Key features and details
- Rabbit polyclonal to XAF1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-XAF1 antibody
See all XAF1 primary antibodies
DescriptionRabbit polyclonal to XAF1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
A synthetic peptide corresponding to a sequence at the C-terminal of human XAF1, identical to the related rat and mouse sequence.
- WB: HepG2 cell lysate IHC-P: Human Endometrial Carcinoma Tissue ICC: MCF7 cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab81353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionSeems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells.
Tissue specificityWidely expressed. Expression is frequently down-regulated in cancer cell lines. Isoform 5 is widely expressed. Expressed in placenta (at protein level).
Sequence similaritiesContains 1 TRAF-type zinc finger.
Developmental stageSelectively expressed in third trimester placenta.
Cellular localizationNucleus and Cytoplasm. Nucleus. Mitochondrion. Found in the cytoplasm and nucleus of placental syncytiotrophoblasts. Translocates to mitochondria upon TNF-alpha treatment.
- Information by UniProt
- BIRC 4 binding protein antibody
- BIRC4 binding protein antibody
- BIRC4-binding protein antibody
ab81353 at 1µg/ml staining XAF1 in Human endometrium cancer tissue sections by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded sections). The tissue sections underwent antigen retrieval by heat. A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.
All lanes :
All lanes : HEPG2 whole cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 35 kDa
ICC/IF image of ab81353 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81353, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab81353 has been referenced in 3 publications.
- Park GB et al. Ampelopsin-induced reactive oxygen species enhance the apoptosis of colon cancer cells by activating endoplasmic reticulum stress-mediated AMPK/MAPK/XAF1 signaling. Oncol Lett 14:7947-7956 (2017). PubMed: 29250183
- Li K et al. Adenovirus encoding XAF-1 and TNF-a in the same open reading frame efficiently inhibits hepatocellular cancer cells. Mol Med Rep 13:5169-76 (2016). PubMed: 27121136
- Liu RT et al. Inflammatory mediators induced by amyloid-beta in the retina and RPE in vivo: implications for inflammasome activation in age-related macular degeneration. Invest Ophthalmol Vis Sci 54:2225-37 (2013). PubMed: 23462752