Anti-WTAP antibody (ab155655)

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Overview

  • Product name

    Anti-WTAP antibody
    See all WTAP primary antibodies

  • Description

    Rabbit polyclonal to WTAP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis

  • Immunogen

    Recombinant protein fragment, corresponding to a region within amino acids 1-215 of Human WTAP (UniProt ID: Q15007).

  • Positive control

    • MOLT4 cell lysate; HeLa cells; Human A549 Xenograft tissue.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 44 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is also possible using Tris-EDTA buffer (pH 8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes. May be involved in mRNA splicing regulation.

  • Tissue specificity

    Ubiquitously expressed.

  • Sequence similarities

    Belongs to the fl(2)d family.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Nucleus > nucleolus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • DKFZp686F20131 antibody
    • Female-lethal(2)D homolog antibody
    • FL2D_HUMAN antibody
    • hFL(2)D antibody
    • KIAA0105 antibody
    • MGC3925 antibody
    • Mum2 antibody
    • OTTHUMP00000017522 antibody
    • OTTHUMP00000017523 antibody
    • PNAS 132 antibody
    • PNAS-132 antibody
    • PNAS132 antibody
    • Pre mRNA splicing regulator WTAP antibody
    • Pre-mRNA-splicing regulator WTAP antibody
    • Putative pre mRNA splicing regulator female lethal 2D homolog antibody
    • Putative pre mRNA splicing regulator female lethal(2D) antibody
    • Putative pre-mRNA splicing regulator female lethal(2D) homolog antibody
    • putative pre-mRNA splicing regulator female-lethal(2D) antibody
    • Wilms tumor 1 associated protein antibody
    • Wilms tumor 1 associating protein antibody
    • Wilms tumor 1-associating protein antibody
    • Wilms' tumor 1 associating protein antibody
    • Wilms' tumour 1-associating protein antibody
    • WT1 associated protein antibody
    • WT1-associated protein antibody
    • WT1-associating protein antibody
    • wtap antibody
    see all

Images

  • Western blot - Anti-WTAP antibody (ab155655)
    Western blot - Anti-WTAP antibody (ab155655)
    Anti-WTAP antibody (ab155655) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 44 kDa



    10% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WTAP antibody (ab155655)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WTAP antibody (ab155655)
    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human A549 Xenograft tissue, labeling WTAP using ab155655 at a 1/100 dilution.
  • Immunocytochemistry/ Immunofluorescence - Anti-WTAP antibody (ab155655)
    Immunocytochemistry/ Immunofluorescence - Anti-WTAP antibody (ab155655)
    Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling WTAP using ab155655 at a 1/500 dilution (green). The slide was costained with an alpha Tubulin antibody (red) at a 1/2000 dilution.

References

This product has been referenced in:

  • Hao H  et al. N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication. Nucleic Acids Res 47:362-374 (2019). Read more (PubMed: 30364964) »
  • Kuai Y  et al. Wilms' tumor 1-associating protein plays an aggressive role in diffuse large B-cell lymphoma and forms a complex with BCL6 via Hsp90. Cell Commun Signal 16:50 (2018). Read more (PubMed: 30143009) »
See all 3 Publications for this product

Publishing research using ab155655? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Western blot abreview for Anti-WTAP antibody

 Average
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - nuclear (embryonic kidney cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
embryonic kidney cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Read More

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Verified customer

Submitted Dec 07 2018

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