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    wtap-antibody-ab155655.pdf

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Epigenetics and Nuclear Signaling Transcription Other factors
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Anti-WTAP antibody (ab155655)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (3)

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Western blot - Anti-WTAP antibody (ab155655)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WTAP antibody (ab155655)
  • Immunocytochemistry/ Immunofluorescence - Anti-WTAP antibody (ab155655)

Key features and details

  • Rabbit polyclonal to WTAP
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Protein
Recombinant Human WTAP protein (ab198661)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-WTAP antibody
    See all WTAP primary antibodies
  • Description

    Rabbit polyclonal to WTAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant protein fragment, corresponding to a region within amino acids 1-215 of Human WTAP (UniProt ID: Q15007).

  • Positive control

    • MOLT4 cell lysate; HeLa cells; Human A549 Xenograft tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • MOLT-4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant Human WTAP protein (ab198661)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/500 - 1/3000. Predicted molecular weight: 44 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is also possible using Tris-EDTA buffer (pH 8.0).
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 44 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is also possible using Tris-EDTA buffer (pH 8.0).
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes. May be involved in mRNA splicing regulation.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the fl(2)d family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus > nucleolus.
  • Target information above from: UniProt accession Q15007 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 532996 Cow
    • Entrez Gene: 9589 Human
    • Entrez Gene: 60532 Mouse
    • Entrez Gene: 499020 Rat
    • Entrez Gene: 734639 Xenopus laevis
    • Entrez Gene: 334058 Zebrafish
    • Omim: 605442 Human
    • SwissProt: Q15007 Human
    • SwissProt: Q9ER69 Mouse
    • SwissProt: Q4KLT6 Xenopus laevis
    • SwissProt: Q7SXL7 Zebrafish
    • Unigene: 446091 Human
    • Unigene: 275521 Mouse
    • Unigene: 7433 Xenopus laevis
    • Unigene: 6973 Zebrafish
    see all
  • Alternative names

    • DKFZp686F20131 antibody
    • Female-lethal(2)D homolog antibody
    • FL2D_HUMAN antibody
    • hFL(2)D antibody
    • KIAA0105 antibody
    • MGC3925 antibody
    • Mum2 antibody
    • OTTHUMP00000017522 antibody
    • OTTHUMP00000017523 antibody
    • PNAS 132 antibody
    • PNAS-132 antibody
    • PNAS132 antibody
    • Pre mRNA splicing regulator WTAP antibody
    • Pre-mRNA-splicing regulator WTAP antibody
    • Putative pre mRNA splicing regulator female lethal 2D homolog antibody
    • Putative pre mRNA splicing regulator female lethal(2D) antibody
    • Putative pre-mRNA splicing regulator female lethal(2D) homolog antibody
    • putative pre-mRNA splicing regulator female-lethal(2D) antibody
    • Wilms tumor 1 associated protein antibody
    • Wilms tumor 1 associating protein antibody
    • Wilms tumor 1-associating protein antibody
    • Wilms' tumor 1 associating protein antibody
    • Wilms' tumour 1-associating protein antibody
    • WT1 associated protein antibody
    • WT1-associated protein antibody
    • WT1-associating protein antibody
    • wtap antibody
    see all

Images

  • Western blot - Anti-WTAP antibody (ab155655)
    Western blot - Anti-WTAP antibody (ab155655)
    Anti-WTAP antibody (ab155655) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 44 kDa



    10% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WTAP antibody (ab155655)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WTAP antibody (ab155655)
    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human A549 Xenograft tissue, labeling WTAP using ab155655 at a 1/100 dilution.
  • Immunocytochemistry/ Immunofluorescence - Anti-WTAP antibody (ab155655)
    Immunocytochemistry/ Immunofluorescence - Anti-WTAP antibody (ab155655)
    Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling WTAP using ab155655 at a 1/500 dilution (green). The slide was costained with an alpha Tubulin antibody (red) at a 1/2000 dilution.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (3)

Publishing research using ab155655? Please let us know so that we can cite the reference in this datasheet.

ab155655 has been referenced in 3 publications.

  • Hao H  et al. N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication. Nucleic Acids Res 47:362-374 (2019). PubMed: 30364964
  • Kuai Y  et al. Wilms' tumor 1-associating protein plays an aggressive role in diffuse large B-cell lymphoma and forms a complex with BCL6 via Hsp90. Cell Commun Signal 16:50 (2018). PubMed: 30143009
  • Li BQ  et al. WT1-associated protein is a novel prognostic factor in pancreatic ductal adenocarcinoma. Oncol Lett 13:2531-2538 (2017). IHC-P ; Human . PubMed: 28454430

Customer reviews and Q&As

Show All Reviews Q&A
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Western blot abreview for Anti-WTAP antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - nuclear (embryonic kidney cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
embryonic kidney cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Read More

Abcam user community

Verified customer

Submitted Dec 07 2018

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