Anti-XPD antibody (ab102682)
Key features and details
- Rabbit polyclonal to XPD
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-XPD antibody
See all XPD primary antibodies -
Description
Rabbit polyclonal to XPD -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human XPD aa 270-545.
Database link: P18074 -
Positive control
- WB: MR32, U-87MG whole cell lysate. IHC-P: ES2 xenograft, mouse esophagus. ICC/IF: A431 cells, Mock and treated HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab102682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 87 kDa.
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IHC-P |
1/100 - 1/1000.
Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH8.0). |
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 87 kDa. |
IHC-P
1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH8.0). |
ICC/IF
1/100 - 1/1000. |
Target
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Function
ATP-dependent 5'-3' DNA helicase, component of the core-TFIIH basal transcription factor. Involved in nucleotide excision repair (NER) of DNA by opening DNA around the damage, and in RNA transcription by RNA polymerase II by anchoring the CDK-activating kinase (CAK) complex, composed of CDK7, cyclin H and MAT1, to the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers. -
Involvement in disease
Defects in ERCC2 are the cause of xeroderma pigmentosum complementation group D (XP-D) [MIM:278730]; also known as XP group D (XPD). Xeroderma pigmentosum is an autosomal recessive pigmentary skin disorder characterized by solar hypersensitivity of the skin, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Some XP-D patients present features of Cockayne syndrome, including dwarfism, sensorineural deafness, microcephaly, mental retardation, pigmentary retinopathy, ataxia, decreased nerve conduction velocities.
Defects in ERCC2 are a cause of trichothiodystrophy photosensitive (TTDP) [MIM:601675]. TTDP is an autosomal recessive disease characterized by sulfur-deficient brittle hair and nails, ichthyosis, mental retardation, impaired sexual development, abnormal facies and cutaneous photosensitivity correlated with a nucleotide excision repair (NER) defect. Neonates with trichothiodystrophy and ichthyosis are usually born with a collodion membrane. The severity of the ichthyosis after the membrane is shed is variable, ranging from a mild to severe lamellar ichthyotic phenotype. There are no reports of skin cancer associated with TTDP.
Defects in ERCC2 are the cause of cerebro-oculo-facio-skeletal syndrome type 2 (COFS2) [MIM:610756]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur. -
Sequence similarities
Belongs to the helicase family. RAD3/XPD subfamily.
Contains 1 helicase ATP-binding domain. -
Post-translational
modificationsISGylated. -
Cellular localization
Nucleus. Cytoplasm > cytoskeleton > spindle. - Information by UniProt
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Database links
- Entrez Gene: 100125238 Cow
- Entrez Gene: 2068 Human
- Entrez Gene: 13871 Mouse
- Entrez Gene: 308415 Rat
- Omim: 278730 Human
- SwissProt: A6QLJ0 Cow
- SwissProt: P18074 Human
- SwissProt: O08811 Mouse
see all -
Alternative names
- TFIIH 80 kDa subunit antibody
- Basic transcription factor 2 80 kDa subunit antibody
- BTF2 p80 antibody
see all
Images
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Paraffin embedded mouse esophagus tissue stained for ERCC2 using ab102682 at 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-XPD antibody (ab102682) at 1/1000 dilution
Lane 1 : IMR32 whole cell lysate
Lane 2 : U-87MG whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 87 kDa
7.5% SDS-PAGE -
Mock and treated HeLa cells stained for XPD (green) using ab102682 at 1/500 dilution in ICC/IF.
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ab102682 at 1/500 dilution, staining XPD in a paraffin-embedded ES2 xenograft.
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A431 cells stained for ERCC2 (green) using ab102682 at 1/500 dilution in ICC/IF.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab102682 has been referenced in 2 publications.
- Wang Y et al. Cloning of the XPD gene and its function in malignant melanoma cells. Oncol Lett 20:1803-1809 (2020). PubMed: 32724423
- Kunze S et al. New mutation in the mouse Xpd/Ercc2 gene leads to recessive cataracts. PLoS One 10:e0125304 (2015). IHC . PubMed: 25951169