Anti-WTAP antibody (ab155655)
Key features and details
- Rabbit polyclonal to WTAP
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-WTAP antibody
See all WTAP primary antibodies -
Description
Rabbit polyclonal to WTAP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
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Immunogen
Recombinant protein fragment, corresponding to a region within amino acids 1-215 of Human WTAP (UniProt ID: Q15007).
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Positive control
- MOLT4 cell lysate; HeLa cells; Human A549 Xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 44 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is also possible using Tris-EDTA buffer (pH 8.0).
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| ICC/IF |
1/100 - 1/1000.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 44 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is also possible using Tris-EDTA buffer (pH 8.0). |
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ICC/IF
1/100 - 1/1000. |
Target
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Function
Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes. May be involved in mRNA splicing regulation. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the fl(2)d family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 532996 Cow
- Entrez Gene: 9589 Human
- Entrez Gene: 60532 Mouse
- Entrez Gene: 499020 Rat
- Entrez Gene: 734639 Xenopus laevis
- Entrez Gene: 334058 Zebrafish
- Omim: 605442 Human
- SwissProt: Q15007 Human
see all -
Alternative names
- DKFZp686F20131 antibody
- Female-lethal(2)D homolog antibody
- FL2D_HUMAN antibody
see all
Images
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Western blot - Anti-WTAP antibody (ab155655)Anti-WTAP antibody (ab155655) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 44 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WTAP antibody (ab155655)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human A549 Xenograft tissue, labeling WTAP using ab155655 at a 1/100 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-WTAP antibody (ab155655)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling WTAP using ab155655 at a 1/500 dilution (green). The slide was costained with an alpha Tubulin antibody (red) at a 1/2000 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab155655 has been referenced in 3 publications.
- Hao H et al. N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication. Nucleic Acids Res 47:362-374 (2019). PubMed: 30364964
- Kuai Y et al. Wilms' tumor 1-associating protein plays an aggressive role in diffuse large B-cell lymphoma and forms a complex with BCL6 via Hsp90. Cell Commun Signal 16:50 (2018). PubMed: 30143009
- Li BQ et al. WT1-associated protein is a novel prognostic factor in pancreatic ductal adenocarcinoma. Oncol Lett 13:2531-2538 (2017). IHC-P ; Human . PubMed: 28454430