Key features and details
- Rabbit polyclonal to XPG
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-XPG antibody
See all XPG primary antibodies
DescriptionRabbit polyclonal to XPG
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Synthetic peptide derived from an internal sequence within Human XPG.
- Extracts from K562 cells
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab64931 was affinity-purified from rabbit antiserum by affinity-chromatography using an epitope-specific immunogen.
Our Abpromise guarantee covers the use of ab64931 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 133 kDa).|
FunctionSingle-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3'incision in DNA nucleotide excision repair (NER). Acts as a cofactor for a DNA glycosylase that removes oxidized pyrimidines from DNA. May also be involved in transcription-coupled repair of this kind of damage, in transcription by RNA polymerase II, and perhaps in other processes too.
Involvement in diseaseDefects in ERCC5 are the cause of xeroderma pigmentosum complementation group G (XP-G) [MIM:278780]; also known as xeroderma pigmentosum VII (XP7). Xeroderma pigmentosum is an autosomal recessive pigmentary skin disorder characterized by solar hypersensitivity of the skin, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Some XP-G patients present features of Cockayne syndrome, including dwarfism, sensorineural deafness, microcephaly, mental retardation, pigmentary retinopathy, ataxia, decreased nerve conduction velocities.
Sequence similaritiesBelongs to the XPG/RAD2 endonuclease family. XPG subfamily.
- Information by UniProt
- COFS 3 antibody
- COFS3 antibody
- DNA excision repair protein ERCC 5 antibody
All lanes : Anti-XPG antibody (ab64931) at 1/500 dilution
Lane 1 : Extracts from K562 cells
Lane 2 : Extracts from K562 cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 133 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
ab64931 has been referenced in 1 publication.
- Gaddameedhi S et al. Similar nucleotide excision repair capacity in melanocytes and melanoma cells. Cancer Res 70:4922-30 (2010). WB ; Human . PubMed: 20501836