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Signal Transduction Metabolism Amino Acids
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RecombinantRabMAb

Recombinant Anti-xCT antibody [EPR8290(2)] (ab175186)

  • Datasheet
Reviews (4) Submit a question References (20)

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Western blot - Anti-xCT antibody [EPR8290(2)] (ab175186)
  • Immunoprecipitation - Anti-xCT antibody [EPR8290(2)] (ab175186)
  • Western blot - Anti-xCT antibody [EPR8290(2)] (ab175186)
  • Immunoprecipitation - Anti-xCT antibody [EPR8290(2)] (ab175186)
  • Anti-xCT antibody [EPR8290(2)] (ab175186)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8290(2)] to xCT
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-xCT antibody [EPR8290(2)]
    See all xCT primary antibodies
  • Description

    Rabbit monoclonal [EPR8290(2)] to xCT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human xCT aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9UPY5
    (Peptide available as ab178606)

  • Positive control

    • WB: A549, HepG2, U87-MG and BxPC-3 cell lysates. Human, at and mouse heart tissue lysates IP: HepG2 cells lysate.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8290(2)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress

Associated products

  • Alternative Versions

    • Anti-xCT antibody [EPR8290(2)] - BSA and Azide free (ab238969)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HepG2 whole cell lysate (ab166833)
    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant Human xCT protein (ab112403)
  • Related Products

    • Recombinant Human xCT protein (ab112403)

Applications

Our Abpromise guarantee covers the use of ab175186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Sodium-independent, high-affinity exchange of anionic amino acids with high specificity for anionic form of cystine and glutamate.
    • Sequence similarities

      Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession Q9UPY5 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 23657 Human
      • Entrez Gene: 26570 Mouse
      • Entrez Gene: 310392 Rat
      • Omim: 607933 Human
      • SwissProt: Q9UPY5 Human
      • SwissProt: Q9WTR6 Mouse
      • Unigene: 390594 Human
      • Unigene: 260988 Mouse
      • Unigene: 28160 Rat
      see all
    • Form

      xCT has a predicted molecular weight of 55 kDa; however it has a high number of hydrophobic residues which may affect the migration of the protein in SDS-PAGE. Endogenous monomeric xCT is expected to migrate at ~35 kDa and modified-xCT is expected to migrate at ~55 kDa (PMID: 17035536)
    • Alternative names

      • Amino acid transport system xc xCT antibody antibody
      • Amino acid transport system xc- antibody
      • Calcium channel blocker resistance protein CCBR1 antibody
      • Calcium channel blocker resistance protein CCBR1 antibody antibody
      • CCBR1 antibody
      • Cysteine/glutamate transporter antibody antibody
      • Cystine/glutamate transporter antibody
      • OTTHUMP00000164578 antibody
      • SLC7A11 antibody
      • Solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11 antibody
      • solute carrier family 7 antibody
      • Solute carrier family 7 member 11 antibody
      • Solute carrier family 7, (cationic amino acid transporter, y+ system) member 11 antibody
      • SYSTEM Xc(-) TRANSPORTER-RELATED PROTEIN antibody
      • xCT antibody
      • XCT_HUMAN antibody
      see all

    Images

    • Western blot - Anti-xCT antibody [EPR8290(2)] (ab175186)
      Western blot - Anti-xCT antibody [EPR8290(2)] (ab175186)
      All lanes : Anti-xCT antibody [EPR8290(2)] (ab175186) at 1/10000 dilution (Purified)

      Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
      Lane 2 : Human heart lysates
      Lane 3 : Mouse heart lysates
      Lane 4 : Rat heart lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 55 kDa
      Observed band size: 55 kDa

    • Immunoprecipitation - Anti-xCT antibody [EPR8290(2)] (ab175186)
      Immunoprecipitation - Anti-xCT antibody [EPR8290(2)] (ab175186)

      ab175186 (purified) at 1:20 dilution (0.5µg) immunoprecipitating xCT in HepG2 whole cell lysate.
      Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab175186 & HepG2 whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab175186 in HepG2 whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Western blot - Anti-xCT antibody [EPR8290(2)] (ab175186)
      Western blot - Anti-xCT antibody [EPR8290(2)] (ab175186)
      All lanes : Anti-xCT antibody [EPR8290(2)] (ab175186) at 1/1000 dilution

      Lane 1 : A549 (Human lung carcinoma cell line) cell lysates
      Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysates
      Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysates
      Lane 4 : BxPC-3 (Human pancreas adenocarcinoma cell line) cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 55 kDa



      This image was generated using the unpurified version of the product. 

    • Immunoprecipitation - Anti-xCT antibody [EPR8290(2)] (ab175186)
      Immunoprecipitation - Anti-xCT antibody [EPR8290(2)] (ab175186)

      Western blot analysis on immunoprecipitation pellet from (Lane 1) HepG2 cells lysate or (Lane 2) 1 X PBS (negative control) using ab175186 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

      This image was generated using the unpurified version of the product. 

    • Anti-xCT antibody [EPR8290(2)] (ab175186)
      Anti-xCT antibody [EPR8290(2)] (ab175186)

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (20)

    Publishing research using ab175186? Please let us know so that we can cite the reference in this datasheet.

    ab175186 has been referenced in 20 publications.

    • Li Y  et al. Erastin/sorafenib induces cisplatin-resistant non-small cell lung cancer cell ferroptosis through inhibition of the Nrf2/xCT pathway. Oncol Lett 19:323-333 (2020). PubMed: 31897145
    • Charles-Messance H  et al. IL-1ß induces rod degeneration through the disruption of retinal glutamate homeostasis. J Neuroinflammation 17:1 (2020). PubMed: 31900165
    • Zhang H  et al. miR-30-5p-mediated ferroptosis of trophoblasts is implicated in the pathogenesis of preeclampsia. Redox Biol 29:101402 (2020). PubMed: 31926626
    • Wang W  et al. Allelic-Specific Regulation of xCT Expression Increases Susceptibility to Tuberculosis by Modulating microRNA-mRNA Interactions. mSphere 5:N/A (2020). PubMed: 32321821
    • Zhang Y  et al. Neuroprotective effect of deferoxamine on erastininduced ferroptosis in primary cortical neurons. Neural Regen Res 15:1539-1545 (2020). PubMed: 31997820
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-xCT antibody [EPR8290(2)]

    Good
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (lung)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Specification
    lung
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 21 2021

    Western blot abreview for Anti-xCT antibody [EPR8290(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (lung)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (Gel 9%)
    Loading amount
    40 µg
    Specification
    lung
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 21 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-xCT antibody [EPR8290(2)]

    Good
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
    Permeabilization
    No
    Specification
    Spleen
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 07 2018

    Western blot abreview for Anti-xCT antibody [EPR8290(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Brain tissue)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    8 µg
    Specification
    Brain tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 25 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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