Overview

  • Product name

  • Description

    Rabbit polyclonal to XIAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 282-470 of Human XIAP (Uniprot ID: P98170).

  • Positive control

    • A549 and NIH 3T3 whole cell lysates; HeLa cells; Human SW480 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab137392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 57 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0)

Target

  • Function

    Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B.
  • Tissue specificity

    Ubiquitous, except peripheral blood leukocytes.
  • Involvement in disease

    Defects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.
  • Sequence similarities

    Belongs to the IAP family.
    Contains 3 BIR repeats.
    Contains 1 RING-type zinc finger.
  • Domain

    The first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains.
  • Post-translational
    modifications

    Ubiquitinated and degraded by the proteasome in apoptotic cells.
    Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • AP 13 antibody
    • API3 antibody
    • Apoptosis Inhibitor 3 antibody
    • Baculoviral IAP repeat containing 4 antibody
    • Baculoviral IAP Repeat Containing Protein 4 antibody
    • Baculoviral IAP repeat-containing protein 4 antibody
    • BIRC 4 antibody
    • BIRC4 antibody
    • E3 ubiquitin-protein ligase XIAP antibody
    • hIAP-3 antibody
    • hIAP3 antibody
    • HILP antibody
    • IAP 3 antibody
    • IAP like protein antibody
    • IAP-3 antibody
    • IAP-like protein antibody
    • IAP3 antibody
    • ILP 1 antibody
    • ILP antibody
    • ILP1 antibody
    • Inhibitor of apoptosis protein 3 antibody
    • Inhibitor of Apoptosis X Linked antibody
    • Mammalian IAP Homologue A antibody
    • MIHA antibody
    • X linked IAP antibody
    • X linked inhibitor of apoptosis antibody
    • X linked inhibitor of apoptosis E3 ubiquitin protein ligase antibody
    • X linked inhibitor of apoptosis protein antibody
    • X-linked IAP antibody
    • X-linked inhibitor of apoptosis protein antibody
    • Xiap antibody
    • XIAP_HUMAN antibody
    • XLP2 antibody
    see all

Images

  • All lanes : Anti-XIAP antibody (ab137392) at 1/500 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : XIAP Knockout 293T cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 57 kDa
    Observed band size: 73 kDa
    why is the actual band size different from the predicted?

  • Anti-XIAP antibody (ab137392) at 1/1000 dilution + NIH 3T3 whole cell lysate
    at 30 µg

    Predicted band size: 57 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labelling XIAP with ab137392 at 1/200 dilution. Lower image is merged with DNA probe.
  • Immunohistochemical analysis of paraffin-embedded Human SW480 xenograft tissue, labelling XIAP with ab137392 at 1/500 dilution.
  • Anti-XIAP antibody (ab137392) at 1/1000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size: 57 kDa



    7.5% SDS PAGE

References

This product has been referenced in:

  • Márquez-Jurado S  et al. Mitochondrial levels determine variability in cell death by modulating apoptotic gene expression. Nat Commun 9:389 (2018). Read more (PubMed: 29374163) »
See 1 Publication for this product

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