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    xbp1-antibody-ab37152.pdf

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Epigenetics and Nuclear Signaling Transcription Other factors
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Anti-XBP1 antibody (ab37152)

  • Datasheet
  • SDS
Reviews (27)Q&A (7)References (98)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)
  • Immunohistochemistry (Frozen sections) - Anti-XBP1 antibody (ab37152)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)
  • Immunohistochemistry (Frozen sections) - Anti-XBP1 antibody (ab37152)
  • Western blot - Anti-XBP1 antibody (ab37152)
  • Immunohistochemistry (Frozen sections) - Anti-XBP1 antibody (ab37152)
  • Western blot - Anti-XBP1 antibody (ab37152)
  • Immunocytochemistry/ Immunofluorescence - Anti-XBP1 antibody (ab37152)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)
  • Immunocytochemistry/ Immunofluorescence - Anti-XBP1 antibody (ab37152)

Key features and details

  • Rabbit polyclonal to XBP1
  • Suitable for: IHC-P, IHC-Fr, WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-XBP1 antibody [143F] (ab241571)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-XBP1 antibody
    See all XBP1 primary antibodies
  • Description

    Rabbit polyclonal to XBP1
  • Host species

    Rabbit
  • Specificity

    XBP-1 antibody will recognize both isoforms (non-spliced and spliced) of XBP-1.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-Fr
    Mouse
    Human
    IHC-P
    Mouse
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human XBP1 (N terminal). Synthetic peptide corresponding to 18 amino acids near to the N terminus of the human XBP1 protein. The immunogen is located within amino acids 40 - 90.
    Database link: P17861
    (Peptide available as ab39910)

  • Positive control

    • Purchase matching WB positive control:Human XBP1 peptide
    • WB: HepG2 cell lysate. IHC-P: Human liver and non-lesional skin tissue; Mouse testis and spleen tissue. IHC-Fr: Human liver and pancreas tissue; Mouse pancreas tissue. ICC: HepG2 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human XBP1 peptide (ab39910)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab37152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-Fr
Mouse
Human
IHC-P
Mouse
Human
WB
Human
All applications
Rat
Cow
Application Abreviews Notes
IHC-P (12)
Use a concentration of 5 µg/ml.
IHC-Fr
Use at an assay dependent concentration.
WB (11)
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 29 kDa.Can be blocked with Human XBP1 peptide (ab39910).

Human samples only. Not batch tested in mouse or rat, please see AbReviews for more information.

Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa (isoform 1) and 40 kD (isoform 2). Can be blocked with XBP1 peptide (ab39910).

ICC/IF (2)
Use a concentration of 20 µg/ml.
Notes
IHC-P
Use a concentration of 5 µg/ml.
IHC-Fr
Use at an assay dependent concentration.
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 29 kDa.Can be blocked with Human XBP1 peptide (ab39910).

Human samples only. Not batch tested in mouse or rat, please see AbReviews for more information.

Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa (isoform 1) and 40 kD (isoform 2). Can be blocked with XBP1 peptide (ab39910).

ICC/IF
Use a concentration of 20 µg/ml.

Target

  • Function

    Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I.
  • Involvement in disease

    Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature.
  • Sequence similarities

    Belongs to the bZIP family.
    Contains 1 bZIP domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P17861 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7494 Human
    • Entrez Gene: 22433 Mouse
    • Entrez Gene: 289754 Rat
    • Omim: 194355 Human
    • SwissProt: P17861 Human
    • SwissProt: O35426 Mouse
    • SwissProt: Q9R1S4 Rat
    • Unigene: 437638 Human
    • Unigene: 469937 Mouse
    • Unigene: 101044 Rat
    see all
  • Alternative names

    • Tax responsive element binding protein 5 antibody
    • Tax-responsive element-binding protein 5 antibody
    • TREB5 antibody
    • X box binding protein 1 antibody
    • X box binding protein 2 antibody
    • X-box-binding protein 1 antibody
    • XBP 1 antibody
    • XBP-1 antibody
    • XBP1 antibody
    • XBP1_HUMAN antibody
    • XBP2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)

    Immunohistochemistry of mouse testis cells labeling XBP1 with Anti-XBP1 antibody (ab37152) at 2μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4oC. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

  • Immunohistochemistry (Frozen sections) - Anti-XBP1 antibody (ab37152)
    Immunohistochemistry (Frozen sections) - Anti-XBP1 antibody (ab37152)

    Immunohistochemistry of human liver cells labeling XBP1 with Anti-XBP1 antibody (ab37152) at 20μg/ml. followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green). DAPI staining (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)

    Immunohistochemistry of mouse spleen cells labeling XBP1 with Anti-XBP1 antibody (ab37152) at 2μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4oC. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

  • Immunohistochemistry (Frozen sections) - Anti-XBP1 antibody (ab37152)
    Immunohistochemistry (Frozen sections) - Anti-XBP1 antibody (ab37152)

    Immunohistochemistry of mouse pancreas cells labeling XBP1 with Anti-XBP1 antibody (ab37152) at 20μg/ml. followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green). DAPI staining (Blue).

  • Western blot - Anti-XBP1 antibody (ab37152)
    Western blot - Anti-XBP1 antibody (ab37152)
    Lane 1 : Anti-XBP1 antibody (ab37152) at 1 µg/ml
    Lane 2 : Anti-XBP1 antibody (ab37152) at 2 µg/ml

    All lanes : HepG2 cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

    Predicted band size: 29 kDa

  • Immunohistochemistry (Frozen sections) - Anti-XBP1 antibody (ab37152)
    Immunohistochemistry (Frozen sections) - Anti-XBP1 antibody (ab37152)

    Immunohistochemistry of human pancreas cells labeling XBP1 with Anti-XBP1 antibody (ab37152) at 20μg/ml. followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green). DAPI staining (Blue).

  • Western blot - Anti-XBP1 antibody (ab37152)
    Western blot - Anti-XBP1 antibody (ab37152)
    Lane 1 : Anti-XBP1 antibody (ab37152) at 1 µg/ml
    Lane 2 : Anti-XBP1 antibody (ab37152) at 2 µg/ml
    Lane 3 : Anti-XBP1 antibody (ab37152) at 4 µg/ml

    All lanes : HepG2 cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/10000 dilution

    Predicted band size: 29 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?



    Gel: 10%

    Running condition: 130V, 1.5hrs

    Transfer condition: Wet, 250mA, 2 hrs (Nitrocellulose membrane)

    Blocking condition: 5% non-fat milk in TBS at 4 overnight

    Primary antibody incubation: Room temperature for 1 hour

    Secondary antibody incubation: Room temperature for 1 hour

    Washing condition: 5 mL TBST, 4 x 5 minutes

    Exposure system: ECL 

  • Immunocytochemistry/ Immunofluorescence - Anti-XBP1 antibody (ab37152)
    Immunocytochemistry/ Immunofluorescence - Anti-XBP1 antibody (ab37152)

    Immunocytochemistry/Immunofluorescence of HepG2 cells labeling XBP1 with Anti-XBP1 antibody (ab37152) at 20μg/ml. Blue: DAPI staining.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)
    Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human liver tissue labeling XBP1 with Anti-XBP1 antibody (ab37152) at 5μg/ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)Image courtesy of an anonymous Abreview.
    ab37152 staining XBP1 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using Citrate-EDTA antigen retrieval buffer and microwave. Heating was performed in the microwave 3 minutes on high until boiling, 6 minutes on 20% power. Samples were then blocked for 15 minutes at 27°C followed by incubation with the primary antibody at a 1/100 dilution for 12 hours at 4°C. An undiluted HRP-conjugated rat polyclonal was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)Image from Ren Yunqing et.al., PLoS Genet. 2009 June; 5(6): e1000523 (Fig 6C).

    ab37152 at 1/200 dilution staining XBP1 in human non-lesional skin tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraformaldehyde tissue sections). A biotin labelled secondary was used at 1/100 dilution. Diaminobenzidine (DAB) was used for staining development in a ABC staining Kit and the sections were counterstained with haematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-XBP1 antibody (ab37152)
    Immunocytochemistry/ Immunofluorescence - Anti-XBP1 antibody (ab37152)
    Immunocytochemistry of XBP-1 in HepG2 cells with ab37152 at 10 ug/mL

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (98)

    Publishing research using ab37152? Please let us know so that we can cite the reference in this datasheet.

    ab37152 has been referenced in 98 publications.

    • Jiang L  et al. Inhibition of microRNA-103 attenuates inflammation and endoplasmic reticulum stress in atherosclerosis through disrupting the PTEN-mediated MAPK signaling. J Cell Physiol 235:380-393 (2020). PubMed: 31232476
    • Hao Y  et al. 2-Methylquinazoline derivative 23BB as a highly selective histone deacetylase 6 inhibitor alleviated cisplatin-induced acute kidney injury. Biosci Rep 40:N/A (2020). PubMed: 31894849
    • Reddy SS  et al. Effect of vitamin B12 supplementation on retinal lesions in diabetic rats. Mol Vis 26:311-325 (2020). PubMed: 32355441
    • Sahin E  et al. Silymarin attenuated nonalcoholic fatty liver disease through the regulation of endoplasmic reticulum stress proteins GRP78 and XBP-1 in mice. J Food Biochem 44:e13194 (2020). PubMed: 32189355
    • Xiang L  et al. Expression of XBP1s in B lymphocytes is critical for pristane-induced lupus nephritis in mice. Am J Physiol Renal Physiol 318:F1258-F1270 (2020). PubMed: 32249615
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 34 Abreviews or Q&A

    Western blot abreview for Anti-XBP1 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (heart)
    Gel Running Conditions
    Non-reduced Denaturing (12%)
    Loading amount
    30 µg
    Specification
    heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 25 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-XBP1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Liver
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 16 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-XBP1 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Liver
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 16 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-XBP1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Liver
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 16 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-XBP1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Liver
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 16 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-XBP1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Kidney H9 Organoids)
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney H9 Organoids
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 16 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-XBP1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Liver
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 16 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-XBP1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Liver
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 16 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-XBP1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Liver
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 16 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-XBP1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (E18 embryo liver and skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    E18 embryo liver and skin
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Aug 26 2019

    1-10 of 34 Abreviews or Q&A

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