Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15882-36] to XLF - C-terminal
- Suitable for: Flow Cyt, ICC/IF, WB, IHC-P
- Knockout validated
- Reacts with: Human
Product nameAnti-XLF antibody [EPR15882-36] - C-terminal
See all XLF primary antibodies
DescriptionRabbit monoclonal [EPR15882-36] to XLF - C-terminal
Tested applicationsSuitable for: Flow Cyt, ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Recombinant fragment within Human XLF aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: Q9H9Q4
- Ramos, Jurkat and HepG2 whole cell lysate (ab7900); Human endometrial adenocarcinoma; HepG2 and NCCIT cells; Ramos cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab189917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/250 - 1/500.|
|WB||1/10000 - 1/50000. Detects a band of approximately 39 kDa (predicted molecular weight: 33 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionDNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary.
Tissue specificityUbiquitously expressed.
Involvement in diseaseDefects in NHEJ1 are the cause of severe combined immunodeficiency due to NHEJ1 deficiency (NHEJ1-SCID) [MIM:611291]; also known as autosomal recessive T cell-negative, B cell-negative, NK cell-positive, severe combined immunodeficiency with microcephaly, growth retardation and sensitivity to ionizing radiation or NHEJ1 syndrome. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. NHEJ1-SCID is characterized by a profound T- and B-lymphocytopenia associated with increased cellular sensitivity to ionizing radiation, microcephaly and growth retardation. Some patients may manifest SCID with sensitivity to ionizing radiation without microcephaly and mild growth retardation, probably due to hypomorphic NHEJ1 mutations.
Note=A chromosomal aberration involving NHEJ1 is found in a patient with polymicrogyria. Translocation t(2;7)(q35;p22).
Sequence similaritiesBelongs to the XLF family.
- Information by UniProt
- Cernunnos antibody
- FLJ12610 antibody
- NHEJ 1 antibody
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: XLF knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab189917 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab189917 was shown to specifically react with XLF in wild type cells as signal was lost in XLF knockout cells. Wild-type and XLF knockout samples were subjected to SDS-PAGE. ab189917 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Flow Cytometrical analysis of Ramos cells labeling XLF with ab189917 at 1/60 compared to a negative control cell. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunofluorescent analysis of paraformaldehyde-fixed NCCIT cells labeling XLF with ab189917 at 1/250, Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and DAPI staining (blue).
All lanes : Anti-XLF antibody [EPR15882-36] - C-terminal (ab189917) at 1/10000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDa
Additional bands at: 39 kDa. We are unsure as to the identity of these extra bands.
Immunofluorescent analysis of paraformaldehyde-fixed HepG2 cells labeling XLF with ab189917 at 1/250, Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and DAPI staining (blue).
Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling XLF with ab189917 at 1/250 with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ab189917 has been referenced in 1 publication.
- Li Z et al. Impaired DNA double-strand break repair contributes to the age-associated rise of genomic instability in humans. Cell Death Differ 23:1765-1777 (2016). PubMed: 27391797