Anti-WTAP antibody (ab155655)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-WTAP antibody
See all WTAP primary antibodies -
DescriptionRabbit polyclonal to WTAP
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
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Immunogen
Recombinant protein fragment, corresponding to a region within amino acids 1-215 of Human WTAP (UniProt ID: Q15007).
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Positive control
- MOLT4 cell lysate; HeLa cells; Human A549 Xenograft tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab155655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 44 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is also possible using Tris-EDTA buffer (pH 8.0). | |
| ICC/IF | 1/100 - 1/1000. |
Target
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FunctionRegulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes. May be involved in mRNA splicing regulation.
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Tissue specificityUbiquitously expressed.
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Sequence similaritiesBelongs to the fl(2)d family.
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Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localizationNucleus > nucleolus.
- Information by UniProt
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Database links
- Entrez Gene: 532996 Cow
- Entrez Gene: 9589 Human
- Entrez Gene: 60532 Mouse
- Entrez Gene: 499020 Rat
- Entrez Gene: 734639 Xenopus laevis
- Entrez Gene: 334058 Zebrafish
- Omim: 605442 Human
- SwissProt: Q15007 Human
see all -
Alternative names
- DKFZp686F20131 antibody
- Female-lethal(2)D homolog antibody
- FL2D_HUMAN antibody
see all
Images
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Western blot - Anti-WTAP antibody (ab155655)Anti-WTAP antibody (ab155655) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 44 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WTAP antibody (ab155655)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human A549 Xenograft tissue, labeling WTAP using ab155655 at a 1/100 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-WTAP antibody (ab155655)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling WTAP using ab155655 at a 1/500 dilution (green). The slide was costained with an alpha Tubulin antibody (red) at a 1/2000 dilution.
Protocols
References
This product has been referenced in:
- Kuai Y et al. Wilms' tumor 1-associating protein plays an aggressive role in diffuse large B-cell lymphoma and forms a complex with BCL6 via Hsp90. Cell Commun Signal 16:50 (2018). Read more (PubMed: 30143009) »
- Li BQ et al. WT1-associated protein is a novel prognostic factor in pancreatic ductal adenocarcinoma. Oncol Lett 13:2531-2538 (2017). IHC-P ; Human . Read more (PubMed: 28454430) »
