Recombinant Anti-XIAP (phospho S87) antibody [EPR572(N)] (ab175935)
![Western blot - Anti-XIAP (phospho S87) antibody [EPR572(N)] (ab175935)](https://www.abcam.com/ps/products/175/ab175935/Images/ab175935-203041-anti-xiap-phospho-s87-antibody-epr572n-western-blot.jpg "Western blot - Anti-XIAP (phospho S87) antibody [EPR572(N)] (ab175935)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR572(N)] to XIAP (phospho S87)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-XIAP (phospho S87) antibody [EPR572(N)]
See all XIAP primary antibodies -
Description
Rabbit monoclonal [EPR572(N)] to XIAP (phospho S87) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human XIAP aa 50-150 (phospho S87) (Cysteine residue). The exact sequence is proprietary.
Database link: P98170 -
Positive control
- Okadaic acid and Calyculin A treated Hela cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR572(N) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175935 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 56 kDa.
|
| Notes |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 56 kDa. |
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
Target
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Function
Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. -
Tissue specificity
Ubiquitous, except peripheral blood leukocytes. -
Involvement in disease
Defects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma. -
Sequence similarities
Belongs to the IAP family.
Contains 3 BIR repeats.
Contains 1 RING-type zinc finger. -
Domain
The first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains. -
Post-translational
modificationsUbiquitinated and degraded by the proteasome in apoptotic cells.
Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 331 Human
- Omim: 300079 Human
- SwissProt: P98170 Human
- Unigene: 356076 Human
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Alternative names
- AP 13 antibody
- API3 antibody
- Apoptosis Inhibitor 3 antibody
see all
Images
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Western blot - Anti-XIAP (phospho S87) antibody [EPR572(N)] (ab175935)All lanes : Anti-XIAP (phospho S87) antibody [EPR572(N)] (ab175935) at 1/1000 dilution
Lane 1 : Hela cell lysate
Lane 2 : Hela cell lysate treated with Okadaic acid and Calyculin A
Lysates/proteins at 10 µg per lane.
Predicted band size: 56 kDa -
![Anti-XIAP (phospho S87) antibody [EPR572(N)] (ab175935)](https://www.abcam.com/ps/products/175/ab175935/Images/ab175935-2-benefits-of-recombinant-antibodies.png)
Anti-XIAP (phospho S87) antibody [EPR572(N)] (ab175935)
Datasheets and documents
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Datasheet download
References (2)
ab175935 has been referenced in 2 publications.
- Ali R et al. ATM Regulated PTEN Degradation Is XIAP E3 Ubiquitin Ligase Mediated in p85a Deficient Cancer Cells and Influence Platinum Sensitivity. Cells 8:N/A (2019). PubMed: 31635307
- Mejia C et al. Decreased activation of placental mTOR family members is associated with the induction of intrauterine growth restriction by secondhand smoke in the mouse. Cell Tissue Res N/A:N/A (2016). PubMed: 27613305
