Overview

  • Product name

    Anti-Xanthine Oxidase antibody
    See all Xanthine Oxidase primary antibodies
  • Description

    Rabbit polyclonal to Xanthine Oxidase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Cow, Human, Pig
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment corresponding to Human Xanthine Oxidase aa 1-300. N-terminal His-tag and GST-tag; Expressed in E.coli.
    Sequence:

    MTADKLVFFVNGRKVVEKNADPETTLLAYLRRKLGLSGTKLGCGEGGCGA CTVMLSKYDRLQNKIVHFSANACLAPICSLHHVAVTTVEGIGSTKTRLHP VQERIAKSHGSQCGFCTPGIVMSMYTLLRNQPEPTMEEIENAFQGNLCRC TGYRPILQGFRTFARDGGCCGGDGNNPNCCMNQKKDHSVSLSPSLFKPEE FTPLDPTQEPIFPPELLRLKDTPRKQLRFEGERVTWIQASTLKELLDLKA QHPDAKLVVGNTEIGIEMKFKNMLFPMIVCPAWIPELNSVEHGPDGISFG


    Database link: P47989

  • Positive control

    • WB: Recombinant human Xanthine Oxidase protein; Cow milk; Pig liver lysate. IHC-P: Human liver tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab233148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 2 µg/ml.
IHC-P Use a concentration of 5 - 20 µg/ml.

Target

  • Function

    Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro).
  • Tissue specificity

    Detected in milk (at protein level).
  • Involvement in disease

    Defects in XDH are the cause of xanthinuria type 1 (XU1) [MIM:278300]. Xanthinuria is characterized by excretion of very large amounts of xanthine in the urine and a tendency to form xanthine stones. Uric acid is strikingly diminished in serum and urine. XU1 is due to isolated xanthine dehydrogenase. XU1 patients can metabolize allopurinol.
  • Sequence similarities

    Belongs to the xanthine dehydrogenase family.
    Contains 1 2Fe-2S ferredoxin-type domain.
    Contains 1 FAD-binding PCMH-type domain.
  • Post-translational
    modifications

    Subject to partial proteolysis; this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).
    Contains sulfhydryl groups that are easily oxidized (in vitro); this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).
  • Cellular localization

    Cytoplasm. Peroxisome. Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Xanthine dehydrogenase antibody
    • Xanthine dehydrogenase/oxidase antibody
    • Xanthine oxidase antibody
    • Xanthine oxidoreductase antibody
    • XD antibody
    • XDH antibody
    • XDH_HUMAN antibody
    • xdha antibody
    • XO antibody
    • xor antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human liver tissue stained for Xanthine Oxidase using ab233148 at 20 µg/ml in immunohistochemical analysis. DAB staining.

  • Anti-Xanthine Oxidase antibody (ab233148) at 5 µg/ml + Pig liver lysate

    Secondary
    HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/ml
  • Anti-Xanthine Oxidase antibody (ab233148) at 5 µg/ml + Cow milk

    Secondary
    HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/ml
  • Anti-Xanthine Oxidase antibody (ab233148) at 2 µg/ml + Recombinant human Xanthine Oxidase protein

References

ab233148 has not yet been referenced specifically in any publications.

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