (cat# ab51256).

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: WSTF knockout HAP1 cell lysate (20 µg) 
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: PC12 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab51256 observed at 175 kDa. Red - loading control, ab18058, observed at 124 kDa.

      ab51265 was shown to recognize WSTF in wilt-type cells along with additional cross-reactive bands as signal was lost in WSTF knockout samples. Wild-type and WSTF knockout samples were subjected to SDS-PAGE. ab51256 and ab18058 (loading control to vinculin) were diluted 1/15 000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling WSTF with ab51256 at a dilution of 1/1000. Cells were fized with 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody at a dilution of 1/1000.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51256).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue labelling WSTF with ab51256 at a dilution of 1/500. Heat mediated antigen retrieval was performed using citrate buffer, pH 6.0. A ready to use HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody. Counter stained with hematoxylin.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51256).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac musle tissue labelling WSTF with ab51256 at a dilution of 1/500. Heat mediated antigen retrieval was performed using citrate buffer, pH 6.0. A ready to use HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody. Counter stained with hematoxylin.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51256).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cardiac muscle tissue labelling WSTF with ab51256 at a dilution of 1/500. Heat mediated antigen retrieval was performed using citrate buffer, pH 6.0. A ready to use HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody. Counter stained with hematoxylin.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51256).

    References

    ab235388 has not yet been referenced specifically in any publications.

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