Overview

  • Product name
  • Description
    Rabbit polyclonal to WSX-1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human WSX-1 aa 44-59 (N terminal).
    Sequence:

    GSAGPLQCYGVGPLGD

  • Positive control
    • Human spleen tissue lysate.
  • General notes
    Stable for one year.

    Protein previously labeled as TCCR.

Properties

Applications

Our Abpromise guarantee covers the use of ab5997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 70 kDa.
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 10 µg/ml.

Target

  • Function
    Receptor for IL27. Requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1-type immune responses. Also appears to be involved in innate defense mechanisms.
  • Tissue specificity
    Highly expressed in lymphoid tissues such as spleen, lymph nodes and peripheral blood leukocytes. Weakly expressed in other tissues examined including heart, brain, fetal and adult lung, liver, skeletal muscle, kidney, pancreas, prostate, testis, ovary, small intestine, kidney and colon. In the lymphoid system, higher level expression in CD4+ T-cell subsets than in CD8+ T-cell subsets. Also weaker expression in CD19+ B-cells and monocytes.
  • Sequence similarities
    Belongs to the type I cytokine receptor family. Type 2 subfamily.
    Contains 3 fibronectin type-III domains.
  • Domain
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Class I cytokine receptor antibody
    • CRL1 antibody
    • Cytokine receptor-like 1 antibody
    • I27RA_HUMAN antibody
    • IL-27 receptor subunit alpha antibody
    • IL-27R subunit alpha antibody
    • IL-27R-alpha antibody
    • IL-27RA antibody
    • IL27R alpha antibody
    • IL27R antibody
    • Il27ra antibody
    • Interleukin 27 receptor alpha chain precursor antibody
    • Interleukin 27 receptor antibody
    • Interleukin-27 receptor subunit alpha antibody
    • Protein CRL1 antibody
    • T cell cytokine receptor type 1 antibody
    • TCCR antibody
    • Type 1 T cell cytokine receptor antibody
    • Type I T-cell cytokine receptor antibody
    • WSX-1 antibody
    • WSX1 antibody
    • ZcytoR1 antibody
    see all

Images

  • Western blot analysis of WSX-1 expression in human spleen tissue lysates with ab5997 at 1 µg /ml. Western blot analysis of WSX-1 expression in human spleen tissue lysates with ab5997 at 1 µg /ml.

  • ab5997 at 10µg/ml staining WSX-1 in human spleen tissue by IHC

  • Immunofluorescence of WSX-1 in A549 cells using ab5997 at 20 ug/ml.

References

This product has been referenced in:
  • Seita J  et al. Interleukin-27 directly induces differentiation in hematopoietic stem cells. Blood 111:1903-12 (2008). Read more (PubMed: 18042804) »
  • Yoshimura T  et al. Two-sided roles of IL-27: induction of Th1 differentiation on naive CD4+ T cells versus suppression of proinflammatory cytokine production including IL-23-induced IL-17 on activated CD4+ T cells partially through STAT3-dependent mechanism. J Immunol 177:5377-85 (2006). Read more (PubMed: 17015723) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us. I am sorry to hear you are having problems with one of our products. Unfortunately, I am not available to send positive controls, but I can suggest using Human spleen tissue lysate with this antibody. We have in catalogue several lysates from Human Spleen Tissue if you are interested (see ab29699 and ab29689). https://www.abcam.com/Spleen-Human-Tissue-Lysate-adult-normal-tissue-ab29699.html https://www.abcam.com/Spleen-Human-Membrane-Lysate-adult-normal-tissue-ab29689.html However, I would be very happy to help you solve the problems experienced, and if no suggestions can be made to the protocol, and the antibody was purchased within the last 6 months, it is covered by our Abpromise guarantee, and therefore I can offer you a free of charge replacement or a credit note for it. I would appreciate if you could send me further details about the sample preparation such as the lysis buffer, the incubation process, the blocking conditions, etc... I send you a questionnaire attached to this email, which only takes about 5 minutes to be filled, and it will be very helpful to us to better understand why the antibody did not perform as stated in the datasheet. I would also appreciate if you could send us an image of the membrane, to better understand the problem. I look forward to receiving your reply. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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