Xanthine Oxidase Activity Assay Kit (ab102522)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture extracts, Milk, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 10 mU/ml
Overview
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Product name
Xanthine Oxidase Activity Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts -
Assay type
Enzyme activity -
Sensitivity
> 10 mU/ml -
Range
10 mU/ml - 1000 mU/ml -
Assay time
0h 40m -
Product overview
Xanthine Oxidase Activity Assay Kit ab102522 is an easy and sensitive assay to determine xanthine oxidase activity in variety of samples.
In the xanthine oxidase assay protocol, xanthine oxidase oxidizes xanthine to hydrogen peroxide (H2O2) which reacts stoichiometrically with a probe to generate color (at OD = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the color or fluorescence intensity is proportional to XO content, the XO activity can be accurately measured.
The kit detects 1-100 mU xanthine oxidase in 100 µl reaction volume.
Xanthine oxidase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader immediately and after 10-20 min -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K710 Xanthine Oxidase Activity Colorimetric/Fluorometric Assay Kit. K710-100 is the same size as the 100 test size of ab102522.
Xanthine oxidase (XO, EC 1.17.3.2 ) is present in appreciable amounts in the liver and jejunum in healthy individuals. However, in various liver disorders, XO is released into circulation. Therefore, determination of serum XO level serves as a sensitive indicator of acute liver damage such as jaundice.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer VII 1 x 25ml Developer Solution V 1 vial H2O2 Standard 1 x 0.1ml OxiRed Probe 1 x 200µl Xanthine Enzyme Mix 1 vial XO Substrate Mix 1 vial -
Research areas
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Relevance
Xanthine oxidase (XO, EC 1.17.3.2 ) is present in appreciable amounts in the liver and jejunum in healthy individuals. However, in various liver disorders, XO is released into circulation. Therefore, determination of serum XO level serves as a sensitive indicator of acute liver damage such as jaundice. -
Cellular localization
Cytoplasm. Peroxisome. Secreted. -
Alternative names
- Xanthine dehydrogenase/oxidase
- Xanthine oxidoreductase
- XO
- XOR
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab102522 has been referenced in 19 publications.
- Potue P et al. Effects of Thai native chicken breast meat consumption on serum uric acid level, biochemical parameters, and antioxidant activities in rats. Sci Rep 12:14056 (2022). PubMed: 35982129
- Shui G et al. Simiao pill inhibits epithelial mesenchymal transition in a mouse model of chronic hyperuricemic nephropathy by inhibiting NLRP3 inflammasome activation. BMC Complement Med Ther 22:278 (2022). PubMed: 36271349
- Ye H et al. The Hepatic Microenvironment Uniquely Protects Leukemia Cells through Induction of Growth and Survival Pathways Mediated by LIPG. Cancer Discov 11:500-519 (2021). PubMed: 33028621
- Vora B et al. Oxypurinol pharmacokinetics and pharmacodynamics in healthy volunteers: Influence of BCRP Q141K polymorphism and patient characteristics. Clin Transl Sci 14:1431-1443 (2021). PubMed: 33931953
- Gonzaléz-Candia A et al. Melatonin Reduces Oxidative Stress in the Right Ventricle of Newborn Sheep Gestated under Chronic Hypoxia. Antioxidants (Basel) 10:N/A (2021). PubMed: 34829529