Anti-XPA antibody (ab58464)
Key features and details
- Rabbit polyclonal to XPA
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-XPA antibody
See all XPA primary antibodies -
Description
Rabbit polyclonal to XPA -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide derived from internal sequence of human XPA.
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Positive control
- Extracts from COLO205 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab58464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 31 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 31 kDa). |
Target
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Function
Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. -
Tissue specificity
Expressed in various cell lines and in skin fibroblasts. -
Involvement in disease
Defects in XPA are a cause of xeroderma pigmentosum complementation group A (XP-A) [MIM:278700]; also known as xeroderma pigmentosum type 1 (XP1). XP-A is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Group A patients show the most severe skin symptoms and progressive neurological disorders. -
Sequence similarities
Belongs to the XPA family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated by HERC2 leading to degradation by the proteasome. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7507 Human
- Entrez Gene: 22590 Mouse
- Omim: 611153 Human
- SwissProt: P23025 Human
- SwissProt: Q64267 Mouse
- Unigene: 654364 Human
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Alternative names
- DNA repair protein complementing XP A cells antibody
- DNA repair protein complementing XP-A cells antibody
- DNA repair protein complementing XPA cells antibody
see all
Images
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All lanes : Anti-XPA antibody (ab58464) at 1/500 dilution
Lane 1 : Extracts from COLO205 cells
Lane 2 : Extracts from COLO205 cells with immunizing peptide
Predicted band size: 31 kDa
Observed band size: 41 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab58464 has been referenced in 1 publication.
- Schubert N et al. Genome Replication Is Associated With Release of Immunogenic DNA Waste. Front Immunol 13:880413 (2022). PubMed: 35634291