Overview

  • Product name
    Anti-XRCC1 antibody [33-2-5]
    See all XRCC1 primary antibodies
  • Description
    Mouse monoclonal [33-2-5] to XRCC1
  • Host species
    Mouse
  • Specificity
    This antibody is specific for XRCC1 (85 kDa).
  • Tested applications
    Suitable for: WB, IP, IHC-P, IHC-Fr, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human, Chinese hamster
  • Immunogen

    Recombinant full length protein corresponding to Human XRCC1.

  • Positive control
    • IHC-P: Human testis tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab1838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 85 kDa.
IP Use at an assay dependent concentration. PubMed: 17982170
IHC-P 1/50 - 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/50 - 1/100.
ICC/IF 1/200.
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
  • Sequence similarities
    Contains 2 BRCT domains.
  • Post-translational
    modifications
    Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
    Sumoylated.
  • Cellular localization
    Nucleus. Accumulates at sites of DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA repair protein XRCC1 antibody
    • RCC antibody
    • X ray repair complementing defective repair in chinese hamster antibody
    • X ray Repair Complementing Defective Repair in Chinese Hamster Cells antibody
    • X ray repair complementing defective repair in chinese hamster cells 1 antibody
    • X ray repair cross complementing 1 antibody
    • X ray repair cross complementing protein 1 antibody
    • X ray repair, complementing defective, repair in Chinese hamster antibody
    • X-ray repair cross-complementing protein 1 antibody
    • XRCC 1 antibody
    • Xrcc1 antibody
    • XRCC1_HUMAN antibody
    see all

Images

  • All lanes : Anti-XRCC1 antibody [33-2-5] (ab1838) at 1/500 dilution

    Lane 1 : Human dermal fibroblast - whole cell lysate. Treated with non-targeting siRNA
    Lane 2 : Human dermal fibroblast - whole cell lysate. Treated with XRCC1 siRNA

    Secondary
    All lanes : HRP-conjugated rabbit anti-mouse IgG at 1/2500 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 85 kDa
    Observed band size: 85 kDa


    Exposure time: 30 seconds

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling XRCC1 with ab1838.

  • ab1838 staining XRCC1 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 4% BSA for 10 minutes. Samples were incubated with primary antibody (1/200) for 1 hour. An undiluted Alexa Fluor® 555-conjugated donkey anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab1838 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1838, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Ronson GE  et al. PARP1 and PARP2 stabilise replication forks at base excision repair intermediates through Fbh1-dependent Rad51 regulation. Nat Commun 9:746 (2018). Read more (PubMed: 29467415) »
  • Galanos P  et al. Mutational signatures reveal the role of RAD52 in p53-independent p21-driven genomic instability. Genome Biol 19:37 (2018). Read more (PubMed: 29548335) »
See all 41 Publications for this product

Customer reviews and Q&As

1-10 of 13 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mef)
Loading amount
40 µg
Specification
mef
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Abcam user community

Verified customer

Submitted May 16 2012

Answer

Thank you for your reply.

I am very pleased to hear you would like to accept our offer and test ab1838 in mouse. As requested, I am providing the following testing discount code:

DISCOUNT CODE: #####
Expiration date: #####

This code will give you 1 free primary antibody] before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Question
Answer

Thank you for your telephone call earlier today.

As discussed on teh phone, as far as we are aware, ab1838 has never been tested with samples from mouse]. All tested species cross-reactivity information is stated on our datasheets, and these are updated as soon as any new information is brought to our attention.

I have checked the alignment of the immunogen (full length human protein) with the mouse protein, and this is fairly high at 86%. We would therefore suggest that this antibody should detect in mouse. However, I am sorry we are not able to guarantee this without further testing.

Therefore, I can offer a discount off a future purchase if you buy ab1838 now, test it in mouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab1838 in mouse. I will then send a discount code. This code must be issued before purchasing ab1838 so please wait for my reply before ordering.

2. Purchase ab1838 either by phone, fax, or online (www.abcam.com).

3. Test it in mouse.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab1838 turns out to be unsuitable for mouse, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (U2OS)
Loading amount
25 µg
Specification
U2OS
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Dr. Andrew Cobb

Verified customer

Submitted Mar 29 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
30 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (10% Tris-Gly gel)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Nov 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
5 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (acrylamide 9%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 25 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Dermal Fibroblast)
Loading amount
10000 cells
Specification
Dermal Fibroblast
Treatment
XRCC1 or non-targeting siRNA
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 17 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Dermal Fibroblast)
Specification
Dermal Fibroblast
Fixative
Formaldehyde
Permeabilization
Yes - Triton
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10%

Abcam user community

Verified customer

Submitted Aug 28 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (cervix (Hela))
Specification
cervix (Hela)
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%

Dr. Alexander Rapp

Verified customer

Submitted Feb 01 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela)
Specification
Hela
Fixative
Formaldehyde
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 4%

Mr. Oliver Mortusewicz

Verified customer

Submitted Oct 17 2006

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up