Anti-XPC antibody (ab245385)
Key features and details
- Rabbit polyclonal to XPC
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-XPC antibody
See all XPC primary antibodies -
Description
Rabbit polyclonal to XPC -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Dog, Chimpanzee, Monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide within Human XPC aa 890-940. The exact sequence is proprietary. (NP_004619.2).
Database link: Q01831 -
Positive control
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
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General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab245385 was affinity purified using an epitope specific to XPC immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab245385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-5 µg/mg of lysate.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 106 kDa.
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Notes |
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IP
Use at 2-5 µg/mg of lysate. |
WB
1/2000 - 1/10000. Predicted molecular weight: 106 kDa. |
Target
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Function
Involved in global genome nucleotide excision repair (GG-NER) by acting as damage sensing and DNA-binding factor component of the XPC complex. Has only a low DNA repair activity by itself which is stimulated by RAD23B and RAD23A. Has a preference to bind DNA containing a short single-stranded segment but not to damaged oligonucleotides. This feature is proposed to be related to a dynamic sensor function: XPC can rapidly screen duplex DNA for non-hydrogen-bonded bases by forming a transient nucleoprotein intermediate complex which matures into a stable recognition complex through an intrinsic single-stranded DNA-binding activity.
The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair. In vitro, the XPC:RAD23B dimer is sufficient to initiate NER; it preferentially binds to cisplatin and UV-damaged double-stranded DNA and also binds to a variety of chemically and structurally diverse DNA adducts. XPC:RAD23B contacts DNA both 5' and 3' of a cisplatin lesion with a preference for the 5' side. XPC:RAD23B induces a bend in DNA upon binding. XPC:RAD23B stimulates the activity of DNA glycosylases TDG and SMUG1. -
Involvement in disease
Defects in XPC are a cause of xeroderma pigmentosum complementation group C (XP-C) [MIM:278720]; also known as xeroderma pigmentosum III (XP3). XP-C is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. -
Sequence similarities
Belongs to the XPC family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated upon UV irradiation; the ubiquitination requires the UV-DDB complex, appears to be reversible and does not serve as a signal for degradation. -
Cellular localization
Nucleus. Cytoplasm. Omnipresent in the nucleus and consistently associates with and dissociates from DNA in the absence of DNA damage. Continuously shuttles between the cytoplasm and the nucleus, which is impeded by the presence of NER lesions. - Information by UniProt
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Database links
- Entrez Gene: 460195 Chimpanzee
- Entrez Gene: 476521 Dog
- Entrez Gene: 101147904 Gorilla
- Entrez Gene: 7508 Human
- Entrez Gene: 100174420 Orangutan
- Omim: 613208 Human
- SwissProt: Q01831 Human
- Unigene: 475538 Human
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Alternative names
- DNA repair protein complementing XP C cells antibody
- DNA repair protein complementing XP-C cells antibody
- DNA repair protein complementing XPC cells antibody
see all
Images
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All lanes : Anti-XPC antibody (ab245385) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 106 kDa
Exposure time: 3 seconds -
XPC was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) with ab245385 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245385 at 0.1 µg/ml.
Lane 1: ab245385 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab245385 has not yet been referenced specifically in any publications.