Overview

  • Product name

    Anti-Xanthine Oxidase antibody [EPR4606]
    See all Xanthine Oxidase primary antibodies
  • Description

    Rabbit monoclonal [EPR4606] to Xanthine Oxidase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Xanthine Oxidase aa 1200-1300. The exact sequence is proprietary.

  • Positive control

    • Human fetal liver and Human milk lysates; Human liver tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 146 kDa).
IHC-P 1/50 - 1/100. Perform antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro).
    • Tissue specificity

      Detected in milk (at protein level).
    • Involvement in disease

      Defects in XDH are the cause of xanthinuria type 1 (XU1) [MIM:278300]. Xanthinuria is characterized by excretion of very large amounts of xanthine in the urine and a tendency to form xanthine stones. Uric acid is strikingly diminished in serum and urine. XU1 is due to isolated xanthine dehydrogenase. XU1 patients can metabolize allopurinol.
    • Sequence similarities

      Belongs to the xanthine dehydrogenase family.
      Contains 1 2Fe-2S ferredoxin-type domain.
      Contains 1 FAD-binding PCMH-type domain.
    • Post-translational
      modifications

      Subject to partial proteolysis; this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).
      Contains sulfhydryl groups that are easily oxidized (in vitro); this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).
    • Cellular localization

      Cytoplasm. Peroxisome. Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • Xanthine dehydrogenase antibody
      • Xanthine dehydrogenase/oxidase antibody
      • Xanthine oxidase antibody
      • Xanthine oxidoreductase antibody
      • XD antibody
      • XDH antibody
      • XDH_HUMAN antibody
      • xdha antibody
      • XO antibody
      • xor antibody
      see all

    Images

    • All lanes : Anti-Xanthine Oxidase antibody [EPR4606] (ab133268) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Human milk lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 146 kDa
      Observed band size: 150 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemical analysis of Xanthine Oxidase in paraffin embedded Human liver tissue, using ab133268 at a 1/50 dilution.

    References

    This product has been referenced in:

    • Li XQ  et al. Liquiritin suppresses UVB-induced skin injury through prevention of inflammation, oxidative stress and apoptosis through the TLR4/MyD88/NF-?B and MAPK/caspase signaling pathways. Int J Mol Med 42:1445-1459 (2018). Read more (PubMed: 29901082) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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