Overview

  • Product name

    Anti-XRCC1 antibody [EPR4389(2)]
    See all XRCC1 primary antibodies
  • Description

    Rabbit monoclonal [EPR4389(2)] to XRCC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IHC-Frmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human XRCC1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, A375, Saos-2, PC-12 and NIH/3T3 cell lysates. Mouse brain and kidney tissue lysate IHC-P: Human testis tissue. ICC/IF: HeLa, PC-12 and NIH/3T3 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 69 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
IHC-Fr 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
    • Sequence similarities

      Contains 2 BRCT domains.
    • Post-translational
      modifications

      Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
      Sumoylated.
    • Cellular localization

      Nucleus. Accumulates at sites of DNA damage.
    • Information by UniProt
    • Database links

    • Alternative names

      • DNA repair protein XRCC1 antibody
      • RCC antibody
      • X ray repair complementing defective repair in chinese hamster antibody
      • X ray Repair Complementing Defective Repair in Chinese Hamster Cells antibody
      • X ray repair complementing defective repair in chinese hamster cells 1 antibody
      • X ray repair cross complementing 1 antibody
      • X ray repair cross complementing protein 1 antibody
      • X ray repair, complementing defective, repair in Chinese hamster antibody
      • X-ray repair cross-complementing protein 1 antibody
      • XRCC 1 antibody
      • Xrcc1 antibody
      • XRCC1_HUMAN antibody
      see all

    Images

    • All lanes : Anti-XRCC1 antibody [EPR4389(2)] (ab134056) at 1/2000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Mouse kidney lysate
      Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
      Lane 4 : NIH/3T3(Mouse embryonic fibroblast) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated(Pierce) at 1/1000 dilution

      Predicted band size: 69 kDa
      Observed band size: 85 kDa
      why is the actual band size different from the predicted?



      Blocking/Diluting buffer: 5% NFDM /TBST

      Exposure time:
      Lanes 1-2: 3 minutes
      Lanes 3-4: 20 seconds

    •  Immunocytochemistry/Immunofluorescence analysis of PC-12(Rat adrenal gland pheochromocytoma) labelling with ab134056 at a dilution of 1/50 dilution (4.66 µg/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) (1/1000 dilution (2 µg/mL)) was used as the secondary antibody. The cells were co-stained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Nuclei counterstained with DAPI (blue). Control: PBS instead of the primary antibody.


       

    • Immunocytochemistry/Immunofluorescence analysis of NIH/3T3(Mouse embryonic fibroblast) labelling with ab134056 at a dilution of 1/50 (4.66 µg/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 dilution (2 µg/mL)) was used as the secondary antibody. The cells were co-stained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Nuclei counterstained with DAPI (blue). Control: PBS instead of the primary antibody.

    • Immunohistochemistry (Frozen sections) analysis of mouse testis tissue labeling XRCC1 with ab134056 at 1/100. Tissue was fixed in paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. Heat mediated antigen retrieval was performed. Prior to blocking with BSA, sections were placed into a pH 6 solution of 0.002 M citric acid and 0.008 M sodium citrate and incubated in a decloaking chamber at 125°C for 30 seconds, then 90°C for 10 seconds. ab150062, an Alexa Fluor® 555 conjugated anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).

      See Abreview

    • All lanes : Anti-XRCC1 antibody [EPR4389(2)] (ab134056) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A375 cell lysate
      Lane 3 : Saos-2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 69 kDa

    • Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling XRCC1 with ab134056 at 1/250 dilution.
    • ab134056 staining XRCC1 in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.

      See Abreview

    • Immunofluorescent analysis of HeLa cells labelling XRCC1 with ab134056 at 1/100 dilution.

    References

    This product has been referenced in:

    • Niedenberger BA & Geyer CB Advanced immunostaining approaches to study early male germ cell development. Stem Cell Res 27:162-168 (2018). IHC-P ; Mouse . Read more (PubMed: 29475796) »
    • Li M  et al. APE1 deficiency promotes cellular senescence and premature aging features. Nucleic Acids Res 46:5664-5677 (2018). Read more (PubMed: 29750271) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Testis, adult)
    Permeabilization
    Yes - 0.1% Triton X-100 in PBS
    Specification
    Testis, adult
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Paraformaldehyde

    Mr. Bryan Niedenberger

    Verified customer

    Submitted Sep 15 2016

    Application
    Western blot
    Sample
    Zebrafish Cell lysate - whole cell (Whole embryo - 24hpf)
    Gel Running Conditions
    Non-reduced Denaturing (4-20%)
    Loading amount
    50 µg
    Specification
    Whole embryo - 24hpf
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

    Mr. Stephen Moore

    Verified customer

    Submitted Dec 05 2014

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Fixative
    Paraformaldehyde

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Jan 23 2014

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