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    xiap-phospho-s87-antibody-epr572n-ab175935.pdf

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Cell Biology Apoptosis Intracellular Survivin / IAPs
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RecombinantRabMAb

Recombinant Anti-XIAP (phospho S87) antibody [EPR572(N)] (ab175935)

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Western blot - Anti-XIAP (phospho S87) antibody [EPR572(N)] (ab175935)
  • Anti-XIAP (phospho S87) antibody [EPR572(N)] (ab175935)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR572(N)] to XIAP (phospho S87)
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-XIAP (phospho S87) antibody [EPR572(N)]
    See all XIAP primary antibodies
  • Description

    Rabbit monoclonal [EPR572(N)] to XIAP (phospho S87)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Okadaic acid and Calyculin A treated Hela cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR572(N)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Survivin / IAPs
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • Inhibitors
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Caspases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Death receptors & ligands
    • IAPs
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Caspases
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Death receptors & ligands
    • IAPs
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Alternative Versions

    • Anti-XIAP (phospho S87) antibody [EPR572(N)] - BSA and Azide free (ab249900)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human XIAP protein (ab95933)
  • Related Products

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab175935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 56 kDa.
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 56 kDa.
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

Target

  • Function

    Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B.
  • Tissue specificity

    Ubiquitous, except peripheral blood leukocytes.
  • Involvement in disease

    Defects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.
  • Sequence similarities

    Belongs to the IAP family.
    Contains 3 BIR repeats.
    Contains 1 RING-type zinc finger.
  • Domain

    The first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains.
  • Post-translational
    modifications

    Ubiquitinated and degraded by the proteasome in apoptotic cells.
    Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P98170 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 331 Human
    • Omim: 300079 Human
    • SwissProt: P98170 Human
    • Unigene: 356076 Human
    • Alternative names

      • AP 13 antibody
      • API3 antibody
      • Apoptosis Inhibitor 3 antibody
      • Baculoviral IAP repeat containing 4 antibody
      • Baculoviral IAP Repeat Containing Protein 4 antibody
      • Baculoviral IAP repeat-containing protein 4 antibody
      • BIRC 4 antibody
      • BIRC4 antibody
      • E3 ubiquitin-protein ligase XIAP antibody
      • hIAP-3 antibody
      • hIAP3 antibody
      • HILP antibody
      • IAP 3 antibody
      • IAP like protein antibody
      • IAP-3 antibody
      • IAP-like protein antibody
      • IAP3 antibody
      • ILP 1 antibody
      • ILP antibody
      • ILP1 antibody
      • Inhibitor of apoptosis protein 3 antibody
      • Inhibitor of Apoptosis X Linked antibody
      • Mammalian IAP Homologue A antibody
      • MIHA antibody
      • X linked IAP antibody
      • X linked inhibitor of apoptosis antibody
      • X linked inhibitor of apoptosis E3 ubiquitin protein ligase antibody
      • X linked inhibitor of apoptosis protein antibody
      • X-linked IAP antibody
      • x-linked inhibitor of apopotosis proteins antibody
      • X-linked inhibitor of apoptosis protein antibody
      • Xiap antibody
      • XIAP_HUMAN antibody
      • XLP2 antibody
      see all

    Images

    • Western blot - Anti-XIAP (phospho S87) antibody [EPR572(N)] (ab175935)
      Western blot - Anti-XIAP (phospho S87) antibody [EPR572(N)] (ab175935)
      All lanes : Anti-XIAP (phospho S87) antibody [EPR572(N)] (ab175935) at 1/1000 dilution

      Lane 1 : Hela cell lysate
      Lane 2 : Hela cell lysate treated with Okadaic acid and Calyculin A

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 56 kDa

    • Anti-XIAP (phospho S87) antibody [EPR572(N)] (ab175935)
      Anti-XIAP (phospho S87) antibody [EPR572(N)] (ab175935)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (2)

    Publishing research using ab175935? Please let us know so that we can cite the reference in this datasheet.

    ab175935 has been referenced in 2 publications.

    • Ali R  et al. ATM Regulated PTEN Degradation Is XIAP E3 Ubiquitin Ligase Mediated in p85a Deficient Cancer Cells and Influence Platinum Sensitivity. Cells 8:N/A (2019). PubMed: 31635307
    • Mejia C  et al. Decreased activation of placental mTOR family members is associated with the induction of intrauterine growth restriction by secondhand smoke in the mouse. Cell Tissue Res N/A:N/A (2016). PubMed: 27613305

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