Anti-XBP1 antibody (ab37152)
- Datasheet
- References (77)
- Protocols
Overview
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Product name
Anti-XBP1 antibody
See all XBP1 primary antibodies -
Description
Rabbit polyclonal to XBP1 -
Host species
Rabbit -
Specificity
XBP-1 antibody will recognize both isoforms (non-spliced and spliced) of XBP-1. -
Tested applications
Suitable for: IHC-P, IHC-Fr, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow
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Immunogen
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Positive control
- Purchase matching WB positive control:Human XBP1 peptide
- WB: HepG2 (ab7900) whole cell lysate and rat liver tissue lysate. ICC/IF: HepG2 cells. IHC-P: Human liver and human non-lesional skin tissue sections.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab37152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 20028872 | |
| WB | Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 29 kDa.Can be blocked with Human XBP1 peptide (ab39910). Human and rat samples only. Not batch tested in mouse, please see AbReviews for more information. Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa(isoform 1) and 40 kD (isoform 2). Can be blocked with XBP1 peptide (ab39910). |
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| ICC/IF | Use a concentration of 20 µg/ml. |
Target
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Function
Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I. -
Involvement in disease
Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature. -
Sequence similarities
Belongs to the bZIP family.
Contains 1 bZIP domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7494 Human
- Entrez Gene: 22433 Mouse
- Entrez Gene: 289754 Rat
- Omim: 194355 Human
- SwissProt: P17861 Human
- SwissProt: O35426 Mouse
- SwissProt: Q9R1S4 Rat
- Unigene: 437638 Human
see all -
Alternative names
- Tax responsive element binding protein 5 antibody
- Tax-responsive element-binding protein 5 antibody
- TREB5 antibody
see all
Images
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Western blot - Anti-XBP1 antibody (ab37152)Lane 1 : Anti-XBP1 antibody (ab37152) at 1 µg/ml
Lane 2 : Anti-XBP1 antibody (ab37152) at 2 µg/ml
Lane 3 : Anti-XBP1 antibody (ab37152) at 4 µg/ml
All lanes : HepG2 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?Gel: 10%
Running condition: 130V, 1.5hrs
Transfer condition: Wet, 250mA, 2 hrs (Nitrocellulose membrane)
Blocking condition: 5% non-fat milk in TBS at 4 overnight
Primary antibody incubation: Room temperature for 1 hour
Secondary antibody incubation: Room temperature for 1 hour
Washing condition: 5 mL TBST, 4 x 5 minutes
Exposure system: ECL
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Immunocytochemistry/ Immunofluorescence - Anti-XBP1 antibody (ab37152)Immunocytochemistry/Immunofluorescence of HepG2 cells labeling XBP1 with Anti-XBP1 antibody (ab37152) at 20μg/ml. Blue: DAPI staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human liver tissue labeling XBP1 with Anti-XBP1 antibody (ab37152) at 5μg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)Image courtesy of an anonymous Abreview.ab37152 staining XBP1 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using Citrate-EDTA antigen retrieval buffer and microwave. Heating was performed in the microwave 3 minutes on high until boiling, 6 minutes on 20% power. Samples were then blocked for 15 minutes at 27°C followed by incubation with the primary antibody at a 1/100 dilution for 12 hours at 4°C. An undiluted HRP-conjugated rat polyclonal was used as the secondary antibody. -
Western blot - Anti-XBP1 antibody (ab37152)Lane 1 : Anti-XBP1 antibody (ab37152) at 1 µg/ml
Lane 2 : Anti-XBP1 antibody (ab37152) at 2 µg/ml
All lanes : Rat liver tissue lysate
Predicted band size: 29 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)Image from Ren Yunqing et.al., PLoS Genet. 2009 June; 5(6): e1000523 (Fig 6C).ab37152 at 1/200 dilution staining XBP1 in human non-lesional skin tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraformaldehyde tissue sections). A biotin labelled secondary was used at 1/100 dilution. Diaminobenzidine (DAB) was used for staining development in a ABC staining Kit and the sections were counterstained with haematoxylin.
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Immunocytochemistry/ Immunofluorescence - Anti-XBP1 antibody (ab37152)Immunocytochemistry of XBP-1 in HepG2 cells with ab37152 at 10 ug/mL
Protocols
References
This product has been referenced in:
- Jiang L et al. Inhibition of microRNA-103 attenuates inflammation and endoplasmic reticulum stress in atherosclerosis through disrupting the PTEN-mediated MAPK signaling. J Cell Physiol 235:380-393 (2020). Read more (PubMed: 31232476) »
- Ma Y et al. Activation of G0/G1 switch gene 2 by endoplasmic reticulum stress enhances hepatic steatosis. Metabolism 99:32-44 (2019). Read more (PubMed: 31271806) »
