• Product name
    Xanthine Oxidase Activity Assay Kit
  • Sample type
    Cell culture supernatant, Milk, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type
    Enzyme activity
  • Sensitivity
    > 10 mU/ml
  • Range
    10 mU/ml - 1000 mU/ml
  • Assay time
    0h 40m
  • Product overview

    Xanthine Oxidase Activity Assay Kit (ab102522) is an easy and sensitive assay to determine xanthine oxidase (XO) activity in variety of samples. In the assay, XO present in the sample oxidizes xanthine to hydrogen peroxide (H2O2) which reacts stoichiometrically with OxiRed Probe to generate color (at OD = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the color or fluorescence intensity is proportional to XO content, the XO activity can be accurately measured. The kit detects 1-100 mU xanthine oxidase in 100 µl reaction volume.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Xanthine oxidase (XO, EC ) is present in appreciable amounts in the liver and jejunum in healthy individuals. However, in various liver disorders, XO is released into circulation. Therefore, determination of serum XO level serves as a sensitive indicator of acute liver damage such as jaundice.



  • Xanthine Oxidase Positive Control



This product has been referenced in:
  • Li J  et al. Artemisinins Target GABAA Receptor Signaling and Impair a Cell Identity. Cell 168:86-100.e15 (2017). Functional Studies . Read more (PubMed: 27916275) »
  • Khambata RS  et al. Antiinflammatory actions of inorganic nitrate stabilize the atherosclerotic plaque. Proc Natl Acad Sci U S A 114:E550-E559 (2017). Read more (PubMed: 28057862) »

See all 4 Publications for this product

Customer reviews and Q&As

It will be possible to prepare and use the ab102522 Xanthine Oxidase Assay Kit several times over two months.

Once you have opened the kit and prepared the components, it is highly recommended that the components be stored in aliquots at -20C....

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"Cell culture supernatants" in this instance means cell lysates. Xanthine oxidase is not released by healthy cells. To prepare cell lysates, simply pellet the cells, estimate the volume of the pellet, and add 4 volumes of the assay buffer that is provi...

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I can confirm that the kit ab102522 and ab102500 can be used to assay xanthine oxidase and Hydrogen peroxide in cells. I would suggest starting with at least 1-2 million cells. Please follow the following protocol;

2X106 cells, suspend the ce...

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En cuanto al principio de la reacción, en el “enzyme mix” no hay enzima XO. Este reactivo actúa en el segundo paso de la reacción, después de que la enzima XO de las muestras haya degradado la Xantina. El en...

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The cell pellet after removing all media can be stored at -80C with a small volume of PBS. It is not ideal to store cells with medium. Once thawed, the cells can be washed once with PBS and then the PBS can be removed. At this step the assay buffer can...

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I have double checked with the lab. Yes, the assay buffer is sufficient for efficient homogenization. You don’t need any other buffer. Just look at an aliquot of the homogenised sample under the microscope. If most cells show a broken nuclear ri...

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Thank you for your enquiry.

I am pleased to provide the following information for you:

1. Is there a preferred method to lift up the cells before centrifuging to get the supernatant/xanthine oxidase?

Cells can be trypsi...

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Thanks for your inquiry.
You can most likely use frozen tissues with limitations. We ourselves have never tried it, but I think if the tissues have been frozen efficiently and stored well, they should not pose a problem. Unfortunately we cannot gu...

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