Key features and details
- Rabbit polyclonal to WWOX (phospho Y33)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-WWOX (phospho Y33) antibody
See all WWOX primary antibodies
DescriptionRabbit polyclonal to WWOX (phospho Y33)
Specificityab193624 detects endogenous levels of WWOX only when phosphorylated at tyrosine 33.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human WWOX (phospho Y33) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q9NZC7
- Extracts from HepG2 cells treated with PMA. Human colon carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, 49% PBS
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab193624 was purified by affinity-chromatography using an-epitope specific phosphopeptide. Non-phosphopeptide specific antibodies were removed by chromatography using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab193624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 47 kDa).|
|IHC-P||1/50 - 1/100.|
FunctionPutative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (By similarity). May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm.
Tissue specificityWidely expressed. Strongly expressed in testis, prostate, and ovary. Overexpressed in cancer cell lines. Isoform 5 and isoform 6 may only be expressed in tumor cell lines.
Involvement in diseaseNote=Defects in WWOX may be involved in several cancer types. The gene spans the second most common chromosomal fragile site (FRA16D) which is frequently altered in cancers. Alteration of the expression and expression of some isoforms is associated with cancers. However, it is still unclear if alteration of WWOX is directly implicated in cancerogenesis or if it corresponds to a secondary effect.
Defects in WWOX may be a cause of esophageal cancer (ESCR) [MIM:133239].
Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
Contains 2 WW domains.
DomainThe WW 1 domain mediates interaction with TP53, and probably TP73, TFAP2C, LITAF and WBP1.
modificationsPhosphorylated upon genotoxic stress. Phosphorylation of Tyr-33 regulates interaction with TP53, TP73 and MAPK8. May also regulate proapoptotic activity. Phosphorylation by TNK2 is associated with polyubiquitination and degradation.
Ubiquitinated when phosphorylated by TNK2, leading to its degradation.
Cellular localizationCytoplasm. Nucleus. Mitochondrion. Golgi apparatus. Partially localizes to the mitochondria. Translocates to the nucleus upon genotoxic stress or TNF stimulation (By similarity). Translocates to the nucleus in response to TGFB1. Isoform 5 and isoform 6 may localize in the nucleus.
- Information by UniProt
- 5330426P09Rik antibody
- 9030416C10Rik antibody
- Aberrant WW domain-containing oxidoreductase antibody
All lanes : Anti-WWOX (phospho Y33) antibody (ab193624) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells treated with PMA
Lane 2 : Extracts from HepG2 cells treated with PMA with antigen specific peptide
Predicted band size: 47 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue sections labeling WWOX (phospho Y33) with ab193624 at a 1/50 dilution. Right image preincubated with blocking peptide.
ab193624 has not yet been referenced specifically in any publications.