Anti-XBP1 antibody (ab37152)
- Datasheet
- References (49)
- Protocols
Overview
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Product nameAnti-XBP1 antibody
See all XBP1 primary antibodies -
DescriptionRabbit polyclonal to XBP1
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Host speciesRabbit
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SpecificityXBP-1 antibody will recognize both isoforms (non-spliced and spliced) of XBP-1.
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Tested applicationsSuitable for: IHC-P, IHC-Fr, WB, ICC/IF, ELISAmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide corresponding to Human XBP1 (N terminal). Synthetic peptide corresponding to 18 amino acids near to the N terminus of the human XBP1 protein. The immunogen is located within amino acids 40 - 90.
(Peptide available asab39910) -
Positive control
- Purchase matching WB positive control:Human XBP1 peptide
- HepG2 whole cell lysate (ab7900) can be used as a positive control in WB. L1210 cell lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab37152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 20028872 | |
| WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa.Can be blocked with Human XBP1 peptide (ab39910). Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa(isoform 1) and 40 kD (isoform 2). Can be blocked with XBP1 peptide (ab39910). | |
| ICC/IF | Use a concentration of 10 µg/ml. | |
| ELISA | Use at an assay dependent concentration. |
Target
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FunctionTranscription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I.
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Involvement in diseaseGenetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature.
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Sequence similaritiesBelongs to the bZIP family.
Contains 1 bZIP domain. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 7494 Human
- Entrez Gene: 22433 Mouse
- Entrez Gene: 289754 Rat
- Omim: 194355 Human
- SwissProt: P17861 Human
- SwissProt: O35426 Mouse
- SwissProt: Q9R1S4 Rat
- Unigene: 437638 Human
see all -
Alternative names
- Tax responsive element binding protein 5 antibody
- Tax-responsive element-binding protein 5 antibody
- TREB5 antibody
see all
Images
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Western blot - Anti-XBP1 antibody (ab37152)Lane 1 : Anti-XBP1 antibody (ab37152) at 1 µg/ml
Lane 2 : Anti-XBP1 antibody (ab37152) at 2 µg/ml
Lane 3 : Anti-XBP1 antibody (ab37152) at 4 µg/ml
All lanes : HepG2 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
The arrow next to the image points to the XBP1 band. -
Immunocytochemistry/ Immunofluorescence - Anti-XBP1 antibody (ab37152)Immunocytochemistry/Immunofluorescence of HepG2 cells labeling XBP1 with Anti-XBP1 antibody (ab37152) at 20μg/ml. Blue: DAPI staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)Image courtesy of an anonymous Abreview.ab37152 staining XBP1 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using Citrate-EDTA antigen retrieval buffer and microwave. Heating was performed in the microwave 3 minutes on high until boiling, 6 minutes on 20% power. Samples were then blocked for 15 minutes at 27°C followed by incubation with the primary antibody at a 1/100 dilution for 12 hours at 4°C. An undiluted HRP-conjugated rat polyclonal was used as the secondary antibody. -
Western blot - Anti-XBP1 antibody (ab37152)Lane 1 : Anti-XBP1 antibody (ab37152) at 1 µg/ml
Lane 2 : Anti-XBP1 antibody (ab37152) at 2 µg/ml
All lanes : Rat liver tissue lysate
Predicted band size: 29 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human liver tissue labeling XBP1 with Anti-XBP1 antibody (ab37152) at 5μg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)Image from Ren Yunqing et.al., PLoS Genet. 2009 June; 5(6): e1000523 (Fig 6C).ab37152 at 1/200 dilution staining XBP1 in human non-lesional skin tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraformaldehyde tissue sections). A biotin labelled secondary was used at 1/100 dilution. Diaminobenzidine (DAB) was used for staining development in a ABC staining Kit and the sections were counterstained with haematoxylin.
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Immunocytochemistry/ Immunofluorescence - Anti-XBP1 antibody (ab37152)Immunocytochemistry of XBP-1 in HepG2 cells with ab37152 at 10 ug/mL
Protocols
References
This product has been referenced in:
- Luo H et al. The Role of Tauroursodeoxycholic Acid on Dedifferentiation of Vascular Smooth Muscle Cells by Modulation of Endoplasmic Reticulum Stress and as an Oral Drug Inhibiting In-Stent Restenosis. Cardiovasc Drugs Ther 33:25-33 (2019). Read more (PubMed: 30663009) »
- Xie ZY et al. Acid-Sensing Ion Channel 1a Regulates Fate of Rat Nucleus Pulposus Cells in Acid Stimulus Through Endoplasmic Reticulum Stress. Biores Open Access 7:2-9 (2018). Read more (PubMed: 29445584) »
