• Product name

  • Description

    Rabbit polyclonal to WWP1
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 200 - 250 of Human WWP1 (NP_008944.1).

  • Positive control

    • HeLa whole cell lysate



Our Abpromise guarantee covers the use of ab104440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 105 kDa.
IP Use at 2-5 µg/mg of lysate.


  • Relevance

    WWP1 is an E3 ubiquitin ligase and belongs to a family of NEDD4-like proteins. WWP1 contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. The HECT domain of WWP1 has been implicated in regulating the localization and stability of p53 – inhibition of WWP1 results in a decrease in p53 expression, whilst WWP1 mediated stabilization of p53 appears to be associated with an accumulation of cytoplasmic p53. WWP1 also negatively regulates the TGF beta tumor suppressor pathway by inactivating its molecular components (SMAD2, SMAD4 and TGFbetaR1). WWP1 has been implicated in both breast and prostate cancers.
  • Cellular localization

    Cell Membrane, Cytoplasmic and Nuclear
  • Database links

  • Alternative names

    • AIP 5 antibody
    • AIP5 antibody
    • Atropin-1-interacting protein 5 antibody
    • hSDRP1 antibody
    • Nedd 4 like ubiquitin protein ligase antibody
    • nedd4 like antibody
    • NEDD4-like E3 ubiquitin-protein ligase WWP1 antibody
    • TGIF interacting ubiquitin ligase 1 antibody
    • Tiul1 antibody
    • WW domain containing E3 ubiquitin protein ligase 1 antibody
    • WW domain-containing protein 1 antibody
    • WWP 1 antibody
    see all


  • All lanes : Anti-WWP1 antibody (ab104440) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 105 kDa

    Exposure time: 30 seconds
  • Detection of Human WWP1 by Western blot of immunoprecipitates prepared from HeLa whole cell lysates (at 1 mg for IP, 20% of IP loaded) using ab104440 at 3 µg/mg lysate for immunoprecipitation and at 1 µg/ml for subsequent blotting.

    Detection by chemiluminescence with an exposure time of 10 seconds.


This product has been referenced in:

  • Yi Y  et al. Metformin Promotes AMP-activated Protein Kinase-independent Suppression of ?Np63a Protein Expression and Inhibits Cancer Cell Viability. J Biol Chem 292:5253-5261 (2017). Read more (PubMed: 28193839) »
See 1 Publication for this product

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