Anti-XPNPEP1 antibody (ab235324)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-XPNPEP1 antibody
See all XPNPEP1 primary antibodies -
Description
Rabbit polyclonal to XPNPEP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Human XPNPEP1 aa 2-623.
Sequence:PPKVTSELLRQLRQAMRNSEYVTEPIQAYIIPSGDAHQSEYIAPCDCRRA FVSGFDGSAGTAIITEEHAAMWTDGRYFLQAAKQMDSNWTLMKMGLKDTP TQEDWLVSVLPEGSRVGVDPLIIPTDYWKKMAKVLRSAGHHLIPVKENLV DKIWTDRPERPCKPLLTLGLDYTGISWKDKVADLRLKMAERNVMWFVVTA LDEIAWLFNLRGSDVEHNPVFFSYAIIGLETIMLFIDGDRIDAPSVKEHL LLDLGLEAEYRIQVHPYKSILSELKALCADLSPREKVWVSDKASYAVSET IPKDHRCCMPYTPICIAKAVKNSAESEGMRRAHIKDAVALCELFNWLEKE VPKGGVTEISAADKAEEFRRQQADFVDLSFPTISSTGPNGAIIHYAPVPE TNRTLSLDEVYLIDSGAQYKDGTTDVTRTMHFGTPTAYEKECFTYVLKGH IAVSAAVFPTGTKGHLLDSFARSALWDSGLDYLHGTGHGVGSFLNVHEGP CGISYKTFSDEPLEAGMIVTDEPGYYEDGAFGIRIENVVLVVPVKTKYNF NNRGSLTFEPLTLVPIQTKMIDVDSLTDKECDWLNNYHLTCRDVIGKELQ KQGRQEALEWLIRETQPISKQH
Database link: Q9NQW7-1 -
Positive control
- WB: Mouse small intestine, stomach and kidney lysates. IHC-P: Human colon cancer tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab235324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 70 kDa. | |
| IHC-P | 1/20 - 1/200. |
Target
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Function
Contributes to the degradation of bradykinin. Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro. -
Tissue specificity
Expressed in all tissues tested, including pancreas, heart, muscle, kidney, liver, lung and brain. Highest levels in pancreas. -
Sequence similarities
Belongs to the peptidase M24B family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 7511 Human
- Entrez Gene: 170750 Mouse
- Omim: 602443 Human
- SwissProt: Q9NQW7 Human
- SwissProt: Q6P1B1 Mouse
- Unigene: 390623 Human
- Unigene: 99776 Mouse
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Form
X-prolyl aminopeptidase is a proline-specific metalloaminopeptidase that specifically catalyzes the removal of any unsubstituted N-terminal amino acid that is adjacent to a penultimate proline residue. There are 2 isoforms produced by alternative splicing. -
Alternative names
- Aminoacylproline aminopeptidase antibody
- aminopeptidase P, cytosolic antibody
- APP1 antibody
see all
Images
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Western blot - Anti-XPNPEP1 antibody (ab235324)All lanes : Anti-XPNPEP1 antibody (ab235324) at 1/1000 dilution
Lane 1 : Mouse small intestine lysate
Lane 2 : Mouse stomach lysate
Lane 3 : Mouse kidney lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 70 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPNPEP1 antibody (ab235324)Paraffin-embedded human colon cancer tissue stained for XPNPEP1 using ab235324 at 1/100 dilution in immunohistochemical analysis.
References
ab235324 has not yet been referenced specifically in any publications.
