Product nameAnti-XPF antibody
See all XPF primary antibodies
DescriptionRabbit polyclonal to XPF
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
Recombinant fragment within Human XPF (internal sequence). The exact sequence is proprietary.
Database link: Q92889
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227712 in the following tested applications.
|WB||1/500 - 1/3000. Predicted molecular weight: 104 kDa.|
FunctionStructure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair. Involved in homologous recombination that assists in removing interstrand cross-link.
Involvement in diseaseDefects in ERCC4 are the cause of xeroderma pigmentosum complementation group F (XP-F) [MIM:278760]; also known as xeroderma pigmentosum VI (XP6). XP-F is an autosomal recessive disease characterized by hypersensitivity of the skin to sunlight followed by high incidence of skin cancer and frequent neurologic abnormalities.
Defects in ERCC4 are a cause of XFE progeroid syndrome (XFEPS) [MIM:610965]. This syndrome is illustrated by one patient who presented with dwarfism, cachexia and microcephaly.
Sequence similaritiesBelongs to the XPF family.
Contains 1 ERCC4 domain.
- Information by UniProt
- DNA excision repair protein ERCC 4 antibody
- DNA excision repair protein ERCC-4 antibody
- DNA excision repair protein ERCC4 antibody
All lanes : Anti-XPF antibody (ab227712) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 104 kDa
5% SDS-PAGE gel.
ab227712 has not yet been referenced specifically in any publications.