Overview

  • Product name

  • Description

    Rabbit polyclonal to WWOX
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide within Human WWOX aa 75-125. The exact sequence is proprietary. (NP_057457.1)
    Database link: Q9NZC7

  • Positive control

    • IP: Jurkat whole cell lysate. IHC-P: Human breast carcinoma tissue; Mouse renal cell carcinoma tissue.
  • General notes

    ab226221 has not performed satisfactorily when used for WB of WWOX in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of WWOX.

Properties

Applications

Our Abpromise guarantee covers the use of ab226221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (By similarity). May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm.
    • Tissue specificity

      Widely expressed. Strongly expressed in testis, prostate, and ovary. Overexpressed in cancer cell lines. Isoform 5 and isoform 6 may only be expressed in tumor cell lines.
    • Involvement in disease

      Note=Defects in WWOX may be involved in several cancer types. The gene spans the second most common chromosomal fragile site (FRA16D) which is frequently altered in cancers. Alteration of the expression and expression of some isoforms is associated with cancers. However, it is still unclear if alteration of WWOX is directly implicated in cancerogenesis or if it corresponds to a secondary effect.
      Defects in WWOX may be a cause of esophageal cancer (ESCR) [MIM:133239].
    • Sequence similarities

      Belongs to the short-chain dehydrogenases/reductases (SDR) family.
      Contains 2 WW domains.
    • Domain

      The WW 1 domain mediates interaction with TP53, and probably TP73, TFAP2C, LITAF and WBP1.
    • Post-translational
      modifications

      Phosphorylated upon genotoxic stress. Phosphorylation of Tyr-33 regulates interaction with TP53, TP73 and MAPK8. May also regulate proapoptotic activity. Phosphorylation by TNK2 is associated with polyubiquitination and degradation.
      Ubiquitinated when phosphorylated by TNK2, leading to its degradation.
    • Cellular localization

      Cytoplasm. Nucleus. Mitochondrion. Golgi apparatus. Partially localizes to the mitochondria. Translocates to the nucleus upon genotoxic stress or TNF stimulation (By similarity). Translocates to the nucleus in response to TGFB1. Isoform 5 and isoform 6 may localize in the nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • 5330426P09Rik antibody
      • 9030416C10Rik antibody
      • Aberrant WW domain-containing oxidoreductase antibody
      • D16S432E antibody
      • EC 1.1.1.- antibody
      • EIEE28 antibody
      • FOR antibody
      • FRA16D antibody
      • Fragile site FRA16D oxidoreductase antibody
      • Fragile site FRA16D Oxireductase antibody
      • HHCMA56 antibody
      • MGC55975 antibody
      • PRO0128 antibody
      • Putative oxidoreductase antibody
      • SCAR12 antibody
      • SDR41C1 antibody
      • Short chain dehydrogenase/reductase family 41C, member 1 antibody
      • WOX1 antibody
      • WW domain containing oxidoreductase antibody
      • WW domain-containing oxidoreductase antibody
      • WW domain-containing protein WWOX antibody
      • wwox antibody
      • WWOX_HUMAN antibody
      • zgc:55975 antibody
      see all

    Images

    • WWOX was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226221 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226221 at 0.4 µg/ml.

      Lane 1: ab226221 IP in Jurkat whole cell lysate.

      Lane 2: Control IgG IP in Jurkat whole cell lysate.

      Detection: Chemiluminescence with exposure time of 10 seconds.

    • Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for WWOX using ab226221 at 1/1000 dilution in immunohistochemical analysis.

      Detection: DAB staining. Counterstain: Hematoxylin (blue).

    • Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for WWOX using ab226221 at 1/1000 dilution in immunohistochemical analysis.

      Detection: DAB staining.

    References

    ab226221 has not yet been referenced specifically in any publications.

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