Key features and details
- Cell permeable chromogenic β-galactosidase substrate
- CAS Number: 7240-90-6
- Purity: > 99%
- Soluble in DMSO to 25 mM
- Form / State: Solid
- Source: Synthetic
Product nameX-Gal, chromogenic beta-galactosidase substrate
DescriptionCell permeable chromogenic β-galactosidase substrate
- 5-Bromo-4-chloro-3-indolyl beta-galactoside
Biological descriptionCell-permeable chromogenic β-galactosidase substrate. For use in blue-white recombinant bacterial colony selection and intracellular lacZ activity detection. For in vitro studies.
Storage instructionsStore at -20°C. It is important to note that this product is reported to be light sensitive. Store In the Dark. Store under desiccating conditions.
Solubility overviewSoluble in DMSO to 25 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab144388 has been referenced in 1 publication.
- Wang Y et al. ZBTB46 is a shear-sensitive transcription factor inhibiting endothelial cell proliferation via gene expression regulation of cell cycle proteins. Lab Invest 99:305-318 (2019). PubMed: 29884909