Product nameAnti-XRCC1 antibody [EPR4389(2)]
See all XRCC1 primary antibodies
DescriptionRabbit monoclonal [EPR4389(2)] to XRCC1
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IHC-Frmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human XRCC1 aa 1-100 (N terminal). The exact sequence is proprietary.
- WB: HeLa, A375, Saos-2, PC-12 and NIH/3T3 cell lysates. Mouse brain and kidney tissue lysate IHC-P: Human testis tissue. ICC/IF: HeLa, PC-12 and NIH/3T3 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Purine and pyrimidine synthesis
Our Abpromise guarantee covers the use of ab134056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 69 kDa.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
FunctionCorrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
Sequence similaritiesContains 2 BRCT domains.
modificationsPhosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
Cellular localizationNucleus. Accumulates at sites of DNA damage.
- Information by UniProt
- DNA repair protein XRCC1 antibody
- RCC antibody
- X ray repair complementing defective repair in chinese hamster antibody
All lanes : Anti-XRCC1 antibody [EPR4389(2)] (ab134056) at 1/2000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse kidney lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 4 : NIH/3T3(Mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated(Pierce) at 1/1000 dilution
Predicted band size: 69 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Blocking/Diluting buffer: 5% NFDM /TBST
Lanes 1-2: 3 minutes
Lanes 3-4: 20 seconds
Immunocytochemistry/Immunofluorescence analysis of PC-12(Rat adrenal gland pheochromocytoma) labelling with ab134056 at a dilution of 1/50 dilution (4.66 µg/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) (1/1000 dilution (2 µg/mL)) was used as the secondary antibody. The cells were co-stained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Nuclei counterstained with DAPI (blue). Control: PBS instead of the primary antibody.
Immunocytochemistry/Immunofluorescence analysis of NIH/3T3(Mouse embryonic fibroblast) labelling with ab134056 at a dilution of 1/50 (4.66 µg/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 dilution (2 µg/mL)) was used as the secondary antibody. The cells were co-stained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Nuclei counterstained with DAPI (blue). Control: PBS instead of the primary antibody.
Immunohistochemistry (Frozen sections) analysis of mouse testis tissue labeling XRCC1 with ab134056 at 1/100. Tissue was fixed in paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. Heat mediated antigen retrieval was performed. Prior to blocking with BSA, sections were placed into a pH 6 solution of 0.002 M citric acid and 0.008 M sodium citrate and incubated in a decloaking chamber at 125°C for 30 seconds, then 90°C for 10 seconds. ab150062, an Alexa Fluor® 555 conjugated anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).
All lanes : Anti-XRCC1 antibody [EPR4389(2)] (ab134056) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A375 cell lysate
Lane 3 : Saos-2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 69 kDa
Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling XRCC1 with ab134056 at 1/250 dilution.
ab134056 staining XRCC1 in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.
Immunofluorescent analysis of HeLa cells labelling XRCC1 with ab134056 at 1/100 dilution.
This product has been referenced in:
- Niedenberger BA & Geyer CB Advanced immunostaining approaches to study early male germ cell development. Stem Cell Res 27:162-168 (2018). IHC-P ; Mouse . Read more (PubMed: 29475796) »
- Li M et al. APE1 deficiency promotes cellular senescence and premature aging features. Nucleic Acids Res 46:5664-5677 (2018). Read more (PubMed: 29750271) »