Product nameAnti-XRCC1 antibody
See all XRCC1 primary antibodies
DescriptionChicken polyclonal to XRCC1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chinese hamster
Synthetic peptide. This information is considered to be commercially sensitive.
- WB: U-2 OS and Molt4 whole cell lysate. ICC/IF: U-2 OS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Concentration information loading...
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Purine and pyrimidine synthesis
Our Abpromise guarantee covers the use of ab235196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 69 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionCorrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
Sequence similaritiesContains 2 BRCT domains.
modificationsPhosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
Cellular localizationNucleus. Accumulates at sites of DNA damage.
- Information by UniProt
- DNA repair protein XRCC1 antibody
- RCC antibody
- X ray repair complementing defective repair in chinese hamster antibody
All lanes : Anti-XRCC1 antibody (ab235196) at 5 µg/ml
Lane 1 : U-2 OS whole cell lysate
Lane 2 : Molt4 whole cell lysate
Lane 3 : Human testis whole tissue lysate
Lane 4 : Mouse liver whole tissue lysate
Lysates/proteins at 35 µg per lane.
All lanes : Goat anti-Chicken IgG [H&L] at 1/40000 dilution
Predicted band size: 69 kDa
Exposure time: 20 seconds
100% methanol-fixed, 0.3% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling XRCC1 using ab235196 at 10 µg/ml, overnight at +4°C. Followed by a Donkey anti-Chicken IgG DyLight™ 488 conjugated preadsorbed secondary antibody at 5 µg/ml, for 1 hour at RT.
ab235196 has not yet been referenced specifically in any publications.