Overview

  • Product name

  • Description

    Rabbit polyclonal to xCT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    This information is considered to be commercially sensitive.

  • Positive control

    • WB: A549, HCT 116 and HeLa whole cell lysates. ICC/IF: PC-3 cells. Flow Cytometry: Human breast carcinoma cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab216876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 55 kDa.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml.

Target

  • Function

    Sodium-independent, high-affinity exchange of anionic amino acids with high specificity for anionic form of cystine and glutamate.
  • Sequence similarities

    Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Form

    xCT has a predicted molecular weight of 55 kDa; however it has a high number of hydrophobic residues which may affect the migration of the protein in SDS-PAGE. Endogenous monomeric xCT is expected to migrate at ~35 kDa and modified-xCT is expected to migrate at ~55 kDa (PMID: 17035536)
  • Alternative names

    • Amino acid transport system xc xCT antibody antibody
    • Amino acid transport system xc- antibody
    • Calcium channel blocker resistance protein CCBR1 antibody
    • Calcium channel blocker resistance protein CCBR1 antibody antibody
    • CCBR1 antibody
    • Cysteine/glutamate transporter antibody antibody
    • Cystine/glutamate transporter antibody
    • OTTHUMP00000164578 antibody
    • SLC7A11 antibody
    • Solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11 antibody
    • solute carrier family 7 antibody
    • Solute carrier family 7 member 11 antibody
    • Solute carrier family 7, (cationic amino acid transporter, y+ system) member 11 antibody
    • SYSTEM Xc(-) TRANSPORTER-RELATED PROTEIN antibody
    • xCT antibody
    • XCT_HUMAN antibody
    see all

Images

  • All lanes : Anti-xCT antibody (ab216876) at 1/1000 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : anti-rabbit secondary DyLight 488 antibody at 1/20000 dilution

    Predicted band size: 55 kDa
    Observed band size: 56 kDa
    why is the actual band size different from the predicted?



    Dilution buffer: 0.01% sodium azide in PBS.

    xCT processing caused by dimerization, glycosylation, and/or phosphorylation may cause other bands.

  • Immunofluorescent analysis of PC-3 (human prostate adenocarcinoma cell line) cells labeling xCT with ab216876  at 10 µg/mL for 1 hour, followed by anti-Rabbit IgG DyLight™ 488 Conjugated Preadsorbed secondary antibody at 5 µg/mL for 1 hour at RT (green). Cells were fixed with 100% methanol.

    The nuclear counter stain is DAPI (blue).

    A: DAPI

    B: Primary followed by Anti-rabbit IgG Dylight™ 488

    C: Merged images

    D: Secondary only control

  • Flow cytometric analysis of human breast carcinoma cells labeling xCT with ab216876 at 1 µg/mL for 1 hour at 4°C. Rabbit IgG Antibody Dylight™488 Conjugated pre-adsorbed secondary antibody was used at 1 µg/mL for one hour on ice.

    Left panels: Unstained.

    Middle panels: Secondary antibody only.

    Right panels: Primary and secondary antibodies.

  • All lanes : Anti-xCT antibody (ab216876) at 1/1000 dilution

    Lane 1 : Recombinant Bcl3 (unrelated negative control)
    Lane 2 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate
    Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : anti-rabbit secondary HRP antibody at 1/70000 dilution

    Predicted band size: 55 kDa
    Observed band size: 56 kDa why is the actual band size different from the predicted?



    Exposure time: 15-30 seconds.

    Blocking buffer: 5% BSA.

    Dilution buffer: 0.01% sodium azide in PBS.

    xCT processing caused by dimerization, glycosylation, and/or phosphorylation may cause other bands.

References

ab216876 has not yet been referenced specifically in any publications.

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