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    xpb-antibody-ab245538.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Nucl. Excision Repair
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Anti-XPB antibody (ab245538)

  • Datasheet
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Western blot - Anti-XPB antibody (ab245538)
  • Immunoprecipitation - Anti-XPB antibody (ab245538)

Key features and details

  • Rabbit polyclonal to XPB
  • Suitable for: IP, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-XPB antibody
    See all XPB primary antibodies
  • Description

    Rabbit polyclonal to XPB
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human XPB aa 732-782. The exact sequence is proprietary. NP_000113.1
    Database link: P19447

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245538 was affinity purified using an epitope specific to XPB immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Nucl. Excision Repair
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism
    • Purine and pyrimidine synthesis
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • NIH/3T3 whole cell lysate (ab7179)
    • Jurkat whole cell lysate (ab7899)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab245538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IP
Human
WB
Mouse
Human
All applications
Rat
Cow
Orangutan
Application Abreviews Notes
IP
Use at 2-5 µg/mg of lysate.
WB
1/2000 - 1/10000. Predicted molecular weight: 89 kDa.
Notes
IP
Use at 2-5 µg/mg of lysate.
WB
1/2000 - 1/10000. Predicted molecular weight: 89 kDa.

Target

  • Function

    ATP-dependent 3'-5' DNA helicase, component of the core-TFIIH basal transcription factor, involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. Acts by opening DNA either around the RNA transcription start site or the DNA damage.
  • Involvement in disease

    Defects in ERCC3 are the cause of xeroderma pigmentosum complementation group B (XP-B) [MIM:610651]; also known as xeroderma pigmentosum II (XP2) or XP group B (XPB) or xeroderma pigmentosum group B combined with Cockayne syndrome (XP-B/CS). Xeroderma pigmentosum is an autosomal recessive pigmentary skin disorder characterized by solar hypersensitivity of the skin, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Some XP-B patients present features of Cockayne syndrome, including dwarfism, sensorineural deafness, microcephaly, mental retardation, pigmentary retinopathy, ataxia, decreased nerve conduction velocities.
    Defects in ERCC3 are a cause of trichothiodystrophy photosensitive (TTDP) [MIM:601675]. TTDP is an autosomal recessive disease characterized by sulfur-deficient brittle hair and nails, ichthyosis, mental retardation, impaired sexual development, abnormal facies and cutaneous photosensitivity correlated with a nucleotide excision repair (NER) defect. Neonates with trichothiodystrophy and ichthyosis are usually born with a collodion membrane. The severity of the ichthyosis after the membrane is shed is variable, ranging from a mild to severe lamellar ichthyotic phenotype. There are no reports of skin cancer associated with TTDP.
  • Sequence similarities

    Belongs to the helicase family. RAD25/XPB subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P19447 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 507984 Cow
    • Entrez Gene: 2071 Human
    • Entrez Gene: 13872 Mouse
    • Entrez Gene: 100172725 Orangutan
    • Entrez Gene: 291703 Rat
    • Omim: 133510 Human
    • SwissProt: Q1RMT1 Cow
    • SwissProt: P19447 Human
    • SwissProt: P49135 Mouse
    • SwissProt: Q5RA62 Orangutan
    • SwissProt: Q4G005 Rat
    • Unigene: 469872 Human
    • Unigene: 282335 Mouse
    • Unigene: 44012 Rat
    see all
  • Alternative names

    • Basic transcription factor 2 89 kDa subunit antibody
    • BTF 2 antibody
    • BTF2 antibody
    • BTF2 p89 antibody
    • DNA excision repair protein ERCC-3 antibody
    • DNA repair protein complementing XP-B cells antibody
    • ERCC 3 antibody
    • ercc3 antibody
    • ERCC3_HUMAN antibody
    • Excision Repair Cross-complementing Rodent Repair deficiency complementation Group 3 antibody
    • GTF2H antibody
    • RAD 25 antibody
    • RAD25 antibody
    • TFIIH 89 kDa subunit antibody
    • TFIIH antibody
    • TFIIH basal transcription factor complex 89 kDa subunit antibody
    • TFIIH basal transcription factor complex helicase XPB subunit antibody
    • TFIIH p89 antibody
    • Xeroderma pigmentosum group B-complementing protein antibody
    see all

Images

  • Western blot - Anti-XPB antibody (ab245538)
    Western blot - Anti-XPB antibody (ab245538)
    All lanes : Anti-XPB antibody (ab245538) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 89 kDa


    Exposure time: 3 minutes


    Lysates prepared in NETN buffer.

  • Immunoprecipitation - Anti-XPB antibody (ab245538)
    Immunoprecipitation - Anti-XPB antibody (ab245538)

    XPB was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245538 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245538 at 1 µg/ml.

    Lane 1: ab245538 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 3 minutes.

    Lysates prepared in NETN buffer.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab245538? Please let us know so that we can cite the reference in this datasheet.

    ab245538 has not yet been referenced specifically in any publications.

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