Overview

  • Product name

  • Description

    Rabbit polyclonal to XPF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from an internal sequence of human XPF.

  • Positive control

    • Extracts from 293 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab73720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 103 kDa.
ELISA 1/10000.
IP Use at an assay dependent concentration. PubMed: 24726326

Target

  • Function

    Structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair. Involved in homologous recombination that assists in removing interstrand cross-link.
  • Involvement in disease

    Defects in ERCC4 are the cause of xeroderma pigmentosum complementation group F (XP-F) [MIM:278760]; also known as xeroderma pigmentosum VI (XP6). XP-F is an autosomal recessive disease characterized by hypersensitivity of the skin to sunlight followed by high incidence of skin cancer and frequent neurologic abnormalities.
    Defects in ERCC4 are a cause of XFE progeroid syndrome (XFEPS) [MIM:610965]. This syndrome is illustrated by one patient who presented with dwarfism, cachexia and microcephaly.
  • Sequence similarities

    Belongs to the XPF family.
    Contains 1 ERCC4 domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • DNA excision repair protein ERCC 4 antibody
    • DNA excision repair protein ERCC-4 antibody
    • DNA excision repair protein ERCC4 antibody
    • DNA repair endonuclease XPF antibody
    • DNA repair protein complementing XP F cells antibody
    • DNA repair protein complementing XP-F cells antibody
    • ERCC 11 antibody
    • ERCC 4 antibody
    • ERCC11 antibody
    • ERCC4 antibody
    • Excision repair complementing defective in Chinese hamster antibody
    • Excision repair cross complementing rodent repair deficiency complementation group 4 antibody
    • excision repair cross-complementation group 4 antibody
    • FANCQ antibody
    • RAD 1 antibody
    • RAD1 antibody
    • Xeroderma pigmentosum complementation group F antibody
    • Xeroderma pigmentosum group F complementing protein antibody
    • Xeroderma pigmentosum group F-complementing protein antibody
    • Xeroderma pigmentosum VI antibody
    • XP, group G antibody
    • XP6 antibody
    • XPF_HUMAN antibody
    see all

Images

  • All lanes : Anti-XPF antibody (ab73720) at 1/500 dilution

    Lane 1 : Extracts from 293 cells
    Lane 2 : Extracts from 293 cells with immunizing peptide at 5 µg

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 103 kDa
    Observed band size: 103 kDa

References

This product has been referenced in:

  • Abdullah UB  et al. RPA activates the XPF-ERCC1 endonuclease to initiate processing of DNA interstrand crosslinks. EMBO J 36:2047-2060 (2017). WB . Read more (PubMed: 28607004) »
  • Hodskinson MR  et al. Mouse SLX4 is a tumor suppressor that stimulates the activity of the nuclease XPF-ERCC1 in DNA crosslink repair. Mol Cell 54:472-84 (2014). WB, IP ; Mouse . Read more (PubMed: 24726326) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (GM00637 fibroblast cell line)
Loading amount
100 µg
Specification
GM00637 fibroblast cell line
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
(agent) for 30 minute(s) · Concentration: 5µg/mL · Temperature: 20°C

Mrs. Annika Schäfer

Verified customer

Submitted Apr 16 2009

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