Overview

  • Product name

    Anti-XPOT antibody
  • Description

    Rabbit polyclonal to XPOT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Orangutan
  • Immunogen

    Synthetic peptide within Human XPOT aa 912-962. The exact sequence is proprietary. (NP_009166.2).
    Database link: O43592

  • Positive control

    • WB: HEK-293T, HeLa and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.
  • General notes

     This product was previously labelled as Exportin T

     

Properties

Applications

Our Abpromise guarantee covers the use of ab241474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 110 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Mediates the nuclear export of aminoacylated tRNAs. In the nucleus binds to tRNA and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the tRNA from the export receptor. XPOT then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
  • Sequence similarities

    Belongs to the exportin family.
  • Cellular localization

    Nucleus. Cytoplasm. Nuclear, once bound to tRNA and Ran the complex translocates to the cytoplasm. Shuttles between the nucleus and the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1110004L07Rik antibody
    • 3110065H13Rik antibody
    • AI452076 antibody
    • C79645 antibody
    • Exportin tRNA antibody
    • Exportin(tRNA) antibody
    • Exportin-T antibody
    • Nuclear export receptor for tRNAs antibody
    • tRNA exportin antibody
    • XPO3 antibody
    • xpot antibody
    • XPOT_HUMAN antibody
    see all

Images

  • All lanes : Anti-XPOT antibody (ab241474) at 0.4 µg/ml

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 110 kDa


    Exposure time: 3 minutes
  • XPOT was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241474 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab241474 at 1 µg/ml.

    Lane 1: ab241474 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 3 minutes.

References

ab241474 has not yet been referenced specifically in any publications.

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