Product nameAnti-WSX-1 antibody [EPR20863-3] - BSA and Azide free
See all WSX-1 primary antibodies
DescriptionRabbit monoclonal [EPR20863-3] to WSX-1 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse WSX-1 aa 1-250. The exact sequence is proprietary.
Database link: O70394
- WB: Wild-type mouse spleen and bone marrow tissue lysates.
ab273155 is the carrier-free version of ab220359. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab273155 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.??
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab273155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 110, 120 kDa (predicted molecular weight: 69 kDa).|
FunctionReceptor for IL27. Requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1-type immune responses. Also appears to be involved in innate defense mechanisms.
Tissue specificityHighly expressed in lymphoid tissues such as spleen, lymph nodes and peripheral blood leukocytes. Weakly expressed in other tissues examined including heart, brain, fetal and adult lung, liver, skeletal muscle, kidney, pancreas, prostate, testis, ovary, small intestine, kidney and colon. In the lymphoid system, higher level expression in CD4+ T-cell subsets than in CD8+ T-cell subsets. Also weaker expression in CD19+ B-cells and monocytes.
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 3 fibronectin type-III domains.
DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
- Information by UniProt
- Class I cytokine receptor antibody
- CRL1 antibody
- Cytokine receptor-like 1 antibody
All lanes : Anti-WSX-1 antibody [EPR20863-3] (ab220359) at 1/1000 dilution
Lane 1 : Wild-type mouse spleen (1) tissue lysate
Lane 2 : Wild-type mouse spleen (2) tissue lysate
Lane 3 : Wild-type mouse spleen (3) tissue lysate
Lane 4 : IL27Ra knockout mouse spleen (1) tissue lysate
Lane 5 : IL27Ra knockout mouse spleen (2) tissue lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 69 kDa
Observed band size: 110/120 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Data from collaborator.
Exposure time: 1 minute.
WSX-1 = IL27Ra
These data were developed using the same antibody clone in a different buffer formulation containing PBS, glycyerol, BSA and sodium azide (ab220359).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab273155 has been referenced in 1 publication.
- He H et al. Aging-induced IL27Ra Signaling Impairs Hematopoietic Stem Cells. Blood N/A:N/A (2020). PubMed: 32305041