Overview

  • Product name
  • Description
    Rabbit polyclonal to XBP1
  • Host species
    Rabbit
  • Specificity
    This product will recognize only the non-spliced (shorter) form of XBP1.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to 17 amino acids near to the C terminus of the human XBP1 protein.

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab37151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 29 kDa).Can be blocked with Human XBP1 peptide (ab39909).

Target

  • Function
    Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I.
  • Involvement in disease
    Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature.
  • Sequence similarities
    Belongs to the bZIP family.
    Contains 1 bZIP domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Tax responsive element binding protein 5 antibody
    • Tax-responsive element-binding protein 5 antibody
    • TREB5 antibody
    • X box binding protein 1 antibody
    • X box binding protein 2 antibody
    • X-box-binding protein 1 antibody
    • XBP 1 antibody
    • XBP-1 antibody
    • XBP1 antibody
    • XBP1_HUMAN antibody
    • XBP2 antibody
    see all

Images

  • Western blot analysis of XBP-1 in PC-3 cell lysate with ab37151 at (A) 0.5, (B) 1 and (C) 2 µg/ml.
  • All lanes : Anti-XBP1 antibody (ab37151) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : Human placenta tissue lysate - total protein (ab29745)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 29 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 29 kDa, 86 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    The 42 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to XBP1.

References

This product has been referenced in:
  • Pintado C  et al. Neuroinflammation alters cellular proteostasis by producing endoplasmic reticulum stress, autophagy activation and disrupting ERAD activation. Sci Rep 7:8100 (2017). Read more (PubMed: 28808322) »
  • Plaas M  et al. Wfs1- deficient rats develop primary symptoms of Wolfram syndrome: insulin-dependent diabetes, optic nerve atrophy and medullary degeneration. Sci Rep 7:10220 (2017). Read more (PubMed: 28860598) »
See all 7 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for providing that information.

As I mentioned previously, with the exception of ab79483, we do guarantee that these antibodies should work in western blot on rat tissue. If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

For ab37151, although the predicted molecular weight is 29kDa, we have some images on our webages (and attached) that show that in some samples the antibody recognizes a band of about 42kDa, which is about the size of the band that you are seeing. The difference in molecular weights could be due to pos-translational modifications, such as glycosylation etc. Therefore, I believe that this antibody is actually working well for you.

For ab50354, there is potentially a band (below the 35kDa marker) that could be the correct product, but due to the high background, it is unclear. I would advice, blocking in BSA, and then incubating the primary antibody (also in BSA) at 1/500, with overnight incubation at 4C. Hopefully that will improve the quality of the results. If not, then under the terms of our Abpromise, I will be happy to replace or refund the antibody for you.

For ab79483, as we had not tested this antibody in rat tissue, we could not guarantee that it would work for that species. I will be happy to help troubleshoot this, but it may be that the antibody is unsuitable for working in this species. You could try not only increasing the amount of total protein you are loading into the wells, but you could also increase the primary antibody concentration to 1/500.

For ab11419, again the predicted and actual molecular weights are different, but the band you are seeing just above 35kDa, may be the correct signal. A little higher than others have seen, but potentially the correct band. To confirm this, I would advise, rerunning the el, but this time let it run a little longer to get some better separation of these bands, and then as I suggested for ab50354, try blocking in BSA to help reduce the background.

Please let me know if the protocol variations do not prove to be successful, or if there is anything else that I can help you with.

Read More

Answer

Thank you for contacting Abcam.

I am sorry that you are having some issues with our antibodies. All of the antibodies you mentioned should work in western blot in various species, however only ab30315 is known to work in IHC-P. Below is a list of what applications and species we guarantee each antibody to work in:

ab37151 - ICC, WB - Mouse, Rat, Human
ab30315 - IP, WB, ICC/IF, IHC-P - Mouse, Rat, Human, Xenopus laevis
ab50354 - WB, ICC/IF - Mouse, Rat, Human
ab81959 - WB - Mouse, Rat, Human
ab79483 - WB, ELISA, IHC-P - Human
ab11419 - WB, IP, ICC/IF, IHC - Mouse, Rat, Human

To be able to help you further, would you be able to label the western blot images you sent with the molecular weights and also with what is in each lane. This way I will be able to understand the images that you sent.

Also, would you be able to answer the questions below, regarding the protocol that you are using:

1 - What sample type are you using and from what species?

2 - How much sample are you loading?

3 - What type of blocking agent are you using?

4 - What primary antibody concentrations have you tried and how long do you incubate for?

We do guarantee our antibodies to work as stated on the datasheets and if we cannot get the antibodies to work for you as we state on the datasheet, then I would be happy to replace or refund the cost of the antibody.

I look forward to your reply.

Read More

Answer

Thank you for contacting us.

The correct antibody for detection of both splice forms (as described at the UniProt page for the mouse protein, http://www.uniprot.org/uniprot/O35426 ) is ab37152:

Click here (or use the following: https://www.abcam.com/index.html?datasheet=37152).

This is raised against an N-terminal sequence that is shared by both isoforms.

Antibody ab37151 is raised against a sequence that is found only on the shorter isoform. I do not have an explanation for the result seen in the lower of the two western blots on the datasheet, the blot of HepG2 and placenta. A band at approximately 42 kDa appears but this is unlikely to be the isoform 2, long splice form of XBP1. I have asked the lab to re-test ab37151.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for confirming these details and for sending images of your results. I will forward these data to the lab, this will enable us to closely monitor the quality of our products. I am sorry the new lot ab37152 did not perform as stated on the datasheet. As requested, I have issued a free of charge replacement with one unit of ab37151; the order number is *******. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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