Recombinant
RabMAb

Recombinant Anti-xCT antibody [EPR8290(2)] (ab175186)

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Overview

  • Product name

    Anti-xCT antibody [EPR8290(2)]
    See all xCT primary antibodies

  • Description

    Rabbit monoclonal [EPR8290(2)] to xCT

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human xCT aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9UPY5
    (Peptide available as ab178606)

  • Positive control

    • WB: A549, HepG2, U87-MG and BxPC-3 cell lysates. Human, at and mouse heart tissue lysates IP: HepG2 cells lysate.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab175186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 55 kDa.Can be blocked with xCT peptide (ab178606).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

    • Target

    • Function

      Sodium-independent, high-affinity exchange of anionic amino acids with high specificity for anionic form of cystine and glutamate.

    • Sequence similarities

      Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      see all

    • Form

      xCT has a predicted molecular weight of 55 kDa; however it has a high number of hydrophobic residues which may affect the migration of the protein in SDS-PAGE. Endogenous monomeric xCT is expected to migrate at ~35 kDa and modified-xCT is expected to migrate at ~55 kDa (PMID: 17035536)

    • Alternative names

      • Amino acid transport system xc xCT antibody antibody
      • Amino acid transport system xc- antibody
      • Calcium channel blocker resistance protein CCBR1 antibody
      • Calcium channel blocker resistance protein CCBR1 antibody antibody
      • CCBR1 antibody
      • Cysteine/glutamate transporter antibody antibody
      • Cystine/glutamate transporter antibody
      • OTTHUMP00000164578 antibody
      • SLC7A11 antibody
      • Solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11 antibody
      • solute carrier family 7 antibody
      • Solute carrier family 7 member 11 antibody
      • Solute carrier family 7, (cationic amino acid transporter, y+ system) member 11 antibody
      • SYSTEM Xc(-) TRANSPORTER-RELATED PROTEIN antibody
      • xCT antibody
      • XCT_HUMAN antibody
      see all

    Images

    • Western blot - Anti-xCT antibody [EPR8290(2)] (ab175186)
      Western blot - Anti-xCT antibody [EPR8290(2)] (ab175186)
      All lanes : Anti-xCT antibody [EPR8290(2)] (ab175186) at 1/10000 dilution (Purified)

      Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
      Lane 2 : Human heart lysates
      Lane 3 : Mouse heart lysates
      Lane 4 : Rat heart lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 55 kDa
      Observed band size: 55 kDa

    • Immunoprecipitation - Anti-xCT antibody [EPR8290(2)] (ab175186)
      Immunoprecipitation - Anti-xCT antibody [EPR8290(2)] (ab175186)

      ab175186 (purified) at 1:20 dilution (0.5µg) immunoprecipitating xCT in HepG2 whole cell lysate.
      Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab175186 & HepG2 whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab175186 in HepG2 whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Western blot - Anti-xCT antibody [EPR8290(2)] (ab175186)
      Western blot - Anti-xCT antibody [EPR8290(2)] (ab175186)
      All lanes : Anti-xCT antibody [EPR8290(2)] (ab175186) at 1/1000 dilution

      Lane 1 : A549 (Human lung carcinoma cell line) cell lysates
      Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysates
      Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysates
      Lane 4 : BxPC-3 (Human pancreas adenocarcinoma cell line) cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 55 kDa



      This image was generated using the unpurified version of the product. 

    • Immunoprecipitation - Anti-xCT antibody [EPR8290(2)] (ab175186)
      Immunoprecipitation - Anti-xCT antibody [EPR8290(2)] (ab175186)

      Western blot analysis on immunoprecipitation pellet from (Lane 1) HepG2 cells lysate or (Lane 2) 1 X PBS (negative control) using ab175186 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

      This image was generated using the unpurified version of the product. 

    References

    This product has been referenced in:

    • Wen X  et al. NOX1/NADPH oxidase regulates the expression of multidrug resistance-associated protein 1 and maintains intracellular glutathione levels. FEBS J 286:678-687 (2019). Read more (PubMed: 30653821) »
    • Lebourgeois S  et al. Evaluation of N-acetylcysteine on ethanol self-administration in ethanol-dependent rats. Neuropharmacology 150:112-120 (2019). Read more (PubMed: 30880122) »
    See all 9 Publications for this product

    Publishing research using ab175186? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-xCT antibody [EPR8290(2)]

     Good
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
    Permeabilization
    No
    Specification
    Spleen
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 07 2018

    Western blot abreview for Anti-xCT antibody [EPR8290(2)]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Brain tissue)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    8 µg
    Specification
    Brain tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 25 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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