Overview

  • Product name
    Anti-Xanthine Oxidase antibody - N-terminal
    See all Xanthine Oxidase primary antibodies
  • Description
    Rabbit polyclonal to Xanthine Oxidase - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Xanthine Oxidase aa 213-242 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P47989

  • Positive control
    • Mouse lung tissue lysate; Human hepatocarcinoma tissue; MDA-MB231 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab176165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 146 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro).
  • Tissue specificity
    Detected in milk (at protein level).
  • Involvement in disease
    Defects in XDH are the cause of xanthinuria type 1 (XU1) [MIM:278300]. Xanthinuria is characterized by excretion of very large amounts of xanthine in the urine and a tendency to form xanthine stones. Uric acid is strikingly diminished in serum and urine. XU1 is due to isolated xanthine dehydrogenase. XU1 patients can metabolize allopurinol.
  • Sequence similarities
    Belongs to the xanthine dehydrogenase family.
    Contains 1 2Fe-2S ferredoxin-type domain.
    Contains 1 FAD-binding PCMH-type domain.
  • Post-translational
    modifications
    Subject to partial proteolysis; this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).
    Contains sulfhydryl groups that are easily oxidized (in vitro); this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).
  • Cellular localization
    Cytoplasm. Peroxisome. Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Xanthine dehydrogenase antibody
    • Xanthine dehydrogenase/oxidase antibody
    • Xanthine oxidase antibody
    • Xanthine oxidoreductase antibody
    • XD antibody
    • XDH antibody
    • XDH_HUMAN antibody
    • xdha antibody
    • XO antibody
    • xor antibody
    see all

Images

  • Anti-Xanthine Oxidase antibody - N-terminal (ab176165) at 1/1000 dilution + Mouse lung tissue lysate at 35 µg

    Predicted band size: 146 kDa

  • Flow cytometric analysis of MDA-MB231 cells labelling Xanthine Oxidase using ab176165 at 1/10 dilution (green) or negative control (blue)

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human hepatocarcinoma tissue labeling Xanthine Oxidase with ab176165 at 1/10 dilution.

References

ab176165 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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