Anti-XAF1 antibody (ab17204)
Key features and details
- Rabbit polyclonal to XAF1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-XAF1 antibody
See all XAF1 primary antibodies -
Description
Rabbit polyclonal to XAF1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (Human) (within the last 35 amino acids of C terminal end).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17204 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 18485100
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WB | (3) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa. A higher 50kDa band is present at times (lot dependent). This band is not blocked by the immunogen. We do not know the identity of this extra band.
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
Use at an assay dependent concentration. PubMed: 18485100 |
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa. A higher 50kDa band is present at times (lot dependent). This band is not blocked by the immunogen. We do not know the identity of this extra band. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells. -
Tissue specificity
Widely expressed. Expression is frequently down-regulated in cancer cell lines. Isoform 5 is widely expressed. Expressed in placenta (at protein level). -
Sequence similarities
Contains 1 TRAF-type zinc finger. -
Developmental stage
Selectively expressed in third trimester placenta. -
Cellular localization
Nucleus and Cytoplasm. Nucleus. Mitochondrion. Found in the cytoplasm and nucleus of placental syncytiotrophoblasts. Translocates to mitochondria upon TNF-alpha treatment. - Information by UniProt
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Database links
- Entrez Gene: 54739 Human
- Entrez Gene: 327959 Mouse
- Entrez Gene: 679600 Rat
- Omim: 606717 Human
- SwissProt: Q6GPH4 Human
- SwissProt: Q5NBU8 Mouse
- Unigene: 441975 Human
- Unigene: 291131 Mouse
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Alternative names
- BIRC 4 binding protein antibody
- BIRC4 binding protein antibody
- BIRC4-binding protein antibody
see all
Images
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Lane 1 : Anti-XAF1 antibody (ab17204) at 0.5 µg/ml
Lane 2 : Anti-XAF1 antibody (ab17204) at 1 µg/ml
Lane 3 : Anti-XAF1 antibody (ab17204) at 2 µg/ml
Lane 1 : Human spleen lysate
Lanes 2-3 : Human spleen lysate
Observed band size: 32 kDa why is the actual band size different from the predicted?Western blot analysis of XAF1 in human spleen lysate with ab17204 at
Lane A: 0.5 ug/ml
Lane B: 1ug/ml
Lane C: 2ug/ml
Higher 50kDa band presence is lot dependent. This band is not blocked by the immunogen. -
ab17204 at 2µg/ml staining XAF1 in human spleen tissue by IHC
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab17204 has been referenced in 13 publications.
- Sun G et al. WZY-321 triggers glioma cell apoptosis via XAF1 up-regulation caused by MTM-mediated miR-873 down-regulation. J Cancer 13:2312-2321 (2022). PubMed: 35517406
- Zhu C et al. SARM1 deficiency up-regulates XAF1, promotes neuronal apoptosis, and accelerates prion disease. J Exp Med 216:743-756 (2019). PubMed: 30842236
- Xu T et al. Gene Amplification-Driven Long Noncoding RNA SNHG17 Regulates Cell Proliferation and Migration in Human Non-Small-Cell Lung Cancer. Mol Ther Nucleic Acids 17:405-413 (2019). PubMed: 31310946
- Jiang X et al. Bisphenol A induced male germ cell apoptosis via IFNß-XAF1-XIAP pathway in adult mice. Toxicol Appl Pharmacol 355:247-256 (2018). PubMed: 30017639
- Jeong SI et al. XAF1 forms a positive feedback loop with IRF-1 to drive apoptotic stress response and suppress tumorigenesis. Cell Death Dis 9:806 (2018). PubMed: 30042418