Overview

  • Product name

    Anti-XPB antibody [EPR14161] - BSA and Azide free
    See all XPB primary antibodies
  • Description

    Rabbit monoclonal [EPR14161] to XPB - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Cynomolgus monkey, Orangutan
  • Immunogen

    Synthetic peptide within Human XPB aa 200-300. The exact sequence is proprietary.
    Database link: P19447

  • Positive control

    • ICC/IF: A549 cells.
  • General notes

    Ab236128 is the carrier-free version of ab190698. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab236128 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab236128 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Target

  • Function

    ATP-dependent 3'-5' DNA helicase, component of the core-TFIIH basal transcription factor, involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. Acts by opening DNA either around the RNA transcription start site or the DNA damage.
  • Involvement in disease

    Defects in ERCC3 are the cause of xeroderma pigmentosum complementation group B (XP-B) [MIM:610651]; also known as xeroderma pigmentosum II (XP2) or XP group B (XPB) or xeroderma pigmentosum group B combined with Cockayne syndrome (XP-B/CS). Xeroderma pigmentosum is an autosomal recessive pigmentary skin disorder characterized by solar hypersensitivity of the skin, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Some XP-B patients present features of Cockayne syndrome, including dwarfism, sensorineural deafness, microcephaly, mental retardation, pigmentary retinopathy, ataxia, decreased nerve conduction velocities.
    Defects in ERCC3 are a cause of trichothiodystrophy photosensitive (TTDP) [MIM:601675]. TTDP is an autosomal recessive disease characterized by sulfur-deficient brittle hair and nails, ichthyosis, mental retardation, impaired sexual development, abnormal facies and cutaneous photosensitivity correlated with a nucleotide excision repair (NER) defect. Neonates with trichothiodystrophy and ichthyosis are usually born with a collodion membrane. The severity of the ichthyosis after the membrane is shed is variable, ranging from a mild to severe lamellar ichthyotic phenotype. There are no reports of skin cancer associated with TTDP.
  • Sequence similarities

    Belongs to the helicase family. RAD25/XPB subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Basic transcription factor 2 89 kDa subunit antibody
    • BTF 2 antibody
    • BTF2 antibody
    • BTF2 p89 antibody
    • DNA excision repair protein ERCC-3 antibody
    • DNA repair protein complementing XP-B cells antibody
    • ERCC 3 antibody
    • ercc3 antibody
    • ERCC3_HUMAN antibody
    • Excision Repair Cross-complementing Rodent Repair deficiency complementation Group 3 antibody
    • GTF2H antibody
    • RAD 25 antibody
    • RAD25 antibody
    • TFIIH 89 kDa subunit antibody
    • TFIIH antibody
    • TFIIH basal transcription factor complex 89 kDa subunit antibody
    • TFIIH basal transcription factor complex helicase XPB subunit antibody
    • TFIIH p89 antibody
    • Xeroderma pigmentosum group B-complementing protein antibody
    see all

Images

  • Immunocytochemical analysis of 4% Paraformaldehyde fixed A549 cells, labeling XPB using ab190698 at a 1/500 dilution and Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at a 1/200 dilutiion and counter-stained with Dapi.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190698).

References

ab236128 has not yet been referenced specifically in any publications.

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