Overview

  • Product name

    Anti-WWOX (phospho Y33) antibody
    See all WWOX primary antibodies
  • Description

    Rabbit polyclonal to WWOX (phospho Y33)
  • Host species

    Rabbit
  • Specificity

    ab193624 detects endogenous levels of WWOX only when phosphorylated at tyrosine 33.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human WWOX (phospho Y33) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9NZC7

  • Positive control

    • Extracts from HepG2 cells treated with PMA. Human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab193624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 47 kDa).
IHC-P 1/50 - 1/100.

Target

  • Function

    Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (By similarity). May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm.
  • Tissue specificity

    Widely expressed. Strongly expressed in testis, prostate, and ovary. Overexpressed in cancer cell lines. Isoform 5 and isoform 6 may only be expressed in tumor cell lines.
  • Involvement in disease

    Note=Defects in WWOX may be involved in several cancer types. The gene spans the second most common chromosomal fragile site (FRA16D) which is frequently altered in cancers. Alteration of the expression and expression of some isoforms is associated with cancers. However, it is still unclear if alteration of WWOX is directly implicated in cancerogenesis or if it corresponds to a secondary effect.
    Defects in WWOX may be a cause of esophageal cancer (ESCR) [MIM:133239].
  • Sequence similarities

    Belongs to the short-chain dehydrogenases/reductases (SDR) family.
    Contains 2 WW domains.
  • Domain

    The WW 1 domain mediates interaction with TP53, and probably TP73, TFAP2C, LITAF and WBP1.
  • Post-translational
    modifications

    Phosphorylated upon genotoxic stress. Phosphorylation of Tyr-33 regulates interaction with TP53, TP73 and MAPK8. May also regulate proapoptotic activity. Phosphorylation by TNK2 is associated with polyubiquitination and degradation.
    Ubiquitinated when phosphorylated by TNK2, leading to its degradation.
  • Cellular localization

    Cytoplasm. Nucleus. Mitochondrion. Golgi apparatus. Partially localizes to the mitochondria. Translocates to the nucleus upon genotoxic stress or TNF stimulation (By similarity). Translocates to the nucleus in response to TGFB1. Isoform 5 and isoform 6 may localize in the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5330426P09Rik antibody
    • 9030416C10Rik antibody
    • Aberrant WW domain-containing oxidoreductase antibody
    • D16S432E antibody
    • EC 1.1.1.- antibody
    • EIEE28 antibody
    • FOR antibody
    • FRA16D antibody
    • Fragile site FRA16D oxidoreductase antibody
    • Fragile site FRA16D Oxireductase antibody
    • HHCMA56 antibody
    • MGC55975 antibody
    • PRO0128 antibody
    • Putative oxidoreductase antibody
    • SCAR12 antibody
    • SDR41C1 antibody
    • Short chain dehydrogenase/reductase family 41C, member 1 antibody
    • WOX1 antibody
    • WW domain containing oxidoreductase antibody
    • WW domain-containing oxidoreductase antibody
    • WW domain-containing protein WWOX antibody
    • wwox antibody
    • WWOX_HUMAN antibody
    • zgc:55975 antibody
    see all

Images

  • All lanes : Anti-WWOX (phospho Y33) antibody (ab193624) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells treated with PMA
    Lane 2 : Extracts from HepG2 cells treated with PMA with antigen specific peptide

    Predicted band size: 47 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue sections labeling WWOX (phospho Y33) with ab193624 at a 1/50 dilution. Right image preincubated with blocking peptide.

References

ab193624 has not yet been referenced specifically in any publications.

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