Key features and details
- Rabbit polyclonal to XIAP
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-XIAP antibody
See all XIAP primary antibodies
DescriptionRabbit polyclonal to XIAP
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Rat
Recombinant full length protein (His-tag) corresponding to Human XIAP aa 1-497. Expressed in E.coli.
MTFNSFEGSKTCVPADINKEEEFVEEFNRLKTFANFPSGSPVSASTLARA GFLYTGEGDTVRCFSCHAAVDRWQYGDSAVGRHRKVSPNCRFINGFYLEN SATQSTNSGIQNGQYKVENYLGSRDHFALDRPSETHADYLLRTGQVVDIS DTIYPRNPAMYSEEARLKSFQNWPDYAHLTPRELASAGLYYTGIGDQVQC FCCGGKLKNWEPCDRAWSEHRRHFPNCFFVLGRNLNIRSESDAVSSDRNF PNSTNLPRNPSMADYEARIFTFGTWIYSVNKEQLARAGFYALGEGDKVKC FHCGGGLTDWKPSEDPWEQHAKWYPGCKYLLEQKGQEYINNIHLTHSLEE CLVRTTEKTPSLTRRIDDTIFQNPMVQEAIRMGFSFKDIKKIMEEKIQIS GSNYKSLEVLVADLVNAQKDSMQDESSQTSLQKEISTEEQLRRLQEEKLC KICMDRNIAIVFVPCGHLVTCKQCAEAVDKCPMCYTVITFKQKIFMS
Database link: P98170
- WB: Recombinant human XIAP; HepG2 and HEK-293T whole cell lysate; human heart and liver tissue lysate. IHC-P: Human kidney tissue. ICC/IF: HepG2 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 57 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|ICC/IF||Use a concentration of 5 - 20 µg/ml.|
FunctionApoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B.
Tissue specificityUbiquitous, except peripheral blood leukocytes.
Involvement in diseaseDefects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.
Sequence similaritiesBelongs to the IAP family.
Contains 3 BIR repeats.
Contains 1 RING-type zinc finger.
DomainThe first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains.
modificationsUbiquitinated and degraded by the proteasome in apoptotic cells.
Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation.
- Information by UniProt
- AP 13 antibody
- API3 antibody
- Apoptosis Inhibitor 3 antibody
Anti-XIAP antibody (ab233164) at 1 µg/ml + Recombinant human XIAP
HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 57 kDa
Formalin-fixed, paraffin-embedded human kidney tissue stained for XIAP using ab233164 at 20μg/ml in immunohistochemical analysis.
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for XIAP (green) using ab233164 at 20μg/ml in ICC/IF. FITC staining.
All lanes : Anti-XIAP antibody (ab233164) at 1 µg/ml
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Human heart tissue lysate
Lane 4 : Human liver tissue lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233164 has not yet been referenced specifically in any publications.