Anti-XRCC1 antibody (ab9147)
Key features and details
- Rabbit polyclonal to XRCC1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-XRCC1 antibody
See all XRCC1 primary antibodies -
Description
Rabbit polyclonal to XRCC1 -
Host species
Rabbit -
Specificity
This antibody recognises human XRCC1 (x-ray cross complementing factor -1), a base excision repair protein. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Horse, Guinea pig, Hamster, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide (Human) conjugated to KLH - which represented aportion within the last 100 amino acids of the human X-ray Repair Cross Complementing Protein 1 (GenBank PID 11425942).
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General notes
Recently XRCC-1 has been shown to be physically associated with other DNA repair enzymes, including PARP, DNA ligase III and DNA polymerase beta.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using thepeptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nmof 1.4 equals 1.0 mg of IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 70 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 70 kDa). |
Target
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Function
Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. -
Sequence similarities
Contains 2 BRCT domains. -
Post-translational
modificationsPhosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
Sumoylated. -
Cellular localization
Nucleus. Accumulates at sites of DNA damage. - Information by UniProt
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Database links
- Entrez Gene: 616905 Cow
- Entrez Gene: 476447 Dog
- Entrez Gene: 7515 Human
- Entrez Gene: 84495 Rat
- Omim: 194360 Human
- SwissProt: P18887 Human
- SwissProt: Q9ESZ0 Rat
- Unigene: 98493 Human
see all -
Alternative names
- DNA repair protein XRCC1 antibody
- RCC antibody
- X ray repair complementing defective repair in chinese hamster antibody
see all
Images
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All lanes : Anti-XRCC1 antibody (ab9147) at 1 µg/ml
Lane 1 : Whole cell extract (50mcg) from mock treated HeLa cells.
Lane 2 : Whole cell extract (50mcg) from siRNA treated HeLa cells.
Predicted band size: 70 kDa
Observed band size: 76,90 kDa why is the actual band size different from the predicted?
Detection: ECL.
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab9147 has been referenced in 15 publications.
- Ding X et al. The role of H3K9me2-regulated base excision repair genes in the repair of DNA damage induced by arsenic in HaCaT cells and the effects of Ginkgo biloba extract intervention. Environ Toxicol 36:850-860 (2021). PubMed: 33378118
- Flach KD et al. Endonuclease FEN1 Coregulates ERa Activity and Provides a Novel Drug Interface in Tamoxifen-Resistant Breast Cancer. Cancer Res 80:1914-1926 (2020). PubMed: 32193286
- Zhang Z et al. Triptolide interferes with XRCC1/PARP1-mediated DNA repair and confers sensitization of triple-negative breast cancer cells to cisplatin. Biomed Pharmacother 109:1541-1546 (2019). PubMed: 30551406
- Zhang LH et al. Up-regulated Wnt1-inducible signaling pathway protein 1 correlates with poor prognosis and drug resistance by reducing DNA repair in gastric cancer. World J Gastroenterol 25:5814-5825 (2019). PubMed: 31636474
- Sun C et al. Mobile phone signal exposure triggers a hormesis-like effect in Atm(+/+) and Atm(-/-) mouse embryonic fibroblasts. Sci Rep 6:37423 (2016). WB ; Mouse . PubMed: 27857169