Anti-Xanthine Oxidase antibody (ab262927)
Key features and details
- Rabbit polyclonal to Xanthine Oxidase
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Xanthine Oxidase antibody
See all Xanthine Oxidase primary antibodies -
Description
Rabbit polyclonal to Xanthine Oxidase -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Xanthine Oxidase aa 600-750.
Database link: P47989 -
Positive control
- IHC-P: Human lactating breast tissue. ICC/IF: U-251 MG cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab262927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/5000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
Notes |
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IHC-P
1/5000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
Target
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Function
Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro). -
Tissue specificity
Detected in milk (at protein level). -
Involvement in disease
Defects in XDH are the cause of xanthinuria type 1 (XU1) [MIM:278300]. Xanthinuria is characterized by excretion of very large amounts of xanthine in the urine and a tendency to form xanthine stones. Uric acid is strikingly diminished in serum and urine. XU1 is due to isolated xanthine dehydrogenase. XU1 patients can metabolize allopurinol. -
Sequence similarities
Belongs to the xanthine dehydrogenase family.
Contains 1 2Fe-2S ferredoxin-type domain.
Contains 1 FAD-binding PCMH-type domain. -
Post-translational
modificationsSubject to partial proteolysis; this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).
Contains sulfhydryl groups that are easily oxidized (in vitro); this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O). -
Cellular localization
Cytoplasm. Peroxisome. Secreted. - Information by UniProt
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Database links
- Entrez Gene: 7498 Human
- Omim: 607633 Human
- SwissProt: P47989 Human
- Unigene: 250 Human
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Alternative names
- Xanthine dehydrogenase antibody
- Xanthine dehydrogenase/oxidase antibody
- Xanthine oxidase antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab262927 has not yet been referenced specifically in any publications.