Anti-WTAP antibody (ab155655)

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Overview

  • Product name
    Anti-WTAP antibody
    See all WTAP primary antibodies
  • Description
    Rabbit polyclonal to WTAP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant protein fragment, corresponding to a region within amino acids 1-215 of Human WTAP (UniProt ID: Q15007).

  • Positive control
    • MOLT4 cell lysate; HeLa cells; Human A549 Xenograft tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 44 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is also possible using Tris-EDTA buffer (pH 8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes. May be involved in mRNA splicing regulation.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the fl(2)d family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus > nucleolus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • DKFZp686F20131 antibody
    • Female-lethal(2)D homolog antibody
    • FL2D_HUMAN antibody
    • hFL(2)D antibody
    • KIAA0105 antibody
    • MGC3925 antibody
    • Mum2 antibody
    • OTTHUMP00000017522 antibody
    • OTTHUMP00000017523 antibody
    • PNAS 132 antibody
    • PNAS-132 antibody
    • PNAS132 antibody
    • Pre mRNA splicing regulator WTAP antibody
    • Pre-mRNA-splicing regulator WTAP antibody
    • Putative pre mRNA splicing regulator female lethal 2D homolog antibody
    • Putative pre mRNA splicing regulator female lethal(2D) antibody
    • Putative pre-mRNA splicing regulator female lethal(2D) homolog antibody
    • putative pre-mRNA splicing regulator female-lethal(2D) antibody
    • Wilms tumor 1 associated protein antibody
    • Wilms tumor 1 associating protein antibody
    • Wilms tumor 1-associating protein antibody
    • Wilms' tumor 1 associating protein antibody
    • Wilms' tumour 1-associating protein antibody
    • WT1 associated protein antibody
    • WT1-associated protein antibody
    • WT1-associating protein antibody
    • wtap antibody
    see all

Images

  • Western blot - Anti-WTAP antibody (ab155655)
    Western blot - Anti-WTAP antibody (ab155655)
    Anti-WTAP antibody (ab155655) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 44 kDa



    10% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WTAP antibody (ab155655)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WTAP antibody (ab155655)
    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human A549 Xenograft tissue, labeling WTAP using ab155655 at a 1/100 dilution.
  • Immunocytochemistry/ Immunofluorescence - Anti-WTAP antibody (ab155655)
    Immunocytochemistry/ Immunofluorescence - Anti-WTAP antibody (ab155655)
    Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling WTAP using ab155655 at a 1/500 dilution (green). The slide was costained with an alpha Tubulin antibody (red) at a 1/2000 dilution.

References

This product has been referenced in:
  • Kuai Y  et al. Wilms' tumor 1-associating protein plays an aggressive role in diffuse large B-cell lymphoma and forms a complex with BCL6 via Hsp90. Cell Commun Signal 16:50 (2018). Read more (PubMed: 30143009) »
  • Li BQ  et al. WT1-associated protein is a novel prognostic factor in pancreatic ductal adenocarcinoma. Oncol Lett 13:2531-2538 (2017). IHC-P ; Human . Read more (PubMed: 28454430) »
See all 2 Publications for this product

Publishing research using ab155655? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Western blot abreview for Anti-WTAP antibody

 Average
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - nuclear (embryonic kidney cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
embryonic kidney cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Read More

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Submitted Dec 07 2018

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