Anti-XRCC1 antibody (ab235196)
Key features and details
- Chicken polyclonal to XRCC1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgY
Overview
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Product name
Anti-XRCC1 antibody
See all XRCC1 primary antibodies -
Description
Chicken polyclonal to XRCC1 -
Host species
Chicken -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chinese hamster -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: U-2 OS and Molt4 whole cell lysate. ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 69 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 69 kDa. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. -
Sequence similarities
Contains 2 BRCT domains. -
Post-translational
modificationsPhosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
Sumoylated. -
Cellular localization
Nucleus. Accumulates at sites of DNA damage. - Information by UniProt
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Database links
- Entrez Gene: 7515 Human
- Entrez Gene: 22594 Mouse
- Entrez Gene: 84495 Rat
- Omim: 194360 Human
- SwissProt: P18887 Human
- SwissProt: Q60596 Mouse
- SwissProt: Q9ESZ0 Rat
- Unigene: 98493 Human
see all -
Alternative names
- DNA repair protein XRCC1 antibody
- RCC antibody
- X ray repair complementing defective repair in chinese hamster antibody
see all
Images
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All lanes : Anti-XRCC1 antibody (ab235196) at 5 µg/ml
Lane 1 : U-2 OS whole cell lysate
Lane 2 : Molt4 whole cell lysate
Lane 3 : Human testis whole tissue lysate
Lane 4 : Mouse liver whole tissue lysate
Lysates/proteins at 35 µg per lane.
Secondary
All lanes : Goat anti-Chicken IgG [H&L] at 1/40000 dilution
Predicted band size: 69 kDa
Exposure time: 20 seconds -
100% methanol-fixed, 0.3% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling XRCC1 using ab235196 at 10 µg/ml, overnight at +4°C. Followed by a Donkey anti-Chicken IgG DyLight™ 488 conjugated preadsorbed secondary antibody at 5 µg/ml, for 1 hour at RT.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab235196 has been referenced in 1 publication.
- Li H et al. PARP1 Inhibitor Combined With Oxaliplatin Efficiently Suppresses Oxaliplatin Resistance in Gastric Cancer-Derived Organoids via Homologous Recombination and the Base Excision Repair Pathway. Front Cell Dev Biol 9:719192 (2021). PubMed: 34497808