Overview

  • Product name
    Xanthine/Hypoxanthine Assay Kit
  • Sample type
    Cell culture supernatant, Milk, Urine, Serum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells, Tissue Homogenate
  • Assay type
    Enzyme activity
  • Sensitivity
    0.4 µM
  • Assay time
    1h 00m
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Mammals
  • Product overview

    Xanthine/Hypoxanthine Assay kit (ab155900) is a product where Xanthine/Hypoxanthine is specifically oxidized by the Xanthine Enzyme Mix to form an intermediate, which reacts with Developer & Probe to form a product that can be measured colorimetrically (? = 570 nm) or fluorometrically (Ex/Em = 535/587 nm). Xanthine/Hypoxanthine Assay kit is rapid, simple and sensitive. This high-throughput suitable assay kit can detect Xanthine levels as low as 0.4 µM in various biological samples.

  • Notes

    Xanthine, a catabolic product of purine metabolism, is present in body fluids, muscle tissue and certain plants. Structurally like caffeine, Xanthine has a stimulant effect and is used clinically to treat the congestive diseases such as asthma and chronic obstructive pulmonary disease. Xanthine is metabolized into uric acid and superoxide by Xanthine oxidase. Xanthine oxidase deficiency causes the rare genetic disorder-Xanthinuria, and leads to Xanthine accumulation in urine and blood, which ultimately progresses to renal failure. Recent studies show that Xanthine levels are elevated following ischemic injury, thus Xanthine can serve as a useful marker for tissue hypoxia. Early detection of Xanthine alteration in biological fluids is crucial for metabolic studies and for diagnostic and therapeutic monitoring.

Properties

Images

  • Xanthine & Hypoxanthine measured in mouse tissue lysates by fluorimetric method showing quantity (nmol) per mg of sample tested.

  • Xanthine & Hypoxanthine measured in biological fluids by fluorimetric method showing concentrations (micromolar).

  • Xanthine & Hypoxanthine measured in mouse tissue lysates by colorimetric method showing quantity (mmol) per mg of sample tested.

Protocols

References

This product has been referenced in:
  • Brown AG  et al. Exposure to intrauterine inflammation alters metabolomic profiles in the amniotic fluid, fetal and neonatal brain in the mouse. PLoS One 12:e0186656 (2017). Read more (PubMed: 29049352) »

See 1 Publication for this product

Customer reviews and Q&As

I think more than the length of the storage, it is important that the samples are not freeze thawed multiple times.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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