Overview

  • Product name

    Anti-XPNPEP1 antibody [OTI1E3]
    See all XPNPEP1 primary antibodies
  • Description

    Mouse monoclonal [OTI1E3] to XPNPEP1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human XPNPEP1. Produced in HEK293T cells (NP_065116).
    Database link: Q9NQW7

  • Positive control

    • WB: HEK-293T cells transfected with pCMV6-ENTRY XPNPEP1 cDNA; HepG2, HeLa, SVT2, A549, COS-7, Jurkat, MDCK, PC12 and MCF7 whole cell lysate. IHC-P: Human thyroid and prostate tissues. ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY XPNPEP1.

Properties

Applications

Our Abpromise guarantee covers the use of ab123929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 70 kDa.
IHC-P 1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100.

Target

  • Function

    Contributes to the degradation of bradykinin. Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro.
  • Tissue specificity

    Expressed in all tissues tested, including pancreas, heart, muscle, kidney, liver, lung and brain. Highest levels in pancreas.
  • Sequence similarities

    Belongs to the peptidase M24B family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Form

    X-prolyl aminopeptidase is a proline-specific metalloaminopeptidase that specifically catalyzes the removal of any unsubstituted N-terminal amino acid that is adjacent to a penultimate proline residue. There are 2 isoforms produced by alternative splicing.
  • Alternative names

    • Aminoacylproline aminopeptidase antibody
    • aminopeptidase P, cytosolic antibody
    • APP1 antibody
    • Cytosolic aminopeptidase P antibody
    • RP11 451M19.1 antibody
    • sAmp antibody
    • Soluble aminopeptidase P antibody
    • soluble antibody
    • X Pro aminopeptidase 1 antibody
    • X prolyl aminopeptidase (aminopeptidase P) 1 antibody
    • X prolyl aminopeptidase (Aminopeptidase P) 1 soluble antibody
    • X prolyl aminopeptidase 1 antibody
    • X prolyl aminopeptidase 1 soluble antibody
    • X-Pro aminopeptidase 1 antibody
    • X-prolyl aminopeptidase 1 antibody
    • Xaa Pro aminopeptidase 1 antibody
    • Xaa-Pro aminopeptidase 1 antibody
    • XPNPEP 1 antibody
    • XPNPEP antibody
    • xpnpep1 antibody
    • XPNPEPL antibody
    • XPNPEPL1 antibody
    • XPP1_HUMAN antibody
    see all

Images

  • COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY XPNPEP1, stained for XPNPEP1 (Green) using ab123929 at 1/100 dilution in ICC/IF.

     

  • Paraffin-embedded human thyroid tissue stained for XPNPEP1 using ab123929 at 1/150 dilution in immunohistochemical analysis.

  • Paraffin-embedded human prostate tissue stained for XPNPEP1 using ab123929 at 1/150 dilution in immunohistochemical analysis.

  • All lanes : Anti-XPNPEP1 antibody [OTI1E3] (ab123929) at 1/2000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY XPNPEP1 cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 70 kDa

  • All lanes : Anti-XPNPEP1 antibody [OTI1E3] (ab123929) at 1/200 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : SVT2
    Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
    Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 7 : MDCK
    Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 9 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 70 kDa

References

This product has been referenced in:

  • Drendel V  et al. Proteome profiling of clear cell renal cell carcinoma in von Hippel-Lindau patients highlights upregulation of Xaa-Pro aminopeptidase-1, an anti-proliferative and anti-migratory exoprotease. Oncotarget 8:100066-100078 (2017). Read more (PubMed: 29245961) »
See 1 Publication for this product

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