Key features and details
- Chicken polyclonal to XRCC1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgY
Product nameAnti-XRCC1 antibody
See all XRCC1 primary antibodies
DescriptionChicken polyclonal to XRCC1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chinese hamster
Synthetic peptide. This information is considered to be commercially sensitive.
- WB: U-2 OS and Molt4 whole cell lysate. ICC/IF: U-2 OS cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Concentration information loading...
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Purine and pyrimidine synthesis
Our Abpromise guarantee covers the use of ab235196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 69 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionCorrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
Sequence similaritiesContains 2 BRCT domains.
modificationsPhosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
Cellular localizationNucleus. Accumulates at sites of DNA damage.
- Information by UniProt
- DNA repair protein XRCC1 antibody
- RCC antibody
- X ray repair complementing defective repair in chinese hamster antibody
All lanes : Anti-XRCC1 antibody (ab235196) at 5 µg/ml
Lane 1 : U-2 OS whole cell lysate
Lane 2 : Molt4 whole cell lysate
Lane 3 : Human testis whole tissue lysate
Lane 4 : Mouse liver whole tissue lysate
Lysates/proteins at 35 µg per lane.
All lanes : Goat anti-Chicken IgG [H&L] at 1/40000 dilution
Predicted band size: 69 kDa
Exposure time: 20 seconds
100% methanol-fixed, 0.3% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling XRCC1 using ab235196 at 10 µg/ml, overnight at +4°C. Followed by a Donkey anti-Chicken IgG DyLight™ 488 conjugated preadsorbed secondary antibody at 5 µg/ml, for 1 hour at RT.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235196 has not yet been referenced specifically in any publications.