Product nameAnti-XPNPEP1 antibody [OTI1E3]
See all XPNPEP1 primary antibodies
DescriptionMouse monoclonal [OTI1E3] to XPNPEP1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human XPNPEP1. Produced in HEK293T cells (NP_065116).
Database link: Q9NQW7
- WB: HEK-293T cells transfected with pCMV6-ENTRY XPNPEP1 cDNA; HepG2, HeLa, SVT2, A549, COS-7, Jurkat, MDCK, PC12 and MCF7 whole cell lysate. IHC-P: Human thyroid and prostate tissues. ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY XPNPEP1.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein G purified
Purification notesab123929 is purified from Mouse ascites fluid by affinity chromatography.
Our Abpromise guarantee covers the use of ab123929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 70 kDa.|
|IHC-P||1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionContributes to the degradation of bradykinin. Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro.
Tissue specificityExpressed in all tissues tested, including pancreas, heart, muscle, kidney, liver, lung and brain. Highest levels in pancreas.
Sequence similaritiesBelongs to the peptidase M24B family.
- Information by UniProt
FormX-prolyl aminopeptidase is a proline-specific metalloaminopeptidase that specifically catalyzes the removal of any unsubstituted N-terminal amino acid that is adjacent to a penultimate proline residue. There are 2 isoforms produced by alternative splicing.
- Aminoacylproline aminopeptidase antibody
- aminopeptidase P, cytosolic antibody
- APP1 antibody
COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY XPNPEP1, stained for XPNPEP1 (Green) using ab123929 at 1/100 dilution in ICC/IF.
Paraffin-embedded human thyroid tissue stained for XPNPEP1 using ab123929 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human prostate tissue stained for XPNPEP1 using ab123929 at 1/150 dilution in immunohistochemical analysis.
All lanes : Anti-XPNPEP1 antibody [OTI1E3] (ab123929) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY XPNPEP1 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 70 kDa
All lanes : Anti-XPNPEP1 antibody [OTI1E3] (ab123929) at 1/200 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : SVT2
Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 7 : MDCK
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 9 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 70 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab123929 has been referenced in 1 publication.
- Drendel V et al. Proteome profiling of clear cell renal cell carcinoma in von Hippel-Lindau patients highlights upregulation of Xaa-Pro aminopeptidase-1, an anti-proliferative and anti-migratory exoprotease. Oncotarget 8:100066-100078 (2017). PubMed: 29245961