Overview

  • Product name

  • Description

    Rabbit polyclonal to XBP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human XBP1.
    Database link: P17861

  • Positive control

    • HepG2 and mouse RAW264.7 cell lysates, treated with 300 nM of thapsigargin.
  • General notes

    This antibody does not recognise the unspliced isoform (XBP-1U; SwissProt# P17861-1).

Properties

Applications

Our Abpromise guarantee covers the use of ab198999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 29 kDa).

Target

  • Function

    Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I.
  • Involvement in disease

    Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature.
  • Sequence similarities

    Belongs to the bZIP family.
    Contains 1 bZIP domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Tax responsive element binding protein 5 antibody
    • Tax-responsive element-binding protein 5 antibody
    • TREB5 antibody
    • X box binding protein 1 antibody
    • X box binding protein 2 antibody
    • X-box-binding protein 1 antibody
    • XBP 1 antibody
    • XBP-1 antibody
    • XBP1 antibody
    • XBP1_HUMAN antibody
    • XBP2 antibody
    see all

Images

  • All lanes : Anti-XBP1 antibody (ab198999) at 1 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate treated with 300 nM of thapsigargin
    Lane 3 : mouse liver cell lysate
    Lane 4 : mouse brain cell lysate

    Developed using the ECL technique.

    Predicted band size: 29 kDa
    Additional bands at: 42 kDa (possible non-specific binding)

  • All lanes : Anti-XBP1 antibody (ab198999) at 1 µg/ml

    Lane 1 : mouse RAW264.7 cell lysate
    Lane 2 : mouse RAW264.7 cell lysate treated with 300 nM of thapsigargin

    Developed using the ECL technique.

    Predicted band size: 29 kDa
    Additional bands at: 42 kDa (possible non-specific binding)

References

This product has been referenced in:

  • Pandey VK  et al. Activation of PERK-eIF2a-ATF4 pathway contributes to diabetic hepatotoxicity: Attenuation of ER stress by Morin. Cell Signal 59:41-52 (2019). Read more (PubMed: 30877037) »
  • Mele L  et al. A new inhibitor of glucose-6-phosphate dehydrogenase blocks pentose phosphate pathway and suppresses malignant proliferation and metastasis in vivo. Cell Death Dis 9:572 (2018). Read more (PubMed: 29760380) »
See all 3 Publications for this product

Customer reviews and Q&As

Abreviews
Application
ChIP
Sample
Mouse Cell lysate - whole cell (Hepatocyte Cell Line)
Negative control
Ig G alone
Specification
Hepatocyte Cell Line
Detection step
Semiquantitative PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 0.75% Formaldehyde
Positive control
Tunicamycin treated cells were assayed for XBP-1 binding to the ERDJ4 promoter by ChIP. I used the abcam ChIP protocol. This antibody worked well. Lane 1: input Lane 2: IgG control Lane 3: Abcam antibody Lane 4: Competitor antibody Lane 5: PCR negative control Lane 6: 100 base pair ladder

Abcam user community

Verified customer

Submitted Sep 15 2015

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