Key features and details
- Rabbit polyclonal to XPA
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-XPA antibody
See all XPA primary antibodies
DescriptionRabbit polyclonal to XPA
Specificityab58464 detects endogenous levels of total XPA protein.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide derived from internal sequence of human XPA.
- Extracts from COLO205 cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab58464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 31 kDa).|
FunctionInvolved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation.
Tissue specificityExpressed in various cell lines and in skin fibroblasts.
Involvement in diseaseDefects in XPA are a cause of xeroderma pigmentosum complementation group A (XP-A) [MIM:278700]; also known as xeroderma pigmentosum type 1 (XP1). XP-A is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Group A patients show the most severe skin symptoms and progressive neurological disorders.
Sequence similaritiesBelongs to the XPA family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated by HERC2 leading to degradation by the proteasome.
- Information by UniProt
- DNA repair protein complementing XP A cells antibody
- DNA repair protein complementing XP-A cells antibody
- DNA repair protein complementing XPA cells antibody
All lanes : Anti-XPA antibody (ab58464) at 1/500 dilution
Lane 1 : Extracts from COLO205 cells
Lane 2 : Extracts from COLO205 cells with immunizing peptide
Predicted band size: 31 kDa
Observed band size: 41 kDa why is the actual band size different from the predicted?
ab58464 has not yet been referenced specifically in any publications.