Recombinant
RabMAb

Recombinant Anti-XIAP (phospho S87) antibody [EPR572(N)] (ab175935)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR572(N)] to XIAP (phospho S87)
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-XIAP (phospho S87) antibody [EPR572(N)]
    See all XIAP primary antibodies

  • Description

    Rabbit monoclonal [EPR572(N)] to XIAP (phospho S87)

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human XIAP aa 50-150 (phospho S87) (Cysteine residue). The exact sequence is proprietary.
    Database link: P98170

  • Positive control

    • Okadaic acid and Calyculin A treated Hela cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab175935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 56 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

    • Target

    • Function

      Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B.

    • Tissue specificity

      Ubiquitous, except peripheral blood leukocytes.

    • Involvement in disease

      Defects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.

    • Sequence similarities

      Belongs to the IAP family.
      Contains 3 BIR repeats.
      Contains 1 RING-type zinc finger.

    • Domain

      The first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains.

    • Post-translational
      modifications

      Ubiquitinated and degraded by the proteasome in apoptotic cells.
      Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      • Alternative names

        • AP 13 antibody
        • API3 antibody
        • Apoptosis Inhibitor 3 antibody
        • Baculoviral IAP repeat containing 4 antibody
        • Baculoviral IAP Repeat Containing Protein 4 antibody
        • Baculoviral IAP repeat-containing protein 4 antibody
        • BIRC 4 antibody
        • BIRC4 antibody
        • E3 ubiquitin-protein ligase XIAP antibody
        • hIAP-3 antibody
        • hIAP3 antibody
        • HILP antibody
        • IAP 3 antibody
        • IAP like protein antibody
        • IAP-3 antibody
        • IAP-like protein antibody
        • IAP3 antibody
        • ILP 1 antibody
        • ILP antibody
        • ILP1 antibody
        • Inhibitor of apoptosis protein 3 antibody
        • Inhibitor of Apoptosis X Linked antibody
        • Mammalian IAP Homologue A antibody
        • MIHA antibody
        • X linked IAP antibody
        • X linked inhibitor of apoptosis antibody
        • X linked inhibitor of apoptosis E3 ubiquitin protein ligase antibody
        • X linked inhibitor of apoptosis protein antibody
        • X-linked IAP antibody
        • x-linked inhibitor of apopotosis proteins antibody
        • X-linked inhibitor of apoptosis protein antibody
        • Xiap antibody
        • XIAP_HUMAN antibody
        • XLP2 antibody
        see all

      Images

      • Western blot - Anti-XIAP (phospho S87) antibody [EPR572(N)] (ab175935)
        Western blot - Anti-XIAP (phospho S87) antibody [EPR572(N)] (ab175935)
        All lanes : Anti-XIAP (phospho S87) antibody [EPR572(N)] (ab175935) at 1/1000 dilution

        Lane 1 : Hela cell lysate
        Lane 2 : Hela cell lysate treated with Okadaic acid and Calyculin A

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 56 kDa

      References (2)

      Publishing research using ab175935? Please let us know so that we can cite the reference in this datasheet.

      ab175935 has been referenced in 2 publications.

      • Ali R  et al. ATM Regulated PTEN Degradation Is XIAP E3 Ubiquitin Ligase Mediated in p85a Deficient Cancer Cells and Influence Platinum Sensitivity. Cells 8:N/A (2019). PubMed: 31635307
      • Mejia C  et al. Decreased activation of placental mTOR family members is associated with the induction of intrauterine growth restriction by secondhand smoke in the mouse. Cell Tissue Res N/A:N/A (2016). PubMed: 27613305

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