Key features and details
- Rabbit polyclonal to WWP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-WWP1 antibody
See all WWP1 primary antibodies
DescriptionRabbit polyclonal to WWP1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Synthetic peptide, corresponding to a region between amino acids 200 - 250 of Human WWP1 (NP_008944.1).
- HeLa whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab104440 is affinity purified using an epitope specific to WWP1 immobilized on solid support
Our Abpromise guarantee covers the use of ab104440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 105 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceWWP1 is an E3 ubiquitin ligase and belongs to a family of NEDD4-like proteins. WWP1 contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. The HECT domain of WWP1 has been implicated in regulating the localization and stability of p53 – inhibition of WWP1 results in a decrease in p53 expression, whilst WWP1 mediated stabilization of p53 appears to be associated with an accumulation of cytoplasmic p53. WWP1 also negatively regulates the TGF beta tumor suppressor pathway by inactivating its molecular components (SMAD2, SMAD4 and TGFbetaR1). WWP1 has been implicated in both breast and prostate cancers.
Cellular localizationCell Membrane, Cytoplasmic and Nuclear
- AIP 5 antibody
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All lanes : Anti-WWP1 antibody (ab104440) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 105 kDa
Exposure time: 30 seconds
Detection of Human WWP1 by Western blot of immunoprecipitates prepared from HeLa whole cell lysates (at 1 mg for IP, 20% of IP loaded) using ab104440 at 3 µg/mg lysate for immunoprecipitation and at 1 µg/ml for subsequent blotting.
Detection by chemiluminescence with an exposure time of 10 seconds.
ab104440 has been referenced in 1 publication.
- Yi Y et al. Metformin Promotes AMP-activated Protein Kinase-independent Suppression of ?Np63a Protein Expression and Inhibits Cancer Cell Viability. J Biol Chem 292:5253-5261 (2017). PubMed: 28193839