Overview

  • Product name

    Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free
    See all XRCC1 primary antibodies
  • Description

    Rabbit monoclonal [EPR4389(2)] to XRCC1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IHC-Frmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human XRCC1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, A375, Saos-2, PC-12 and NIH/3T3 cell lysates. Mouse brain and kidney tissue lysate IHC-P: Human testis tissue. ICC/IF: HeLa, PC-12 and NIH/3T3 cells.
  • General notes

    Ab226150 is the carrier-free version of ab134056. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab226150 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab226150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 69 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
    • Sequence similarities

      Contains 2 BRCT domains.
    • Post-translational
      modifications

      Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
      Sumoylated.
    • Cellular localization

      Nucleus. Accumulates at sites of DNA damage.
    • Information by UniProt
    • Database links

    • Alternative names

      • DNA repair protein XRCC1 antibody
      • RCC antibody
      • X ray repair complementing defective repair in chinese hamster antibody
      • X ray Repair Complementing Defective Repair in Chinese Hamster Cells antibody
      • X ray repair complementing defective repair in chinese hamster cells 1 antibody
      • X ray repair cross complementing 1 antibody
      • X ray repair cross complementing protein 1 antibody
      • X ray repair, complementing defective, repair in Chinese hamster antibody
      • X-ray repair cross-complementing protein 1 antibody
      • XRCC 1 antibody
      • Xrcc1 antibody
      • XRCC1_HUMAN antibody
      see all

    References

    ab226150 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab226150.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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