Overview

  • Product name
  • Description
    Rabbit polyclonal to XBP1
  • Host species
    Rabbit
  • Specificity
    XBP-1 antibody will recognize both isoforms (non-spliced and spliced) of XBP-1.
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Human XBP1 (N terminal). Synthetic peptide corresponding to 18 amino acids near to the N terminus of the human XBP1 protein. The immunogen is located within amino acids 40 - 90.
    Database link: P17861
    (Peptide available as ab39910)

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab37152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 20028872
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 29 kDa.Can be blocked with Human XBP1 peptide (ab39910).

Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa(isoform 1) and 40 kD (isoform 2). Can be blocked with XBP1 peptide (ab39910).

ICC/IF Use a concentration of 20 µg/ml.

Target

  • Function
    Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I.
  • Involvement in disease
    Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature.
  • Sequence similarities
    Belongs to the bZIP family.
    Contains 1 bZIP domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Tax responsive element binding protein 5 antibody
    • Tax-responsive element-binding protein 5 antibody
    • TREB5 antibody
    • X box binding protein 1 antibody
    • X box binding protein 2 antibody
    • X-box-binding protein 1 antibody
    • XBP 1 antibody
    • XBP-1 antibody
    • XBP1 antibody
    • XBP1_HUMAN antibody
    • XBP2 antibody
    see all

Images

  • Lane 1 : Anti-XBP1 antibody (ab37152) at 1 µg/ml
    Lane 2 : Anti-XBP1 antibody (ab37152) at 2 µg/ml
    Lane 3 : Anti-XBP1 antibody (ab37152) at 4 µg/ml

    All lanes : HepG2 cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/10000 dilution

    Predicted band size: 29 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?



    The arrow next to the image points to the XBP1 band.
  • Immunocytochemistry/Immunofluorescence of HepG2 cells labeling XBP1 with Anti-XBP1 antibody (ab37152) at 20μg/ml. Blue: DAPI staining.

     

  • ab37152 staining XBP1 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using Citrate-EDTA antigen retrieval buffer and microwave. Heating was performed in the microwave 3 minutes on high until boiling, 6 minutes on 20% power. Samples were then blocked for 15 minutes at 27°C followed by incubation with the primary antibody at a 1/100 dilution for 12 hours at 4°C. An undiluted HRP-conjugated rat polyclonal was used as the secondary antibody.

    See Abreview

  • Lane 1 : Anti-XBP1 antibody (ab37152) at 1 µg/ml
    Lane 2 : Anti-XBP1 antibody (ab37152) at 2 µg/ml

    All lanes : Rat liver tissue lysate

    Predicted band size: 29 kDa

  • Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human liver tissue labeling XBP1 with Anti-XBP1 antibody (ab37152) at 5μg/ml.
  • ab37152 at 1/200 dilution staining XBP1 in human non-lesional skin tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraformaldehyde tissue sections). A biotin labelled secondary was used at 1/100 dilution. Diaminobenzidine (DAB) was used for staining development in a ABC staining Kit and the sections were counterstained with haematoxylin.

  • Immunocytochemistry of XBP-1 in HepG2 cells with ab37152 at 10 ug/mL

References

This product has been referenced in:
  • Luo H  et al. The Role of Tauroursodeoxycholic Acid on Dedifferentiation of Vascular Smooth Muscle Cells by Modulation of Endoplasmic Reticulum Stress and as an Oral Drug Inhibiting In-Stent Restenosis. Cardiovasc Drugs Ther 33:25-33 (2019). Read more (PubMed: 30663009) »
  • Liu P  et al. Crizotinib-induced immunogenic cell death in non-small cell lung cancer. Nat Commun 10:1486 (2019). Read more (PubMed: 30940805) »
See all 50 Publications for this product

Customer reviews and Q&As

Filter by Application

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1-10 of 16 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
10% formalin

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Verified customer

Submitted Oct 01 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
tumor
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
10% normal buffered formalin

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Verified customer

Submitted Oct 01 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
10% Normal buffered formalin

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Verified customer

Submitted Oct 01 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
pancreas
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
10% Normal buffered formalin

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Verified customer

Submitted Oct 01 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (HaCaT cells)
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
HaCaT cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

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Verified customer

Submitted Aug 02 2018

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse neuroblastoma cell-line, Neuro2a)
Gel Running Conditions
Reduced Denaturing
Loading amount
35 µg
Treatment
0.1 uM Tg and/or 10 uM MG132 for 12 h
Specification
mouse neuroblastoma cell-line, Neuro2a
Blocking step
Milk as blocking agent for 1 hour(s) and 15 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted Oct 23 2017

Application
Western blot
Sample
Sheep Tissue lysate - whole (Small intestine whole tissue lysate)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
15 µg
Specification
Small intestine whole tissue lysate
Blocking step
Licor block buffer (PBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 37°C

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Submitted May 01 2017

Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

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Submitted Mar 16 2016

Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

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Verified customer

Submitted Mar 15 2016

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (hepatocytes)
Specification
hepatocytes
Treatment
100 ng/ml LPS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

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Verified customer

Submitted Jul 04 2014

1-10 of 16 Abreviews

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