Recombinant
Anti-XIAP (phospho S87) antibody [EPR572(N)] (ab175935)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-XIAP (phospho S87) antibody [EPR572(N)]
See all XIAP primary antibodies -
DescriptionRabbit monoclonal [EPR572(N)] to XIAP (phospho S87)
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivityReacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human XIAP aa 50-150 (phospho S87) (Cysteine residue). The exact sequence is proprietary.
Database link: P98170 -
Positive control
- Okadaic acid and Calyculin A treated Hela cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR572(N)
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab175935 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 56 kDa. |
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
Target
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FunctionApoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B.
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Tissue specificityUbiquitous, except peripheral blood leukocytes.
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Involvement in diseaseDefects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.
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Sequence similaritiesBelongs to the IAP family.
Contains 3 BIR repeats.
Contains 1 RING-type zinc finger. -
DomainThe first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains.
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Post-translational
modificationsUbiquitinated and degraded by the proteasome in apoptotic cells.
Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation. -
Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 331 Human
- Omim: 300079 Human
- SwissProt: P98170 Human
- Unigene: 356076 Human
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Alternative names
- AP 13 antibody
- API3 antibody
- Apoptosis Inhibitor 3 antibody
see all
Images
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![Western blot - Anti-XIAP (phospho S87) antibody [EPR572(N)] (ab175935)](https://www.abcam.com/ps/products/175/ab175935/Images/ab175935-203041-wb1.jpg)
Western blot - Anti-XIAP (phospho S87) antibody [EPR572(N)] (ab175935)All lanes : Anti-XIAP (phospho S87) antibody [EPR572(N)] (ab175935) at 1/1000 dilution
Lane 1 : Hela cell lysate
Lane 2 : Hela cell lysate treated with Okadaic acid and Calyculin A
Lysates/proteins at 10 µg per lane.
Predicted band size: 56 kDa
Datasheets and documents
Protocols
References
This product has been referenced in:
- Mejia C et al. Decreased activation of placental mTOR family members is associated with the induction of intrauterine growth restriction by secondhand smoke in the mouse. Cell Tissue Res N/A:N/A (2016). Read more (PubMed: 27613305) »
