Product nameAnti-XPD antibody [EPR9675]
See all XPD primary antibodies
DescriptionRabbit monoclonal [EPR9675] to XPD
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
Synthetic peptide within Human XPD. The exact sequence is proprietary.
Database link: P18074
- K562, U87-MG, HeLa, A431 and MCF7 cell lysates. Paraffin-embedded Human colon tissue. HeLa cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab167418 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 87 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/250 - 1/500.|
FunctionATP-dependent 5'-3' DNA helicase, component of the core-TFIIH basal transcription factor. Involved in nucleotide excision repair (NER) of DNA by opening DNA around the damage, and in RNA transcription by RNA polymerase II by anchoring the CDK-activating kinase (CAK) complex, composed of CDK7, cyclin H and MAT1, to the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers.
Involvement in diseaseDefects in ERCC2 are the cause of xeroderma pigmentosum complementation group D (XP-D) [MIM:278730]; also known as XP group D (XPD). Xeroderma pigmentosum is an autosomal recessive pigmentary skin disorder characterized by solar hypersensitivity of the skin, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Some XP-D patients present features of Cockayne syndrome, including dwarfism, sensorineural deafness, microcephaly, mental retardation, pigmentary retinopathy, ataxia, decreased nerve conduction velocities.
Defects in ERCC2 are a cause of trichothiodystrophy photosensitive (TTDP) [MIM:601675]. TTDP is an autosomal recessive disease characterized by sulfur-deficient brittle hair and nails, ichthyosis, mental retardation, impaired sexual development, abnormal facies and cutaneous photosensitivity correlated with a nucleotide excision repair (NER) defect. Neonates with trichothiodystrophy and ichthyosis are usually born with a collodion membrane. The severity of the ichthyosis after the membrane is shed is variable, ranging from a mild to severe lamellar ichthyotic phenotype. There are no reports of skin cancer associated with TTDP.
Defects in ERCC2 are the cause of cerebro-oculo-facio-skeletal syndrome type 2 (COFS2) [MIM:610756]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur.
Sequence similaritiesBelongs to the helicase family. RAD3/XPD subfamily.
Contains 1 helicase ATP-binding domain.
Cellular localizationNucleus. Cytoplasm > cytoskeleton > spindle.
- Information by UniProt
- TFIIH 80 kDa subunit antibody
- Basic transcription factor 2 80 kDa subunit antibody
- BTF2 p80 antibody
All lanes : Anti-XPD antibody [EPR9675] (ab167418) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : U87-MG cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A431 cell lysate
Lane 5 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP antibody at 1/2000 dilution
Predicted band size: 87 kDa
ab167418 showing +ve staining in Human ovarian carcinoma.
ab167418 showing +ve staining in Human lung adenocarcinoma.
ab167418 showing +ve staining in Human endometrial carcinoma.
ab167418 showing +ve staining in Human cervical carcinoma.
ab167418 showing +ve staining in Human normal brain.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling XPD with ab167418 at 1/50 dilution.
Immunofluorescent analysis of HeLa cells labeling XPD with ab167418 at 1/250 dilution.
This product has been referenced in:
- Ding H et al. Silencing of Xeroderma Pigmentosum Group D Gene Promotes Hepatoma Cell Growth by Reducing P53 Expression. Med Sci Monit 24:8015-8021 (2018). Read more (PubMed: 30409962) »
- Zhu QN et al. LncRNA H19 is a major mediator of doxorubicin chemoresistance in breast cancer cells through a cullin4A-MDR1 pathway. Oncotarget 8:91990-92003 (2017). Read more (PubMed: 29190892) »