Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8971] to XAB2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
Product nameAnti-XAB2 antibody [EPR8971]
See all XAB2 primary antibodies
DescriptionRabbit monoclonal [EPR8971] to XAB2
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
Synthetic peptide within Human XAB2 aa 750-850 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9HCS7
- HeLa, K562 and HepG2 whole cell lysate (ab7900); Human breast and Human uterus tissues; HepG2 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab180181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/100.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 100 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended.
FunctionInvolved in transcription-coupled repair (TCR), transcription and pre-mRNA splicing.
Sequence similaritiesBelongs to the crooked-neck family.
Contains 14 HAT repeats.
Cellular localizationNucleus. Detected in the splicing complex carrying pre-mRNA.
- Information by UniProt
- 0610041O14Rik antibody
- adapter protein ATH 55 antibody
- Adapter protein ATH-55 antibody
All lanes : Anti-XAB2 antibody [EPR8971] (ab180181) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 100 kDa
Immunofluorescent analysis of HepG2 cells labeling XAB2 with ab180181 at 1/50.
Immunohistochemical analysis of paraffin embedded Human uterus tissue labeling XAB2 with ab180181 at 1/50.
Immunohistochemical analysis of paraffin embedded Human breast tissue labeling XAB2 with ab180181 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab180181 has not yet been referenced specifically in any publications.