Overview

  • Product name
    Anti-XPA antibody [5A2]
    See all XPA primary antibodies
  • Description
    Mouse monoclonal [5A2] to XPA
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    His-tagged recombinant human XPA (Xeroderma Pigmentosum group A) protein

  • General notes
    Works on pure and partially purified protein but not on crude cell extract.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    5A2
  • Myeloma
    Sp2
  • Isotype
    IgG1
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab44 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 31 kDa.

Target

  • Function
    Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation.
  • Tissue specificity
    Expressed in various cell lines and in skin fibroblasts.
  • Involvement in disease
    Defects in XPA are a cause of xeroderma pigmentosum complementation group A (XP-A) [MIM:278700]; also known as xeroderma pigmentosum type 1 (XP1). XP-A is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Group A patients show the most severe skin symptoms and progressive neurological disorders.
  • Sequence similarities
    Belongs to the XPA family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated by HERC2 leading to degradation by the proteasome.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA repair protein complementing XP A cells antibody
    • DNA repair protein complementing XP-A cells antibody
    • DNA repair protein complementing XPA cells antibody
    • Excision repair controlling antibody
    • Xeroderma pigmentosum 1 antibody
    • Xeroderma pigmentosum complementation group A antibody
    • Xeroderma pigmentosum group A complementing protein antibody
    • Xeroderma pigmentosum group A-complementing protein antibody
    • XP 1 antibody
    • XP1 antibody
    • xpa antibody
    • XPA_HUMAN antibody
    • Xpac antibody
    see all

References

This product has been referenced in:
  • Wei Q  et al. Expression of nucleotide excision repair proteins in lymphocytes as a marker of susceptibility to squamous cell carcinomas of the head and neck. Cancer Epidemiol Biomarkers Prev 14:1961-6 (2005). Read more (PubMed: 16103444) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for your enquiry regarding ab44. We have not mapped the epitope recognized by this antibody therefore we do not know if it will or will not recognized your truncated protein. All our other antibodies against XPA are likely to recognize the non truncated form (e.g. polyclonal antibodies) as well as the truncated form or we do not know where the epitope is. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

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Answer

Thank you for your enquiry. All the information that we have regarding this antibody is located on the online datasheet, and at this time, we are unaware of any publications/references that feature the use of ab44. If you have any more questions, please contact us again.

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Answer

Thank you for getting back to us and sharing your recent results. We are very sorry to hear that you are still having problem with this antibody. The protocol we have sent it to you is a general ELISA method. Looking at our order record, we can inform you that we have sold several vials of this product without any problem. We get this product from an academic source and originally it has not been tested for ELISA. However, one of our customers reported in 2001 that it worked nicely on ELISA application. We published his comment on-line, please take a look at John Butler's review on the top panel of the datasheet. We would advise perhaps to contact this researcher (you can send an-email to him directly by clicking on his name) to get some more details of his protocol and discuss this issue further. Certainly, we can offer you a replacement vial - free of charge, it is possible that the vial you received may have gone off during shipping and/or storage. If you would like to get it, please do let us know. We look forward to hearing from you soon.

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Answer

Thank you for your enquiry. We are very sorry to hear that you are having problem with this product. I have searched our database and found that this is a popular selling product and your feedback is the first we have received about it not working. Therefore, at this stage, I would suggest that there is either a problem with the vial you received, or modifications to your protocol are needed to obtain a positive result. I would like to make the following suggestions: It is very important to emphasize that when you coat the wells with the antigen, always use coating buffer instead of water. For ELISA, we would suggest using 1% (v/w) BSA and the blocking should be at least for 60 min. 10 min is too short!!! I will send you detailed protocol for you in my next e-mail.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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