Product nameAnti-Wwp2 antibody
See all Wwp2 primary antibodies
DescriptionRabbit polyclonal to Wwp2
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
- Human kidney tissue, Jurkat cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
Our Abpromise guarantee covers the use of ab60130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 99, 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1:312500.
RelevanceWwp2 is a HECT domain containing E3 ubiqutin protein ligase. Wwp2 specifically interacts with Rpb1 and is responsible for targeting it for ubiquitination.
- AIP2 antibody
- Atrophin 1 interacting protein 2 antibody
- NEDD4 like E3 ubiquitin protein ligase antibody
Anti-Wwp2 antibody (ab60130) at 1 µg/ml + Jurkat cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/ 50,000 - 1/100,000 dilution.
Predicted band size: 99, 47 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Wwp2 with ab60130 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.
ab60130 has not yet been referenced specifically in any publications.