Recombinant
RabMAb

Recombinant Anti-XIAP antibody [EPR22189-113] - BSA and Azide free (ab238654)

Overview

  • Product name

    Anti-XIAP antibody [EPR22189-113] - BSA and Azide free
    See all XIAP primary antibodies
  • Description

    Rabbit monoclonal [EPR22189-113] to XIAP - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein within Mouse XIAP aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q60989

  • Positive control

    • WB: C6, RAW 264.7, PC-12, NIH/3T3 and Neuro-2a whole cell lysates.
  • General notes

    Ab238654 is the carrier-free version of ab229050. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238654 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab238654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 50-56 kDa (predicted molecular weight: 56 kDa).

Target

  • Function

    Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B.
  • Tissue specificity

    Ubiquitous, except peripheral blood leukocytes.
  • Involvement in disease

    Defects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.
  • Sequence similarities

    Belongs to the IAP family.
    Contains 3 BIR repeats.
    Contains 1 RING-type zinc finger.
  • Domain

    The first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains.
  • Post-translational
    modifications

    Ubiquitinated and degraded by the proteasome in apoptotic cells.
    Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • AP 13 antibody
    • API3 antibody
    • Apoptosis Inhibitor 3 antibody
    • Baculoviral IAP repeat containing 4 antibody
    • Baculoviral IAP Repeat Containing Protein 4 antibody
    • Baculoviral IAP repeat-containing protein 4 antibody
    • BIRC 4 antibody
    • BIRC4 antibody
    • E3 ubiquitin-protein ligase XIAP antibody
    • hIAP-3 antibody
    • hIAP3 antibody
    • HILP antibody
    • IAP 3 antibody
    • IAP like protein antibody
    • IAP-3 antibody
    • IAP-like protein antibody
    • IAP3 antibody
    • ILP 1 antibody
    • ILP antibody
    • ILP1 antibody
    • Inhibitor of apoptosis protein 3 antibody
    • Inhibitor of Apoptosis X Linked antibody
    • Mammalian IAP Homologue A antibody
    • MIHA antibody
    • X linked IAP antibody
    • X linked inhibitor of apoptosis antibody
    • X linked inhibitor of apoptosis E3 ubiquitin protein ligase antibody
    • X linked inhibitor of apoptosis protein antibody
    • X-linked IAP antibody
    • x-linked inhibitor of apopotosis proteins antibody
    • X-linked inhibitor of apoptosis protein antibody
    • Xiap antibody
    • XIAP_HUMAN antibody
    • XLP2 antibody
    see all

Images

  • All lanes : Anti-XIAP antibody [EPR22189-113] (ab229050) at 1/1000 dilution

    Lane 1 : C6 (rat glial tumor cell line) whole cell lysate
    Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 5 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 56 kDa
    Observed band size: 50-56 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229050).

References

ab238654 has not yet been referenced specifically in any publications.

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