Overview

  • Product name

  • Description

    Chicken polyclonal to XRCC1
  • Host species

    Chicken
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chinese hamster
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WB: U-2 OS and Molt4 whole cell lysate. ICC/IF: U-2 OS cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab235196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 69 kDa.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
  • Sequence similarities

    Contains 2 BRCT domains.
  • Post-translational
    modifications

    Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
    Sumoylated.
  • Cellular localization

    Nucleus. Accumulates at sites of DNA damage.
  • Information by UniProt
  • Database links

  • Alternative names

    • DNA repair protein XRCC1 antibody
    • RCC antibody
    • X ray repair complementing defective repair in chinese hamster antibody
    • X ray Repair Complementing Defective Repair in Chinese Hamster Cells antibody
    • X ray repair complementing defective repair in chinese hamster cells 1 antibody
    • X ray repair cross complementing 1 antibody
    • X ray repair cross complementing protein 1 antibody
    • X ray repair, complementing defective, repair in Chinese hamster antibody
    • X-ray repair cross-complementing protein 1 antibody
    • XRCC 1 antibody
    • Xrcc1 antibody
    • XRCC1_HUMAN antibody
    see all

Images

  • All lanes : Anti-XRCC1 antibody (ab235196) at 5 µg/ml

    Lane 1 : U-2 OS whole cell lysate
    Lane 2 : Molt4 whole cell lysate
    Lane 3 : Human testis whole tissue lysate
    Lane 4 : Mouse liver whole tissue lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    All lanes : Goat anti-Chicken IgG [H&L] at 1/40000 dilution

    Predicted band size: 69 kDa


    Exposure time: 20 seconds
  • 100% methanol-fixed, 0.3% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling XRCC1 using ab235196 at 10 µg/ml, overnight at +4°C. Followed by a Donkey anti-Chicken IgG DyLight™ 488 conjugated preadsorbed secondary antibody at 5 µg/ml, for 1 hour at RT.

References

ab235196 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab235196.
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