Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15882-42] to XLF
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
Product nameAnti-XLF antibody [EPR15882-42]
See all XLF primary antibodies
DescriptionRabbit monoclonal [EPR15882-42] to XLF
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment within Human XLF aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: Q9H9Q4
- NCCIT, 293, HepG2 and Jurkat cell lysates; A431 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab189520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 37 kDa (predicted molecular weight: 33 kDa).|
|ICC/IF||1/250 - 1/500.|
FunctionDNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary.
Tissue specificityUbiquitously expressed.
Involvement in diseaseDefects in NHEJ1 are the cause of severe combined immunodeficiency due to NHEJ1 deficiency (NHEJ1-SCID) [MIM:611291]; also known as autosomal recessive T cell-negative, B cell-negative, NK cell-positive, severe combined immunodeficiency with microcephaly, growth retardation and sensitivity to ionizing radiation or NHEJ1 syndrome. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. NHEJ1-SCID is characterized by a profound T- and B-lymphocytopenia associated with increased cellular sensitivity to ionizing radiation, microcephaly and growth retardation. Some patients may manifest SCID with sensitivity to ionizing radiation without microcephaly and mild growth retardation, probably due to hypomorphic NHEJ1 mutations.
Note=A chromosomal aberration involving NHEJ1 is found in a patient with polymicrogyria. Translocation t(2;7)(q35;p22).
Sequence similaritiesBelongs to the XLF family.
- Information by UniProt
- Cernunnos antibody
- FLJ12610 antibody
- NHEJ 1 antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: XLF knockout HAP1 cell lysate (20 µg)
Lanes 1 - 2: Merged signal (red and green). Green – ab189520 observed at 38 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab189520 was shown to specifically react with XLF when XLF knockout samples were used. Wild-type and XLF knockout samples were subjected to SDS-PAGE. ab189520 and ab18058 (loading control to Vinculin) were both diluted at 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling XLF with ab189520 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with DAPI.
All lanes : Anti-XLF antibody [EPR15882-42] (ab189520) at 1/20000 dilution
Lane 1 : NCCIT cell lysate
Lane 2 : 293 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Predicted band size: 33 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
ab189520 has been referenced in 1 publication.
- Sikorski K et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371