Anti-XPB antibody (ab245538)
Key features and details
- Rabbit polyclonal to XPB
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-XPB antibody
See all XPB primary antibodies -
Description
Rabbit polyclonal to XPB -
Host species
Rabbit -
Tested Applications & Species
Application Species IP HumanWB MouseHuman -
Immunogen
Synthetic peptide within Human XPB aa 732-782. The exact sequence is proprietary. NP_000113.1
Database link: P19447 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab245538 was affinity purified using an epitope specific to XPB immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab245538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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IP |
Human
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WB |
Mouse
Human
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All applications |
Rat
Cow
Orangutan
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Application | Abreviews | Notes |
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IP |
Use at 2-5 µg/mg of lysate.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 89 kDa.
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Notes |
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IP
Use at 2-5 µg/mg of lysate. |
WB
1/2000 - 1/10000. Predicted molecular weight: 89 kDa. |
Target
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Function
ATP-dependent 3'-5' DNA helicase, component of the core-TFIIH basal transcription factor, involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. Acts by opening DNA either around the RNA transcription start site or the DNA damage. -
Involvement in disease
Defects in ERCC3 are the cause of xeroderma pigmentosum complementation group B (XP-B) [MIM:610651]; also known as xeroderma pigmentosum II (XP2) or XP group B (XPB) or xeroderma pigmentosum group B combined with Cockayne syndrome (XP-B/CS). Xeroderma pigmentosum is an autosomal recessive pigmentary skin disorder characterized by solar hypersensitivity of the skin, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Some XP-B patients present features of Cockayne syndrome, including dwarfism, sensorineural deafness, microcephaly, mental retardation, pigmentary retinopathy, ataxia, decreased nerve conduction velocities.
Defects in ERCC3 are a cause of trichothiodystrophy photosensitive (TTDP) [MIM:601675]. TTDP is an autosomal recessive disease characterized by sulfur-deficient brittle hair and nails, ichthyosis, mental retardation, impaired sexual development, abnormal facies and cutaneous photosensitivity correlated with a nucleotide excision repair (NER) defect. Neonates with trichothiodystrophy and ichthyosis are usually born with a collodion membrane. The severity of the ichthyosis after the membrane is shed is variable, ranging from a mild to severe lamellar ichthyotic phenotype. There are no reports of skin cancer associated with TTDP. -
Sequence similarities
Belongs to the helicase family. RAD25/XPB subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 507984 Cow
- Entrez Gene: 2071 Human
- Entrez Gene: 13872 Mouse
- Entrez Gene: 100172725 Orangutan
- Entrez Gene: 291703 Rat
- Omim: 133510 Human
- SwissProt: Q1RMT1 Cow
- SwissProt: P19447 Human
see all -
Alternative names
- Basic transcription factor 2 89 kDa subunit antibody
- BTF 2 antibody
- BTF2 antibody
see all
Images
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All lanes : Anti-XPB antibody (ab245538) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 89 kDa
Exposure time: 3 minutesLysates prepared in NETN buffer.
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XPB was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245538 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245538 at 1 µg/ml.
Lane 1: ab245538 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with exposure time of 3 minutes.
Lysates prepared in NETN buffer.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab245538 has not yet been referenced specifically in any publications.